为了能探索贵阳不同林地下,种植种类不同比例的牧草对放养鸡的生长性能的作用,本项目组贵阳市贵州农业职业学院百宜基地的马尾松林下研究不同白三叶、黑麦草组合对林下养兴义矮脚鸡的饲养效果与养鸡对养殖场地植被生态的影响.马尾松林分为3个区域,种植不同比例的白三叶、黑麦草,选择90只兴义矮脚鸡平均放养到这3个区域.研究结果:林下放养模式下,鸡的放养活动有可能增加养殖场地的物种丰富度;兴义矮脚鸡的平均体重试验2组显著低于其他两组;试验2组、3组的平均采食量显著低于试验1组,综合来看试验3组的白三叶80％+黑麦草20％牧草组合可用于兴义矮脚鸡林下养殖.

平坝灰鹅是贵州省传统生产和消费的一个地方特色优良鹅种.针对制约平坝灰鹅产业发展的产业化程度低、未实现全年均衡生产、技术技能人才紧缺、品种资源保护和开发利用工作滞后等瓶颈问题,提出加强龙头企业培育、鹅产业化关键技术研究和推广、人才培养、品种资源保护和开发利用、品牌建设与推广等解决对策,为推进平坝灰鹅产业快速发展助力乡村振兴战略实施和种业振兴行动提供参考.

文章旨在研究日粮中添加不同蛋白质水平的苜蓿草粉对马岗雏鹅生长性能、血液生化指标和免疫机能的影响,试验选用210只马岗雏鹅,随机分为7组,每组3个重复,每个重复10只.对照组饲喂基础日粮,试验组分别在基础日粮基础上添加蛋白质含量为14％或16％的苜蓿草粉10％、20％和30％.结果表明,(1)日粮中添加苜蓿草粉对雏鹅日增重无显著影响,但试验III、V和VI组雏鹅平均日采食量显著高于对照组(P<0.05).(2)随着苜蓿草粉添加比例的升高,雏鹅血液中的尿酸、尿素和甘油三酯有下降趋势,试验I和试验IV组血糖和总胆固醇最大,且显著高于对照组(P<0.05).(3)各试验组IgA、IgM和IgG含量基本低于对照组.(4)日粮中苜蓿草粉的适宜添加量为10％～20％.

狮头鹅粉肝是潮汕人民因地制宜发明的特色美食.粉肝的生产是在约 1 d的时间内,用大米饭密集填饲 120 日龄左右的狮头鹅,使其肝脏重量迅速增长 3～4 倍至约 450 g.新鲜粉肝卤制后咸香嫩滑不油腻,备受消费者喜爱,同时粉肝生产亦为鹅产业带来了产值增加,促使粉肝产业展现强劲发展势头.本文通过实地调研狮头鹅粉肝生产情况,总结梳理了粉肝产业发展现状、生产工艺,并分析了其经济效益,旨在为粉肝产业的推广提供理论和经济学指导.

骨形态发生蛋白15(BMP15)作为转化生长因子-β超家族成员之一,该基因由2个外显子和1个较长的内含子组成,近几年研究发现,该基因对鸡生产性能有着重要影响.主要介绍了BMP15基因的结构,并归纳总结BMP15基因对鸡卵泡发育、基因多态性、体尺等鸡生产性能影响的研究进展,以期为研究BMP15基因对鸡生产性能的影响方面提供参考.

To investigate the effects of growth differentiation factor 9 (GDF9) and follistatin (FST) on porcine oocyte maturation and early embryo developmental competence,different concentrations of exogenous GDF9, anti-GDF9 antibody, FST and anti-FST antibody were added into in vitro maturation medium,and the nuclear maturation, cleavage, blastocyst rate and blastocyst cell number were detected. GDF9 significantly improved blastocyst rate. Anti-GDF9 antibody significantly inhibited cleavage and blastocyst rates. FST addition reduced parthenogenic embryo blastocyst rate and the total cell number of blastocyst. Maturation medium containing anti-FST antibody significantly improved blastocyst rates. Maturation medium containing GDF9 and anti-FST antibody together drove parthenogenic embryo blastocyst rate to the highest level. The results suggested GDF9 addition in maturation medium could improve oocyte maturation and early embryo developmental competence. GDF9 and anti-FST antibody in maturation medium could synergistically improve oocyte developmental competence.

This study was conducted to investigate the ovine key sterol-regulatroy genes expression from corpora lutea and different-sized follicles in the later luteal phase.10cast ages Hu sheep of proven fertility were used.After estrus synchronization,all ewes were slaughtered on day 12of estrous cycle,and corpora lutea and different-sized follicles were collected at the same time.The gene expression was determined by real-time PCR method.Compared with follicles≤ 2.5mm in diameter,the follicular very low density lipoprotein receptor(VLDLR),cytochrome P450(CYP17A1)and cytochrome P450aromatase(CYP19A1)mRNA expression were significantly increased(P0.05),however,follicular estrogen receptor 2(ESR2)mRNA expression was significantly decreased(P0.01).There were no significant effects of follicle size on follicular steroidogenic acute regulatory protein(STAR),cytochrome P450scc(CYP11A1),FSH receptor(FSHR),LH receptor(LHR),estrogen receptor 1(ESR1),low-density lipoprotein receptor(LDLR)and scavenger receptor-BI(SR-BI)mRNA expression,however,ESR1(P= 0.090)and SR-BI(P=0.093)expression trended increase in follicles2.5mm.CYP19A1and CYP17A1 were mainly,as expected,expressed in follicles,however,STAR,CYP11A1, FSHR,ESR2,LHR,ESR1,LDLR,SR-BI and VLDLRgenes were preferentially expressed in corpora lutea.In conclusion,sterol-regulatory genes took part in mediating follicular growth and progesterone synthesis of corpora lutea.

In order to obtain recombinant GDF9(growth differentiation factor 9) protein and anti-GDF9 antibody,a pair of primers designed according to the buffalo GDF9 gene mature peptide sequence(GenBank:FJ529501.1) was used to amplify the cDNA sequence of water buffalo GDF9 mature peptide with water buffalo genomic DNA as the template,and the alignment was made between the cDNA sequence and this region of other ruminant.The cDNA sequence was cloned into the BamH Ⅰ and Hind Ⅲsites of plasmid pRSET A to generate the expression vector pR-GDF9,which was further transformed into bacteria Escherichia.coli BL21(DE3).The transformed bacteria were induced to produce a recombinant GDF9 protein by IPTG.Then,the recombinant GDF9 protein was purified by 50% Ni-NTA agrose chromatography and homogenized into mineral oil adjuvant.The mixer was used to immunize New Zealand White rabbits to raise anti-GDF9 antibody.The titer of the antiserum was determined by ELISA.The results showed that buffalo GDF9 gene mature peptide sequence which shared high homology with other ruminant molecules was cloned successfully.E.coli BL21(DE3) expressing GDF9 protein with the expected molecular mass of 1.96×104 was obtained,and the highest expression level achieved 30% of total bacterial protein.Anti-GDF9 serum with titre of 1∶51 200 was obtained successfully and further purified to get high-purity anti-GDF9 antibody.The recombinant GDF9 protein prepared in this study can be used for immunizing sheep to improve ovulation rate and reproductive performance,and the anti-GDF9 antibody can be applied for improving animal embryo development in vitro.