Objective:To explore the effects of various glycotoxicity pathway inhibitors on cellular mitochondria, and provide better therapeutic strategy for the effective treatment of diabetic vascular complications.Methods:In this study, we inhibited polyol, hexosamine, AGE and PKC pathway with epalrestat, azaserine, aminoguanidine, sotrastaurin, respectively in human umbilical vein endothelial cell (HUVEC) before and after high glucose treatment. The mitochondria ROS was determined by flow cytometry and mitochondrial division was observed by confocal laser microscopy.Results:The mitochondrial ROS and fragmentation of HUVEC were significantly increased by high glucose stimulation. After high glucose incubation, the mitochondrial ROS level and fragmentation did not decrease significantly. However, pre-addition of glucose-toxic pathway inhibitors before hyperglucose stimulation can effectively reduce mitochondrial ROS levels and mitochondrial division in HUVEC.Conclusions:Inhibition of the glucotoxic pathway after high glucose stimulation cannot effectively alleviate mitochondrial damage. When the glycotoxic pathway is inhibited in advance, mitochondrial damage can be effectively alleviated. It is expected to provide better therapeutic strategies for the effective treatment of diabetic vascular complications by clarifying the effects of various glucotoxic pathway inhibitors on cellular mitochondria.

目的:探讨医用三氧治疗下肢动脉硬化闭塞症(ASO)的机理和临床疗效.方法 :将61例下肢动脉硬化闭塞症患者(ASO)随机分为治疗组31例行自体三氧血回输治疗,对照组30例行常规"前列地尔注射液"治疗.治疗前后分别进行血液流变学指标的检测,比较两组的临床疗效及治疗前后血液流变学的变化.结果 :治疗组总有效率为96.7％,疗效优于对照组的87.9％(P<0.05);治疗组患者临床症状和体征及血液流变学有明显的改善,两组比较均有显著性差异(P<0.05).结论 :自体三氧血回输治疗下肢动脉硬化闭塞症疗效显著,有改善下肢血液循环的功能.