目的 研究灵芝乙醇提取物是否通过脾酪氨酸激酶(Syk)影响胰腺癌细胞SW1990的增殖、迁移和侵袭.方法 胰腺癌细胞SW1990给予25、50、100μg/ml剂量的灵芝乙醇提取物,采用噻唑蓝(MTT)比色法检测细胞存活,平板克隆测定细胞克隆形成,Transwell小室法评估细胞迁移和侵袭,免疫印迹实验(western blot)分析细胞核相关抗原Ki67(Ki67)、上皮型钙黏附蛋白(E-cadherin)、神经型钙黏附蛋白(N-cadherin)及Syk表达.在SW1990细胞中转染pcDNA3.1-Syk,或转染pcDNA3.1-Syk并给予100μg/ml浓度的灵芝乙醇提取物,利用上述方法考察细胞的增殖、迁移和侵袭变化.结果 低、中、高剂量的灵芝乙醇提取物明显降低细胞存活率、克隆形成数、迁移细胞数、侵袭细胞数、Ki67、N-cadherin蛋白表达水平,并显著提高E-cadherin、Syk蛋白表达水平,均呈剂量依赖性(P<0.05).Syk过表达明显减少细胞的细胞存活率、克隆形成率、迁移细胞数、侵袭细胞数、Ki67、N-cadherin蛋白表达水平,显著增加E-cadherin蛋白表达水平(P<0.05).Syk过表达后,灵芝乙醇提取物对SW1990细胞存活、克隆形成、迁移、侵袭、Ki67、N-cadherin蛋白表达的抑制作用和其对细胞E-cadherin蛋白表达的促进作用被增强.结论 灵芝乙醇提取物通过上调Syk,抑制胰腺癌细胞SW1990的增殖、迁移和侵袭能力.

目的:探讨对急性化脓性胆囊炎患者的手术治疗中超声引导下经皮经肝胆囊穿刺引流与开腹胆囊切除术治疗的临床效果.方法:随机选取我院2017年5月至2019年12月内收治的80例急性化脓性胆囊炎患者,所有患者以治疗手术差异分为参照组(50例,腹腔镜胆囊切除术)和研究组(30例,超声引导下经皮经肝胆囊穿刺引流),对比分析两组患者的手术相关指标(术中出血量、手术时长、住院时长、切口长度)和术后并发症(创口感染、腹腔感染、腹腔出血、胆汁渗漏)的发生几率.结果:研究结果显示,研究组患者手术相关指标明显优于参照组,且研究组患者的术后并发症总发生率为6％(3/30)明显低于参照组20％(10/50),所有对比均差异明显(P<0.05).结论:在对炎症较重的急性化脓性胆囊炎高龄多合并症的患者临床治疗时采用超声引导下经皮经肝胆囊穿刺引流,相比腹腔镜胆囊切除术更加安全有效,临床应用价值更高.

Objective To discuss effect of IL-10, TGFβ1 genetically modified DC on mice liver transplantation immunologic tolerance. Methods IL-10, TGFβ1 genetically modified imDC was infused into mice bodies before transplantation. Liver function, IL-12 level and rejection reaction were observed. Results Serum IL-10 levels of combined transfection group at 3 d and 7 d after transplantation were lowest, and apparently lower than mDC group and control group(P<0.01). Portal area cell apoptosis of combined transfection group was most remarkable at 3 d, 7 d and 10 d after transplantation, and next were IL-10 transfection group and TGFβ1 transfection group. They were all more remarkable than control group, mDC group and imDC group(P<0.01). Combined transfection group acute rejection level was lowest, and next to it were TGFβ1 transfection group and IL-10 transfection group. mDC group and control group acute rejection levels were most. There were significant difference compared in groups(P<0.01). After transplantation, mDC group and control group liver functions were worst, and that of combined transfection group was best. Conclusion IL-10, TGFβ1 genetically modified DC can induce mice liver transplantation immunologic tolerance.