目的 探究G蛋白偶联受体35(GPR35)在前列腺癌患者血清中的水平变化,并评估其临床诊断效能.方法 以行前列腺穿刺活检术的59例患者为研究对象,依据病理诊断分为前列腺癌组(34例)和前列腺良性疾病组(25例),以同期体检健康者34例作为对照组.收集各组血清标本,采用酶联免疫吸附试验检测GPR35水平,化学发光法检测前列腺特异性抗原(PSA)、游离前列腺特异性抗原(fPSA)水平;比较GPR35在各组血清中的水平变化,进而分析血清GPR35水平与前列腺癌患者肿瘤最大径、淋巴结转移、膀胱精囊腺转移、神经侵犯、TNM分期和Gleason评分等的相关性;采用受试者工作特征(ROC)曲线分析评估GPR35单独及联合PSA和fPSA检测诊断前列腺癌的效能.结果 前列腺癌组血清GPR35水平[1.01(0.72,1.28)ng/mL]显著高于前列腺良性疾病组[0.61(0.36,0.82)ng/mL]和对照组[0.57(0.35,0.94)ng/mL](P<0.05).不同淋巴结转移、精囊腺膀胱转移和神经侵犯情况的前列腺癌患者血清GPR35水平比较差异有统计学意义(P<0.05).血清GPR35单独检测诊断前列腺癌的曲线下面积(AUC)为0.780,灵敏度为88.24％,特异度为57.63％.GPR35、PSA、fPSA三者联合检测诊断前列腺癌的AUC为0.927,灵敏度为98.31％,特异度为73.53％.结论 GPR35对前列腺癌临床诊断具有一定价值,GPR35、PSA、fPSA联合检测可提高其临床诊断效能,GPR35有可能是新型的前列腺癌诊断生物标志物.

Objective To study tumor vessel appearance and dynamic mechanism of hepatic carcinoma with color Doppler imaging(CDI).Methods 120 masses of hepatic carcinoma were examined with Doppler ultrasonography.The grading mode and the abnomal appearance of the tumor vessel were observed with CDFI and CDE.The results were compared with that of DSA.The Doppler parameters including velocity of peak systole(PS) and resistent index(RI) of them were studied.Results Of the 120 masses,The most abnomal appearance and grading mode of the tumor vessel were detected with CDFI and CDE.The more blood supple in the tumor,the higher was the PS.The PS of the twists and stenosis type was higher than that of the punctiform or short streak type.The group of vascular pool have lowest PS.High RI was detected in all groups in which the vascular pool has the highest RI(0.83).Among the different grading mode vessel,the PS of AVF was highest,then was the branch and the insert type,and lastly was the net type(P<0.05).The RI of AVF was the lowest(0.51).Conclusion Color Doppler ultrasonography can clearly detect the abnomal appearance and grading mode of the tumor vessel,which diversify the parameter of the blood vessel dynamic mechanism.

子宫内膜癌是女性生殖器官的恶性肿瘤之一,多发于绝经后出血妇女.近年来由于雌激素替代疗法的应用增多,该病发病率有上升趋势.现对我院2001年3月～2003年8月经阴道彩色多普勒超声(以下简称阴彩超)检查并经手术病理证实的36例子宫内膜癌作总结性分析,并与经腹部超声扫查比较,以探讨经阴道及经腹部彩色多普勒超声检查相结合对子宫内膜癌的诊断价值.