目的 探讨干燥综合征(SS)患者抗SSA抗体、抗SSB抗体及IgG与唇腺活检病理(LSGB)的关系.方法 选取85例疑似SS患者作为研究对象,取唇腺组织行病理检查,根据LSGB结果分为阳性组(LSGB≥3级,n=53)和阴性组(LSGB<3级,n=32),比较两组患者唇腺组织学变化、血清抗SSA抗体、抗SSB抗体及IgG表达;采用Logistic回归模型分析LSGB与抗SSA抗体、抗SSB抗体、IgG的关系;LSGB阳性组患者根据唇腺病理损伤程度分为轻度组(n=23)和重度组(n=30),比较两组患者抗SSA抗体、抗SSB抗体及IgG水平,采用Spearman法分析抗SSA抗体、抗SSB抗体、IgG与唇腺损伤程度的相关性.结果 85例疑似SS患者LSGB阳性53例,且轻度共23例(43.40％)、重度共30例(56.60％);所有疑似SS患者抗SSA抗体、抗SSB抗体、IgG比较,阳性组>阴性组,差异有统计学意义(P<0.05);二元Logistic回归分析显示,抗SSA抗体、抗SSB抗体及IgG升高会对唇腺病理损伤产生影响,差异有统计学意义(P<0.05);轻、重度组SS患者抗SSA抗体、抗SSB抗体及IgGg/L比较,轻度组

Objective To investigate the role of neuroglobin (NGB) and vascular endothelial growth factor (VEGF) regulated by hypoxia-inducible factor-1 (HIF-1) in promoting angiogenesis after cerebral contusion and laceration.Methods Proteomic technology was used to compare differentially expressed proteins in the frontal lobe between patients with cerebral contusion and laceration and those with non-traumatic brain injury,and bioinformatics technology was used for the analysis and identification of differentially expressed proteins.Immunohistochemistry and ELISA were used to measure the expression of HIF-1,NGB,and VEGF in cerebral tissue and serum.Results The 2-dimensional gel electrophoresis profile was established for brain tissues from patients with cerebral contu sion and laceration or non-traumatic brain injury,and 7 differentially expressed proteins were identified,including HIF-1,NGB,and VEGF.Immunohistochemistry showed that the patients with cerebral contusion and laceration had higher expression of HIF-1,NGB,and VEGF in brain tissue than those with non-traumatic brain injury.The results of ELISA showed that the patients with cerebral contu sion and laceration had higher expression of HIF-1,NGB,and VEGF in serum than those with non-traumatic brain injury.Conclusions HIF-1 regulates the release of two target genes NGB and VEGF after cerebral contusion and laceration and thus plays an important role in cerebral angiogenesis.Changes in the serum levels of HIF-1,NGB,and VEGF can be used as a reference for clinical evaluation and monitoring of the severity of hypoxic-ischemic encephalopathy.They also have important significance in the diagnosis,treatment,and outcome of cerebral contusion and laceration and await in-depth research.