Background Osteosarcoma (OS) has been the most common malignancy of the bone in children and adolescents, and the unsatisfactory prognosis of OS sufferers has long been a hard nut. Here, we delved into the markers with a prognostic value for predicting the prognosis of OS patients. Methods The messenger RNA (mRNA) sequencing data and clinical data of OS were retrieved from a Gene Expression Omnibus (GEO) dataset (GSE39058). Next, prognosis-related genes (PRGs) were filtered with the aid of Kaplan–Meier (K-M) curves and Cox regression analysis (CRA). Later, Gene Ontology (GO) biological process analysis was used in verifying the function of different genes. CCK-8 and cell apoptosis assay were performed to evaluate the function of MFNG in U2OS cells. Results Among the obtained genes, Manic Fringe ( MFNG ) had the closest relevance to prognosis and clinical traits, thus becoming the research object herein. In light of the expression level of MFNG , patients fell into high- and low- MFNG groups. Patients with elevated MFNG expression had a worse prognosis, according to the survival analysis. It was unveiled by the univariate and multivariate analyses that MFNG expression was an independent adverse prognostic factor for disease-free survival in OS patients (p = 0.006). Meanwhile, MFNG expression was linked to gender and tumor recurrence, and it was higher in patients with OS recurrence. Moreover, overexpression of MFNG promoted the cell proliferation and inhibited the cell apoptosis of U2OS cells. Conclusions The expression level of MFNG negatively correlated with OS progression, and as an independent adverse prognostic factor for disease-free survival in OS patients. Moreover, MFNG regulated the cell proliferation and apoptosis of OS cells.

膝关节自发性复发性出血非常罕见,主要发生于合并骨关节炎的高龄人群.这类血肿最早由Wilson[1]报道,其认为滑膜炎症是出血的原因,因此滑膜切除术即是最合理的治疗方案[2?3].然而,Kawamura等[4]在1994年报道了5例伴有外侧半月板后角退变性撕裂的膝关节自发性出血病人,在经过关节镜下撕裂半月板切除术后,这5例病人均未出现复发,从而证实,膝关节自发性复发性出血来源于外侧半月板后角的周围动脉.此后,又有一些报道证实了这一理论[5?8],其中Sasho等[7]在切除半月板的过程中还探查到了搏动的动脉出血,考虑为膝外侧动脉的分支.但作为出血来源,其动脉本身结构从未被发现过.我们于2019年5月收治了一例膝关节自发性复发性出血病人,并在关节镜术中于关节囊的后外侧角发现了一个管状的搏动性出血结构,亦在内侧半月板前角发现带血管的滑膜攀附,现报告如下.

BACKGROUND: At present, minimally invasive total hip arthroplasty is gradually developed, and becomes one of the representative types of total hip arthroplasty. The direct anterior approach is characterized by small tissue injury, low postoperative complications and rapid postoperative recovery. Compared with other approaches, there is still a lot of controversy on whether the approach can obtain better early and long-term effects. OBJECTIVE: To compare the clinical effect of direct anterior approach and posterolateral approach in minimally invasive total hip arthroplasty. METHODS: Totally 88 patients with minimally invasive total hip arthroplasty from September 2015 to September 2016 in the First Department of Orthopedics, Changzhou City Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Traditional Chinese Medicine were selected as the subjects. The patients were divided into direct anterior approach group and posterolateral approach group, with 44 patients in each group. The incision length, operation time, postoperative drainage volume, blood transfusion volume, hemoglobin difference before and after operation, time of bed walking, postoperative acetabular abduction angle and acetabular anteversion angle were recorded and compared. Blood sedimentation rate, C-reactive protein levels, complications (infection, vascular injury and hip dislocation), hip function Harris score preoperatively, postoperatively 1, 3, and 6 months were analyzed. RESULTS AND CONCLUSION: (1) There was no significant difference in the postoperative acetabular abduction angle and acetabular anteversion angle between both groups (P > 0.05). (2) The average operation time of the patients in the direct anterior approach group was significantly longer than that in the posterolateral approach group (P < 0.05). The length of incision, intraoperative blood loss, postoperative drainage volume, hemoglobin difference, postoperative erythrocyte sedimentation rate, postoperative C-reactive protein and time of bed walking were significantly lower in direct anterior approach group than in posterolateral approach group (P < 0.05). (3) Harris score of direct anterior approach group was significantly higher than that of posterolateral approach group (P < 0.05). There was no significant difference in Harris score between the two groups before and 6 months after operation (P > 0.05). (4) There was no significant difference in postoperative complications between the two groups (P > 0.05). (5) There is no significant difference in the efficacy during 6-month follow-up and postoperative complications between posterolateral approach and direct anterior approach, but the direct anterior approach is less traumatic, with faster postoperative recovery, higher joint stability, more consistent with minimally invasive surgery and the concept of rapid rehabilitation.

Objective To investigate the incidence of osteopenia and osteoporosis in oldermen selected for cementless total hip arthroplasty (THA). Methods A subgroup of fifty-two male patients (average age, 64.7 years) with advanced primary hip OA scheduled for cementless THA were recruited for DXA and laboratory screening. Before surgery, bone mineral density (BMD) of the lumbar spine, the proximal femurs and the distal forearm were measured. The serum concentrations of calcium, 25 - hydroxyvitamin D, parathyroid hormone and biochemical markers of boneresorption and formation were determined to exclude secondary OP. Results The prevalence of OP (T score < 2.5) and osteopenia (1.0 > T score > 2.5) were 29% and 44%, respectively. Statistically, OP was related to patient's age, and 4 patients (8%) had abnormal laboratory findings. One was found to have a parathyroid adenoma. The prevalence of vitamin D insufficiency [S 25 (OH) D levels ≤ 50 nmol/l] was 37%( = 19). As a sign of high bone turnover, the patients with reduced BMD values showed significantly increased serum levels of osteocalcin ( =0.001), intact procollagen type I N propeptide ( =0.010) and N-terminal crosslinking telopeptide of type I collagen ( = 0.048). The BMC of the femoral necks of the osteoarthritic hips were significantly higher ( = 0.000) and the BMC of the trochanter regions were significantly lower ( = 0.005) compared to the contralateral hips. Conclusions The majority (73%) of the male hipOA patients are osteopenic or osteoporotic with signs of increased bone turnover.An unexpectedly high number of patients require preoperative consultation with anendocrinologist. The altered distribution of BMD observed in the proximal femurs may explain the lower prevalence of fractures reported in the femoral necks of osteoarthritic hips.

Sirtuin1 (SIRT1) has protective effects in some neurodegenerative disease models, but it is not clear whether SIRT1 play the same role on inflammation-mediated Parkinson's disease (PD) models. In this study, we firstly established an inflammation environment by stimulating microglial BV-2 cells with the inflammatory agent lipopolysaccharides (LPS), which demonstrated by increasing of the levels of TNF-a, and IL-6 in cultured medium. Then we exposed PC12 cells (a model of catecholaminergic neuronal cells) with the supernant from LPS stimulated BV-2 cells (activated BV-2). PC12 cell apoptosis and SIRT1 involved protection were investigated. The results indicated that treatment with LPS caused significant decrease in SIRT1 expression in activated BV-2 cells, and increased the levels of TNF-a and IL-6, as measured by ELISA, whereas resveratrol (a known SIRT1 activator) suppressed this effect, which was conversely strengthened by sirtinol (a SIRT1 inhibitor), suggesting that SIRT1 may be involved in regulating proinflammatory cytokines from microglial activation. Further, we found that factors derived from activated microglia significantly decreased the level of deacetylation of p53 by reducing the expression of SIRT1, an effect that increased the apoptosis of PC12 and reduced cell viability. The addition of resveratrol could protect PC12 cells from inflammation-mediated damage above-mentioned, while nicotinamide (another SIRT1 inhibitor) treatment had the opposite effect of resveratrol. Together, these data suggests that: SIRT1 inhibits LPS-mediated proinflammatory cytokines release in microglia, and circumvents dopaminergic neurons injury induced by activated microglial-derived factors via p53-caspase-3-dependent mechanism of apoptosis. Thus, upregulation of SIRT1 provides a promising research field for therapeutic intervention in neuroinflammation diseases.