目的 了解湖州地区食源性腹泻病原谱的分布特征,可疑中毒食物范围和感染的季节分布为临床诊断和制定防控措施提供依据.方法 通过对湖州地区2016年食源性腹泻患者的粪便进行细菌病原学检测,并对检测结果进行分析.结果 510例食源性腹泻患者的粪便共分离得到病原菌110株,检出率21.57％,检出的病原菌包括致泻性大肠杆菌60株11.76％(60/510)、副溶血性弧菌41株8.04％(41/510)、沙门菌5株0.78％(5/510)、气单胞菌3株0.59％(3/510)、其它肠道病原菌2株0.40％(2/510),志贺氏菌未检出.510例食源性腹泻患者中主要可疑食物为肉类与肉制品24.2％,果蔬类占18.1％,混合多种食品占17.1％,水产品类占13.9％.结论 湖州地区食源性腹泻感染高发期为8月份,病原菌前三位是致泻性大肠杆菌,副溶血性弧菌,沙门菌,同时肉类和肉制品、果蔬类、混合多种食品和水产品是可能的污染食品.
目的 分析食源性腹泻患者副溶血弧菌(VP)季节分布及感染患者年龄特征、毒力基因、血清学分型.方法 对2014年1月至2016年12月急性腹泻患者粪便分离的125株副溶血弧菌进行常规培养、血清分型及PCR毒力基因检测.结果 副溶血弧菌感染发生有明显的季节性,夏秋季高发,尤以6～10月份为高峰期.感染患者中21～30岁占37.6％,31～40岁占24.8％.分离得到17个血清型,其中O3:K6占59.2％.经单重PCR进行毒力基因检测显示:tdh+/trh-分离株所占比例为88.0％,tdh-/trh-分离株为6.40％,检测到2株携带(tdh+/trh+)基因.结论 湖州地区副溶血性弧菌感染发生呈明显的季节分布特征,且感染的患者以青壮年居多,优势血清型为O3:K6;多数菌株携带tdh+/trh-毒力基因.
OBJECTIVE To investigate if baicalin was able to cure antibiotic-resistant plasmid in Acinetobacter baumanii,and affect minimum inhibitory concentrations(MIC) of antibiotics to bacteria.METHODS The growth curve of A.baumanii treated with baicalin was detected.16 strains of A.baumanii,gentamicin(GEN) and ciprofloxacin(CIP) resistance,were treated with baicalin for 20 hours.Before and after baicalin treatment,plasmids of A.baumanii were determined with alkaline lysis method,and MICs of GEN and CIP were measured by agar dilution method.RESULTS Baicalin inhibited the growth of A.baumanii.The plasmid curing rate was 37.5%(6/16) in 16 strains of A.baumanii with plasmids,after the treatment with baicalin(1 mg.mL 1).In 6 strains of A.baumanii with plasmid cured by baicalin,for medium or low level of GEN-resistance strains,MICs of GEN all decreased to sensitive level;for low level of CIP-resistance strains,MICs of CIP all dropped to sensitive level,and for some medium-level of CIP-resistance strains,MICs of CIP also dropped,but still above the resistance level.CONCLUSION Baicalin can eliminate plasmids of A.baumanii with low level of antibiotic-resistance,and make these strains recover the susceptibility to GEN and CIP,which shows an adjuvant treatment method for antibiotic-resistant A.baumanii infections.
