目的 探讨大黄素(emodin,DHS)对去卵巢大鼠骨量流失的影响,并探索可能的机制.方法 通过双侧去卵巢建立骨质疏松大鼠模型;随后随机分为假手术组(Sham)、去卵巢组(OVX)以及大黄素组(DHS),每组10只;其中DHS组大鼠接受大黄素[90 mg/(kg·d)]治疗12周;待治疗结束后使用Micro-CT、HE染色切片、骨代谢指标、以及蛋白质印迹观察治疗效果以及可能的机制.结果 治疗12周后,与OVX组相比,Micro-CT和HE染色切片结果显示DHS组的大鼠骨小梁数量和骨密度得到明显改善.DHS组大鼠BMD、TV/BV、Tb.N、Tb.Th和Tb.Sp较OVX组明显改善(P<0.05).与OVX组相比,DHS组的BALP水平明显升高(P<0.05);而TRACP-5b和β-CTX水平显著降低(P<0.05).和OVX组比较,DHS组OPG表达水平上调(P<0.05),而RANKL和β2AR表达水平下调(P<0.05).结论 大黄素可以通过降低 β2AR表达和激活OPG/RANKL信号通路介导对去卵巢大鼠骨量流失的保护作用.
Objective To investigate the effect of polypoid lesions of gallbladder (PLG) and related risk factors of the disease. Methods The clinical data of five hundred and ten patients were retrospectively analyzed, who were diagnosed with PLG by ultrasound in the Affiliated Hospital of Southwest Medical University from March 2010 to January 2016 and followed up in the Department of Hepatobiliary Surgery. Results Among the 510 patients with PLG, 293 (57.5%) had single polyp, and the follow-up time was 15 (2-58) months. The diameter of 300 (58.8%) polyps increased by 2 (1-10) mm, with an average growth rate of 0.4 mm/year. There was no change in diameter in 196 cases (38.4%), decreased in eight cases and disappeared in six cases. Univariate analysis and multivariate Logistic regression analysis showed that alcohol consumption and poor sleep quality were risk factors for PLG diameter increasing, and the difference was statistically significant (P<0.01). The drinkers were 2.615 times more likely to have an increased PLG diameter than that of non-drinkers. The risk of increasing polyps of poor sleeping subjects was 2.282 times higher than that of subjects with normal sleep. Conclusions The growth rate of PLG patients in Luzhou region is slow. Drinking alcohol and poor sleeping quality may be risk factors for gallbladder polyp growth. Key words: Gallbladder diseases; Polyps; Risk factors; Natural history
目的 探讨脐动脉、大脑中动脉血流联合胎心监护对预测重度子痫前期患者围生儿结局的价值.方法 选取2017年1月-2018年1月该院收治100例住院分娩的单胎重度子痫前期患者及其分娩的新生儿为观察组,同期选取100名孕龄相近未合并重度子痫前期等其他并发症的正常孕妇及其分娩的新生儿作为对照组.对所有患者行产科B超检查脐动脉血流、大脑中动脉血流S/D值及胎心监测,记录并比较围生结局,分析脐血流、大脑中动脉血流S/D值及胎心监测对预测重度子痫前期围生儿结局的价值.结果 观察组患者胎心监护及脐动脉、大脑中动脉血流S/D值异常率均高于对照组(P＜0.05);观察组早产、胎儿窘迫、新生儿窒息、围生儿死亡、低体质量儿及产后出血发生率均高于对照组(P＜0.05);观察组围生儿预后不良36例,产前予以胎心监护、脐动脉及胎儿大脑中动脉血流S/D值监测,其中胎心监测异常26例,有围生儿预后不良20例;胎心监测正常74例,有围生儿预后不良16例;脐动脉血流S/D值异常31例,有围生儿预后不良23例;脐动脉血流S/D值正常69例,有围生儿预后不良13例;大脑中动脉血流S/D值异常40例,有围生儿预后不良25例,大脑中动脉血流S/D值正常60例,有围生儿不良预后11例;胎心监测联合脐动脉、大脑中动脉血流S/D值,三者任一异常有44例,其中有围生儿预后不良32例,三者均正常的有56例,有围生儿预后不良仅4例.结论 脐动脉、大脑中动脉S/D值及胎心监护对重度子痫前期围生儿结局有一定预测作用,但单独指标有一定假阳性率,三者联合监测,灵敏度、准确度均提高,假阳性率远远低于单独监测,对于重度子痫前期患者围生儿预后有更好的预测作用.
