Objective To explore antioxidative effects of quercetin on damage induced by H_2O_2 in NIH-3T3 cells.Methods The NIH-3T3 cells in logarithmic growth phase incubated in DMEM were divided into 4 groups as follows:(1)Q pre-protection group(Q_1);prior to incubation with 0.5mmol/LH_2O_2 for 30 min,the NIH-3T3 cells were incubated with 50μmol/L quercetin for 24h;(2)Q post-protection group(Q_2):after incubation with 0.5mmol/L H_2O_2 for 30 min,the NIH-3T3 cells were incubated with 50umol/L quercetin for 24h;(3)H_2O_2 damage group(H_2):the NIH-3T3 cells were incubated with 0.5mmol/L H_2O_2 for 30 rain followed by subsequent incubation with 10% FBS for 24h;(4)The control group(C):the NIH-3T3 cells were incubated with DMEM medium supplemented with 10% FBS.The apoptosis was detected by DNA ladder and TUNEL.The expressions of Bcl-2,Bax,Caspase-3,HSP-70 and NF-kB were demonstrated with immunocytochemistry and Western blotting methods.The NIH-3T3 cell survival rate was detected by MTT assay.Results The apoptosis in the Q_1 group was decreased than that in the Q_2 and H_2 groups.The Q_1 group compared with Q_2 and H_2 groups,the expressions of Bcl-2 was increased;Bax,Caspase-3,HSP-70 and NF-kB were attenuated;and the NIH-3T3 cell survival rate was promoted.Conclusion The antioxidative effect of quercetin on damage in the NIH-3T3 cells may act through down-regulated expressions of NF-kB and up-regulated expression of HSP-70.

应用超短波胸部对置法作用于脑血管病患者胸腺部位,通过测定治疗前后外周血肿瘤坏死因子α(tumor necrosisfactroralpha,TNF-α)含量,观察超短波治疗对脑血管病患者体内TNF-α含量的影响.

超短波胸部对置作用于脑血管病患者胸腺部位.测定治疗前、后肘静脉血清中可溶性白细胞介素2受体(SIL-2R)含量的变化,观察超短波对脑血管病患者免疫功能的影响,为该类患者探讨一种有益的治疗.