目的 分析高频超声评估针灸电针治疗产后女性腹直肌分离(diastasis recti abdominis,DRA)的效果.方法 选取2020年1月-2020年9月产后42d在本院门诊复查的DRA产后女性178例,按照治疗方法的不同分为对照组(未采取任何治疗措施)55例、观察A组(给予常规治疗,如仿生物电刺激治疗)61例、观察B组(在常规治疗的基础上给予针灸电针治疗)62例.采用高频超声测量患者的腹直肌间距(IRD),比较三组患者治疗前后的IRD、腹围恢复情况,以及治愈率.结果 重复测量显示,IRD以时点、组间、时点和组间交互作用比较,差异均有统计学意义(P＜0.05);事后LSD-t显示,三组IRD比较:治疗3个疗程＜治疗2个疗程＜治疗1个疗程＜对照组,且治疗1个疗程、2个疗程、3个疗程观察B组＜观察A组＜对照组,差异均有统计学意义(P＜0.05).治疗后三组脐上围、脐围、脐下围比较:观察B组＜观察A组＜对照组,差异均有统计学意义(P＜0.05).三组治愈率比较:观察B组(59.68%)＞观察A组(31.15%)＞对照组(9.09%),差异均有统计学意义(P＜0.05).结论 在常规治疗的基础上给予针灸电针治疗产后DRA产后女性,可有效提高腹围恢复情况,提高治愈率;高频超声能够评估产后腹直肌的分离情况,可评估为针灸电针治疗疗效,为针灸治疗提供参考.

目的 观察超声微泡造影剂携带pcDNA-STC1转染人宫颈癌细胞HeLa后增殖、转移、侵袭,血管生成以及PI3K/AKT信号通路变化.方法 利用GEPIA数据库分析宫颈癌组织STC1的表达水平、患者的预后生存期.选择2019年1-10月在我院诊治的宫颈癌患者80例作为研究对象,收集癌组织和癌旁组织,qRT-PCR检测STC1 mRNA的表达,并分析STC1 mRNA表达与患者临床病理因素的关系;HeLa细胞分组:空白对照组:HeLa细胞正常培养,不做任何处理.阴性对照组(HeLa-pcDNA-NC):5％微泡浓度+2μg空载质粒+无血清DMEM培养基.实验组(HeLa-pcDNA-STC1):5％微泡浓度+2μg pcDNA-STC1质粒+无血清DMEM培养基.MTT法及癌细胞单克隆形成数目检测细胞增殖能力;划痕实验检测细胞迁移能力;Transwell小室实验检测细胞侵袭能力;Matrigel体外成管实验检测细胞的血管生成能力;Western blot法检测p-PI3K、p-AKT、基质金属蛋白酶-2(MMP-2)/基质金属蛋白酶-9(MMP-9)、血管内皮生长因子(VEGF)、血管生成素2(Ang2)的表达.结果 GEPIA数据库显示宫颈癌组织STC1的表达水平低于正常组织,且预后生存期与STC1的表达水平呈负相关;与癌旁正常宫颈组织相比,宫颈癌组织和STC1 mRNA下调,且miR-574-5p水平与STC1水平呈负相关,与肿瘤分化程度、FIGO分期、淋巴结转移有明显的相关性,与空白对照组和阴性对照组相比,实验组48 h、72 h、96 h OD值降低,细胞克隆数极少,迁移率降低,细胞侵袭数减少,血管生成能力下降,MMP-2、MMP-9、VEGF、Ang2、p-PI3K、p-AKT蛋白表达下调(P<0.05).结论 超声微泡造影剂携带pcDNA-STC1转染能够明显抑制人宫颈癌细胞HeLa增殖、转移、侵袭和血管生成,其作用机制可能是通过抑制PI3K/AKT信号通路实现的.

Objective:To explore the significance of bioresistance analysis on the clinical efficacy of PGLA embedding combined with Yiyi Umbilical needle in the treatment of abdominal obesity. Methods:A total of 90 patients with abdominal obesity admitted to our hospital from January 2020 to December 2021 were selected and randomly divided into 3 groups according to the ratio of 1∶1∶1, with 30 patients in each group. The group treated with yiyi Umbilical needle was the umbilical needle group, the group treated with PGLA suture embedding was the suture embedding group, and the group treated with PGLA suture embedding combined with Yiyi umbilical needle was the combined group. Body mass index（BMI）, waist circumference and waist-to-hip ratio（WHR） were measured before and after treatment, and body fat percentage, water percentage and muscle percentage were analyzed by bioresistance assay, and the clinical efficacy of the three groups were compared. Results:The effective rate of the umbilical needle group, the embedding group and the combined group were 90.00%, 93.33% and 96.67%, respectively. Although the effective rate of the combined group was higher than that of the umbilical needle group and the embedding group, the overall effective rate of the three groups had no significant difference（P＞0.05）. After treatment, BMI, waist circumference and WHR of the three groups were significantly improved compared with those before treatment（P＜0.05）, and the combined group was superior to the umbilical needle group and the embedding group（P＜0.05）. After treatment, the body fat percentage, water percentage and muscle percentage of the three groups were significantly improved compared with those before treatment（P＜0.05）, and the combined group was superior to the umbilical needle group and the embedding group（P＜0.05）. Conclusion:Single or combined application of PGLA suture embedding and Yiyiyizi umbilical needle in the treatment of abdominal obesity has good clinical efficacy. Through bioelectrical impedance analysis and comparison of efficacy observation indicators, it is found that compared with single treatment, combined treatment can better improve BMI, waist circumference, WHR and body composition, which has clinical application value.

岭南火针通过借火助阳、温通经络、开门驱邪、以热引热等作用，尤其可以温热之法治疗各种热性疾病，做到引热外达、托热外出，达到“清”“消”的作用，使岭南火针疗法有较广泛的临床使用场景。岭南火针以点代灸，刺激轻柔，患者接受度较高，在“治未病”思想指导下，临床通过岭南火针点刺大椎穴、百会穴、足三里穴、四花穴防治慢性咽炎、慢性鼻炎、失眠、慢性疲劳等疾病或不适状态收效较佳。岭南火针疗法凝聚了岭南地区人民智慧与地域特色，既保有了中医外治法“简、便、效、廉”的优点，又有较好的岭南地区的民众基础，尤为适用于本地湿热气候下防治慢性疾病。

目的:研究分析温针灸治疗脾胃虚寒型胃脘痛的临床效果.方法:选取脾胃虚寒型胃脘痛患者78例为研究对象,随机分为对照组和观察组,每组各39例.对照组患者采用常规性的治疗方法,观察组患者使用温针灸进行治疗.对比两组患者临床治疗效果.结果:治疗结果显示,观察组患者治疗有效率为94.9％,对照组患者治疗有效率为糍9％,数据符合统计学差异(P＜0.05);且观察组患者治疗时间为20.5d(s=4.7),对照组患者治疗时间为32.8d(s=6.9),数据符合统计学差异(P＜0.05).结论:治疗脾胃虚寒型胃脘痛,相对于常规性的治疗方法,温针灸治疗脾胃虚寒型胃脘痛疗效确切,能有效改善患者胃痛症状,还可缩短患者治疗时间,减少口服西药的副作用,可推广应用.

目的:研究分析电针结合艾灸治疗宫颈癌术后尿潴留的临床效果.方法:60例宫颈癌术后出现尿潴留患者,随机分对照组和观察组,各30例.对照组采用常规治疗,观察组采用电针结合艾灸治疗.对比两组患者临床治疗效果.结果:经治疗后,观察组患者治疗总有效率为93.3％,对照组患者治疗总有效率为63.3％,差异有统计学意义(P＜0.05);治疗前,两组患者残余尿量比较,差异无统计学意义(P＞0.05);治疗后,观察组优于对照组,差异有统计学意义(P＜0.05).结论:对于宫颈癌术后出现尿潴留的患者,采用电针结合艾灸治疗方法,能有效提高临床疗效,同时可有效减少患者残余尿量,有利于患者术后膀胱功能的恢复,提高患者的生活质量,在临床上具有指导应用价值.

Objective To investigate the influence of electroacupuncture and acupoint thread-embedding on glucose and lipid metabolism in abdominal obesity patients.Method A hundred and fifty patients with abdominal obesity were randomized into an electroacupuncture group,a thread-embedding group,and a combined group,50 each,receiving electroacupuncture treatment,acupoint thread-embedding treatment,electroacupuncture combined with acupoint thread-embedding treatment respectively.Waist circumstance,body weight,body mass index(BMI),glucose and lipid metabolism were compared among the three groups before and after treatment.Result The three groups all had significant changes in waist circumstance,body weight,and BMI score after treatment(P<0.05,P<0.01).Compared to electroacupuncture and thread-embedding groups,the combined group had marked differences in body weight and BMI score after treatment(P<0.05,P<0.01).The combined group had significant changes in fasting plasma glucose(FPG),fasting insulin(FINS),insulin sensitivity index(ISI),and insulin resistance index(IRI) after treatment(P< 0.01).Compared to electroacupuncture and thread-embedding groups,the combined group had significant differences in comparing ISI and IRI(P<0.01).The combined group and electroacupuncture group had significant changes in plasma cholesterol(CH),triglyceride(TG),low density lipoprotein(LDL),and high density lipoprotein(HDL) levels after treatment(P<0.05,P<0.01);the thread-embedding group had marked changes in CH,LDL,and HDL levels(P < 0.05,P < 0.01).Compared to the electroacupuncture group,the combined group and thread-embedding group both had significant differences in comparing HDL level after treatment(P<0.05,P<0.01).Conclusion Electroacupuncture,acupoint thread-embedding,and electroacupuncture plus thread-embedding are all effective in treating abdominal obesity,while the combined treatment can produce better therapeutic efficacy compared to the therapies used alone.