用已经制备的纯化的MCM5蛋白免疫新西兰大白兔,获得抗MCM5蛋白的多克隆抗体.重组蛋白MCM5既具有免疫原性又具有免疫反应性.

AIM:To study the structural property of the bioactive component from the fermentation broth of Streptomyces 139 which can inhibit IL-1R.METHODS:Polysaccharide 139A is extracted and purified by means of macroreticular resin Diaion HP-20, cation exchange and DEAE Sephadex A-25 chromatography. The sugar compositions were analyzed with GC, the linkage positions of 139A were determined by methylation and GC-MS.RESULTS:The bioactive component which can inhibit IL-1R is a kind of polysaccharides.CONCLUSION:The components of 139A repeat units are conjectured.