Objective To observe the effect of Weici combined with electric acupuncture on E 2 /P,prolactin(PRL), testosterone(T),ER expression and mammary gland tissue morphous in rats with mammary gland hyperplasia in order to explore its action mechanism .Methods The 40 Wistar rats were randomly divided into 4 groups:normal control group ,model group,acupuncture group and tamoxifen ( TAM) group.The animal models with breast hyperplasia were established by intramuscular injection with diethylstilbestrol +progesteroner .The rats in acupuncture group were fixed and acupunctured (Weici) in the second pair of breasts,and connected with the electric acupuncture instrument for 30 min,moreover, Danzhong acupuncture point was acupunctured for 30min;the rats in TAM group were given tamoxifen 1.8mg/kg by gavage,once a day for 30 days,after last treatment,the serum levels of E2,P,PRL,T were detected,and the ratio of E2/P was calculated,the second pair of breasts were taken to be HE stained , then the changes of breast tissue morphous were observed under light microscope,and the expression levels of ER in breast tissue were detected by immunohistochemisty .Results After treatment, the ratio of E2/P in acupuncture group and TAM group was significantly lower than that in model group ( P <0.05 );the serum levels of PRL in acupuncture group and TAM group were obviously lower than those in model group ( P <0.05), moreover,there were significant differences between acupuncture group and TAM group ( P <0.05).The serum levels of T in acupuncture group and TAM group were significantly lower than those in model group ( P <0.05).The pathological changes of breast tissue in acupuncture group were similar to those in TAM group , however , which were obviously improved , as compared with those in model group .The expression levels of ER of breast tissue in TAM group were significantly decreased , furthermore,the AOD value was obviously lower than that of normal control group ( P <0.05).Conclusion Weici combined with electric acupuncture can decrease nipple height and nipple diameter , improve disorder state of E2/P, PRL, T and pathological morphous of breast tissue ,and reduce ER expression levels in breast tissue of rats with breast hyperplasia .