胎盘部位滋养细胞肿瘤(PSTT)是来源于胎盘种植部位的一种特殊类型的滋养细胞疾病,临床罕见.PSTT病因尚不明确,可继发于流产、早产、足月产、葡萄胎等,因缺乏特异性临床表现及诊断金标准,常致漏诊、误诊.现结合文献复习,总结1例PSTT的疾病特点.

Cervical cancer is a common public health issue with high morbidity worldwide. Paeonol (Pae) has been recognized as a traditional Chinese medicine used for the treatment of various cancer types. However, whether Pae could exert a protective effect on cervical cancer remains to be investigated. The aim of the present study was to explore the role of Pae in cervical cancer cells and identify the potential mechanism. Cell Counting Kit-8 and colony-formation assays were conducted to test the proliferation of HeLa cells. Additionally, wound healing and transwell assays were used to detect the migratory and invasive abilities of cells. The plasmid that overexpressed 5-lipoxygenase (5-LO) or control vector was constructed and transfected into the cells. Subsequently, flow cytometry was used to monitor the apoptotic rate of cells. The expression levels of apoptosis-associated proteins and 5-LO were detected using western blot analysis. Reverse transcription-quantitative PCR analysis detected the expression of 5-LO. Pae inhibited the proliferation, invasion and migration of HeLa cells, promoted cell apoptosis and downregulated the expression of 5-LO. Overexpression of 5-LO, however, attenuated these effects. Thus, Pae could inhibit the proliferation, migration and invasion, as well as promote apoptosis of HeLa cells by regulating the expression of 5-LO.

[目的]观察三七重楼生化汤(Sanqi Chonglou Shenghua Decoction,SCD)对L929成纤维细胞增殖、黏附、迁移及愈合相关蛋白表达的影响,并探讨其机制.[方法]常规培养L929成纤维细胞,随机分为SCD低、中、高浓度组及对照组,SCD各浓度组予不同浓度SCD(1.25、2.5、5mg·mL-1),对照组予等体积培养基,以实时细胞分析技术(real time cellular analysis,RTCA)、细胞增殖检测试剂盒(cell counting kit-8,CCK-8)分别检测细胞增殖、黏附能力,划痕实验检测细胞迁移情况,Western blot检测血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)、转化生长因子-β1(transforming growth factor-β1,TGF-β1)、细胞外调节蛋白激酶1/2(extracellular regulated protein kinases 1/2,ERK1/2)、磷酸化细胞外调节蛋白激酶1/2(phospho-extracellular regulated protein kinases 1/2,p-ERK1/2)蛋白表达.[结果]SCD促进成纤维细胞增殖,且呈动态变化.RTCA示:SCD干预12～24h,中、高浓度促进细胞增殖(P<0.01),但组间比较,差异无统计学意义(P>0.05).CCK-8结果示:SCD干预24h,低、中、高浓度组均促进细胞增殖(P<0.01).与低浓度组比较,中、高浓度组促增殖作用更显著(P<0.01).与中浓度组比较,高浓度组促增殖作用更显著(P<0.05).SCD促进成纤维细胞迁移.干预24h,中、高浓度组促进细胞迁移(P<0.05,P<0.01),且高浓度组促进作用更显著(P<0.01).干预36h,低、中、高浓度SCD均具有促进迁移的作用(P<0.05,P<0.01).与低浓度组比较,中、高浓度促进作用更显著(P<0.05,P<0.01).与中浓度组比较,高浓度组促进迁移作用更显著(P<0.01).SCD促进成纤维细胞黏附(P<0.01),但不同浓度间差异无统计学意义(P>0.05).SCD高浓度组可上调成纤维细胞VEGF、p-ERK1/2的表达(P<0.01),但SCD不影响TGF-β1表达(P>0.05).[结论]SCD可通过促进成纤维细胞增殖、黏附和迁移,并促进VEGF蛋白表达,从而发挥促进剖宫产瘢痕愈合的作用,其机制可能与ERK信号通路激活有关.

目的:探讨电针、中药二法联合盆底肌训练治疗围绝经期妇女压力性尿失禁(stress urinary incontinence,SUI)的疗效.方法:选择2016年10月至2017年9月在浙江中医药大学附属第二医院围绝经期门诊确诊的轻中度SUI患者178例,最终有162例(对照组54例,治疗组152例,治疗组256例)患者完成治疗及随访.对照组给予Kegel运动训练,治疗组1在对照组的基础上给予电刺激生物反馈治疗,治疗组2进行中医辨证分型后在对照组的基础上给予补中益气汤加减及电针的综合治疗,三组均治疗2个疗程并定期随访6个月;于治疗结束时、结束后3、6个月行1h尿垫试验、填写国际尿失禁咨询委员会尿失禁问卷简表(ICI-Q-SF),于治疗结束时、结束后6个月行尿动力学检测,并进行疗效评价.结果:三组治疗结束时、结束后3、6个月1h尿垫试验尿漏量、ICI-Q-SF评分较治疗前明显减少(P<0.05);治疗结束时、结束后6个月腹压漏尿点压力(AL-PP)、最大尿道闭合压(MUCP)、膀胱顺应性(BC)较治疗前明显增加(P<0.05);治疗组1、2上述指标改善情况均显著优于对照组(P均<0.05);而治疗组2在1h尿垫试验尿漏量上的疗效更优于治疗组1(P<0.05),在ICI-Q-SF评分,各项尿动力学指标上的改善与治疗组1无统计学差异(P>0.05).结论:通过电针、中药二法联合盆底肌训练对于治疗轻中度围绝经期妇女SUI,能减少尿漏量,增加ALPP、MUCP,提高BC,明显改善患者生活质量.