目的 研究右美托咪定复合七氟醚行麻醉维持应用于儿童腺样体肥大切除手术的效果.方法 选取2018年6月～2020年5月收治的腺样体肥大患儿98例,均行腺样体切除术,其中49例采用七氟醚麻醉维持为对照组,49例采用右美托咪定复合七氟醚麻醉维持为复合组.比较两组麻醉前(T0)、气管插管时(T1)、术后即刻(T2)血流动力学指标[平均动脉压(MAP)、心率(HR)]、术后6 h躁动率、疼痛程度[面部表情量表法(FPS评分)]及补救镇痛药舒芬太尼用量.结果 T1、T2时复合组MAP、HR小于对照组(P<0.05);复合组躁动率6.12％小于对照组20.41％(P<0.05);复合组FPS评分低于对照组(P<0.05);复合组补救镇痛药舒芬太尼用量小于对照组(P<0.05).结论 右美托咪定复合七氟醚行麻醉维持应用于儿童腺样体肥大切除手术能维持术中血流动力学稳定,减少术后躁动情况发生,减轻疼痛程度,降低补救镇痛药舒芬太尼用量.
目的 探讨在基因水平上地佐辛对瑞芬太尼诱发痛觉过敏的大鼠脊髓Gs蛋白的影响.方法 成年雄性SD大鼠,体重200～250 g,12～14周龄,32只,随机分成4组8例,空白对照组(C组)、手术组(S组)、瑞芬太尼组(R组)、地佐辛组(D组);C组大鼠尾静脉持续泵注盐酸瑞芬太尼同体积的静脉用0.9％Nacl,持续60 min;S组大鼠麻醉后制备切口痛模型,同时尾静脉泵注生理盐水60 min;R组大鼠麻醉后,静脉注射地佐辛同体积生理盐水0.1 ml,随后制备切口痛模型,同时泵注瑞芬太尼1.3 μg/(kg·min)持续60 min;D组大鼠麻醉后静脉注射地佐辛1 mg/kg,随后制备切口痛模型后,同时持续静脉泵注瑞芬太尼1.3μg/(kg·min),持续60 min.检测4组大鼠给药前及给药后2h的热痛刺激潜伏期(PWTL)及大鼠腰膨大处脊髓中Gs蛋白mRNA表达水平.结果 (1)行为学结果:给药前各组大鼠PWTL无差异(P＞0.05).给药后,S组和R组PWTL短于C组(P＜0.05),D组PWTL与C组无差异(P ＞ 0.05);与S组相比,R组PWTL缩短,D组PWTL延长(P＜0.05);与R组相比,D组PWTL延长.(2)Gs蛋白mRNA表达结果:与S组相比,R组Gs mRNA表达上调(P＜0.05);与D组相比,R组Gs mRNA水平升高(P＜0.05).结论 地佐辛可抑制瑞芬太尼诱发大鼠痛觉过敏,同时脊髓Gs蛋白表达降低.
Objective To study the levels of S100βprotein in serum after ischemia-reperfusion injury in rats, and to evaluate the significance on early diagnosis of brain injury. Methods Sixteen male Sprague-Dawley rats weighing 200-250 g were randomly divided into 2 groups(n=8 each):sham operating group(S) and ischemia-reperfusion group(I/R);the model of cerebral ischemia-reperfusion injury in rats was induced by occlusion of bilateral common carotid arteries and basilar artery for 10 min and then reopening them for 6 h. When the experiment was completed , the vein blood samples were taken from internal jugular vein of rats, serum S100β protein levels were analyzed with enzyme linked immuno-sorbent assay (ELISA). Then animals were decapitated and brain water content was determined by dry-wet weight method on the left. Right brain was removed for ultrastrucrure examination. Results Compared S group, I/R group serum S100 βprotein level and brain water content were increased (P<0.05). I/R group injury degree of the cortex neurons in rats was serious. Conclusion S100βproteins could be used to evaluate brain injury after I/R injury.
