AIM:To observe whether Salvianolate Injection has a therapeutic effect on colitis and to discuss the possible mechanisms involved.METHODS:50 Balb/c mice were randomly divided into a control group,a model group,and low-,medium-,and high-dose interventional groups.Colitis was induced by oral administration of 5％ DSS for 7 d.Indices were determined 14 d after colitis induction.RESULTS:Treatment with Salvianolate Injection could reduce colonic inflammation induced with DSS (P ＜ 0.01).Immunohistochemistry analysis showed that treatment with different doses of Salvianolate Injection significantly reduced CD40/CD40L protein expression compared to the model group (CD40:20.50 μm2 ±1.517 μm2,15.50 μm2 ± 4.680 μm2,13.33 μm2 ±0.816 μm2 vs 30.33 μm2 ± 4.227 μm2,all P ＜ 0.01;CD40L:23.17 μm2 ± 2.714 μm2,15.50 μr2 ± 2.739μm2,14.17 μm2 ± 4.262 μm2 vs 27.83 μm2 ± 3.710μm2,all P ＜ 0.01).RT-PCR analysis showed that treatment with different doses of Salvianolate Injection significantly reduced CD40/CD40L mRNA expression compared to the model group (CD40:1.20％ ± 0.30％,0.78％ ± 0.26％,0.41％ ±0.15％ vs 1.78％ ± 0.41％,all P ＜ 0.01; CD40L:2.59％± 1.68％,1.75％ ± 0.64％,1.11％ ± 0.53％ vs 3.54％ ±0.85％,all P ＜ 0.01).The concentration of sCD40L in medium was decreased in the medium-and high-dose interventional groups compared to the model group (2.12 ng/mL ± 0.18 ng/mL,1.96ng/mL ± 0.12 ng/mL vs 5.62 ng/mL ± 0.36 ng/mL,P ＜ 0.01).CONCLUSION:Salvianolate Injection can exert a therapeutic effect on colitis in mice possibly by reducing sCD40L and thus restraining the CD40/CD40L pathway.

Objective To understand the pre-operative nutritional situation in liver transplantation patients and explore its correlation with post-operative infections. Methods The medical histories, physical examinations, and laboratory test results of 81 patients who underwent liver transplantation were collected and analyzed and their nutrition statuses were assessed before operations. After the operations, there infection events were recorded and the correlation between pre-operative nutritional situation and post-operative infections were analyzed. Results Body mass index showed the malnutrition rate was 44.9% in these patients before operation, while the malnutrition rate was 69.1% by subjective global assessment (SGA), 80.2% by measuring the amount of serum albumin (ALB), and 96.1% by measuring the amount of pre-albumin. Single variable analysis showed malnutrition status (P0.01) and ascitic fluid status (P﹤0.001) were the independent risk factors of infections, while multiple variable analysis showed malnutrition grading by SGA (OR=10.057; 95% CI: 2.810-35.997) and malnutrition by measuring ALB (OR=0.324; 95%CI: 0.112-0.938) were correlated with post-operative infections. Conclusions Liver transplantation patients have severe malnutrition beforeoperation, which may influence post-operative infections·A comprehensive pre-operational nutritional assess-ment, especially SGA, is required·