目的 将通过已有的小鼠体外胚胎着床模型对annexin A2在胚胎着床中的功能进行相应的研究.方法 将孕第4天ICR小鼠囊胚与ICR小鼠的内膜组织进行体外共培养.比较子宫内膜经annexin A2抗体预处理以及未经处理之间,各组胚胎黏着率的差异,并在共培养后进行组织学研究.结果 小鼠子宫内膜经浓度为1.0μg/ml及0.1μg/ml的annexin A2抗体预处理后小鼠囊胚在其上的黏着率分别为45.6％及53.6％;而抗体浓度为1.0μg/ml的正常IgG对照及未经抗体处理的空白对照组的黏着率分别为55.4％及59.0％.经过1.0μg/ml的annexin A2抗体预处理组与两组对照组之间的黏着率比较,差异无统计学意义.免疫组化实验发现,经过annexin A2抗体预处理后,未见到annexin A2表达明显降低.结论 annexin A2在第4天的子宫内膜组织表达明显高于第1天的子宫内膜组织.运用抗体进行阻滞实验时并不能显著降低体外胚胎在子宫内膜组织的黏着率.

目的 运用生物素标记表面蛋白联合二维电泳技术发现小鼠子宫内膜腔上皮表面胚胎着床相关表面蛋白.方法 对孕第1天(不接受胚胎阶段)和第4天(接受胚胎阶段)小鼠宫腔注入生物素进行标记,将抽提纯化后的表面蛋白进行二维电泳比较,发现的差异表达点用质谱进行分析确定,并用免疫组化方式进行验证.结果 发现20个差异表达超过3倍的蛋白,其中接受胚胎阶段高表达蛋白13个,不接受胚胎阶段高表达蛋白7个.并用质谱发现确定了aminopeptidase N(APN)、annaxinA2和intergrin β1等蛋白.通过免疫组织化学方法验证了二维电泳的结果.结论 笔者通过生物素标记小鼠子宫内膜腔上皮表面,并联合二维电泳发现了一些在接受胚胎着床阶段和不接受胚胎阶段差异表达的表面蛋白以及一些和胚胎着床相关的重要蛋白.

Objective To confirm the three-dimentional in vitro embryo implantation model,the ICR mouse blastocyst was replaced by green fluorescent protien (GFP) mouse blastocyst in the study.Methods In vitro GFP and ICR embryo implantation was performed on ICR endometrium cultured.The attachment rates were compared.The GFP mouse blastocyst were checked by fluorescent microscopy,the histological study then followed.Results There was no significant different attachment rate between the GFP mouse blastocyst group (46.6％) and the ICR mouse blastocyst group (54.8％).The GFP mouse blastocyst can be seen with fluorescent microscopy after 28 and 40 hours' co-culture.The GFP mouse blastocyst can attach to ICR mouse endometrium.Conclusion The embryo from different mouse strain can attach to the mouse endometrium in the in vitro embryo implantation model.There may be information and molecular cross-talk between the GFP mouse embryoes and ICR mouse endometrium in this study.