Aim of the study: Polymorphisms of DNA repair enzyme gene may alter the ability of damage repair, ischemic stroke susceptibility and outcome. This study aimed to explore the association of polymorphisms in PARP1 and the effects of interactions between genes in Chinese.Materials and methods: A total of 500 patients and 500 healthy controls were enrolled for genotyping. Results: Clinical information analysis revealed higher levels of alcohol and smoking exposure in patients with ischemic stroke, as well as chronic conditions such as diabetes, hypertension, and higher serum triglycerides concentration. In addition, Polymorphism in PARP1 rs8679 was significantly associated with the decreased ischemic stroke risk. Patients harboring the PARP1 rs8679 AG/GG genotype had a better initial stroke, and as for the mRNA level of PARP1, it was suppressed with mutant genotype in comparison with the wild genotype. Finally, the suppressed of PARP1 was induced by gain-binding ability of miR-124-5p through 3'UTR directly binding.Conclusions: In conclusion, our study demonstrates that the SNP rs8679 in PARP1 3 '-UTR might act as a protective factor for the outcome of patients with ischemic stroke.
OBJECTIVE:To determine the effect of PARP1 polymorphism on gene interactions.METHODS:A total of 500 patients and 500 healthy controls were enrolled.RESULTS:Analysis of clinical data showed that patients with stroke, diabetes, hypertension, and elevated serum triglyceride levels had higher levels of alcohol and smoking. The polymorphism of PARP1rs8679 was inversely associated with the risk of ischemic stroke. Patients with PARP1rs8679AG/ GG genotypes had a lower incidence of an initial stroke. Compared with the wild genotype, mRNA levels of PARP1 were reduced. MiR-124-5p directly induced PARP1 inhibition through the gain binding ability of 3 'UTR binding.CONCLUSION:Single nucleotide polymorphism (SNP) rs8679 in PARP13'UTR can prevent ischemic stroke.
目的 观察术后综合康复治疗对踝关节骨折患者康复进程及生活质量的影响.方法 将147例踝关节骨折病例随机分为观察组(n=76)和对照组(n=71).观察组术后给予综合康复治疗方案,对照组术后给予常规康复方案.随访至术后12个月,观察两组康复进程(采用踝关节主动伸、屈、旋前、旋后最大度数评价,术前及术后3,6,12个月各评价1次)及生活质量的差异.结果 两组踝关节伸、屈、旋前、旋后度数比较差异有统计学意义(P<0.05),两组踝关节伸、屈、旋前、旋后度数随着时间的延长表现出逐渐升高的趋势(P<0.05),观察组升高的幅度大于对照组(P<0.05);两组的躯体功能、心理功能、社会功能、物质生活状态评分比较差异有统计学意义(P<0.05),且评分随着时间的延长表现出逐渐升高的趋势(P<0.05),观察组升高的幅度大于对照组(P<0.05).结论 综合康复治疗可缩短踝关节骨折术后康复进程,也有助于提升生活质量.
