Aim of the study: Polymorphisms of DNA repair enzyme gene may alter the ability of damage repair, ischemic stroke susceptibility and outcome. This study aimed to explore the association of polymorphisms in PARP1 and the effects of interactions between genes in Chinese.Materials and methods: A total of 500 patients and 500 healthy controls were enrolled for genotyping. Results: Clinical information analysis revealed higher levels of alcohol and smoking exposure in patients with ischemic stroke, as well as chronic conditions such as diabetes, hypertension, and higher serum triglycerides concentration. In addition, Polymorphism in PARP1 rs8679 was significantly associated with the decreased ischemic stroke risk. Patients harboring the PARP1 rs8679 AG/GG genotype had a better initial stroke, and as for the mRNA level of PARP1, it was suppressed with mutant genotype in comparison with the wild genotype. Finally, the suppressed of PARP1 was induced by gain-binding ability of miR-124-5p through 3'UTR directly binding.Conclusions: In conclusion, our study demonstrates that the SNP rs8679 in PARP1 3 '-UTR might act as a protective factor for the outcome of patients with ischemic stroke.

OBJECTIVE:To determine the effect of PARP1 polymorphism on gene interactions.METHODS:A total of 500 patients and 500 healthy controls were enrolled.RESULTS:Analysis of clinical data showed that patients with stroke, diabetes, hypertension, and elevated serum triglyceride levels had higher levels of alcohol and smoking. The polymorphism of PARP1rs8679 was inversely associated with the risk of ischemic stroke. Patients with PARP1rs8679AG/ GG genotypes had a lower incidence of an initial stroke. Compared with the wild genotype, mRNA levels of PARP1 were reduced. MiR-124-5p directly induced PARP1 inhibition through the gain binding ability of 3 'UTR binding.CONCLUSION:Single nucleotide polymorphism (SNP) rs8679 in PARP13'UTR can prevent ischemic stroke.

目的 研究脑卒中患者应用区域三级康复网络服务后的临床效果.方法 回顾性分析2017年1月至2018年12月江阴市第五人民医院收治的90例脑卒中患者,依据采取的康复治疗方法不同,分为研究组与对照组,各45例.研究组应用区域三级康复网络服务,对照组选取常规治疗.比较两组简化Fugl-Meyer运动功能量表评分、改良Barhel指数量表评分.结果 研究组在进入一级康复治疗15?d时、转入二级康复治疗90?d时、转入三级社区康复治疗60?d时的简化Fugl-Meyer运动功能量表评分、改良Barhel指数量表评分均显著高于对照组(P<0.05).结论 建立具有统一转诊流程以及康复治疗方案、完备患者信息数字网络平台,统一康复医师技能培训的区域三级康复网络,能够有效改善脑卒中患者的肢体运动功能和日常生活活动能力.

目的:评价江阴市三级康复网络的脑卒中康复治疗效果.方法:选取江阴市人民医院和第五人民医院2016年1月到2017年5月收治的240例的脑卒中患者.随机分为双向转诊组(n=120)和对照组(n=120).双向转诊组通过三级康复网络双向转诊完成康复治疗;对照组仅在一家医院完成康复治疗.比较分析两组患者住院时间、治疗费用、改良Barthel指数(MBI)、Fugl-Meyer运动功能量表(FMMS).结果:双向转诊组住院时间比对照组显著增加(P<0.05),总治疗费用显著降低(P<0.05).双向转诊组康复治疗后MBI及FMMS评分,康复治疗后60天和90天MBI及FMMS评分显著高于同期对照组(P<0.05).年龄、发病类型、是否双向转诊、住院时间、FMMS评分是康复治疗后生活能力的独立影响因素.结论:江阴市三级康复网络规范化的双向转诊有助于提高康复疗效并降低治疗费用.

目的 探究奥塔戈运动训练应用于老年中风后运动功能障碍中的效果.方法 选取老年中风后运动功能障碍患者88例,按照时间顺序分别设为对照组和观察组,分别应用常规训练和奥塔戈运动训练.对比两组平衡力、步行能力及负性情绪.结果 观察组干预3个月后平衡力高于对照组(P<0.05);观察组干预3个月后步行能力优于对照组(P<0.05);观察组干预3个月后SAS、SDS评分均低于对照组(P<0.05).结论 奥塔戈运动训练应用于老年中风后运动功能障碍患者中,可显著增强患者平衡力,提高其步行能力,并有效缓解其负性情绪.

目的 探讨中药内服外熏联合手法整复治疗Colles'骨折的临床效果及对腕部关节功能恢复的影响.方法 收集Co11es'骨折患者72例,分为实验组和对照组,各36例,对照组接受手法整复治疗,实验组在对照组基础上服用中药桃红四物汤和接受本院自制中药剂熏洗.评估两组临床疗效、骨折愈合情况及腕部关节功能恢复效果.结果 实验组临床治疗总有效率高于对照组(P<0. 05).实验组治疗4周、6周后腕关节损伤评分均明显低于对照组(P<0. 05),且骨折愈合时间短于对照组(P<0. 05).两组治疗4周后腕部关节功能恢复效果比较无统计学意义(P>0. 05),治疗6周后背伸、掌屈、桡偏、尺偏、旋前、旋后角度均明显増高(P<0. 05),而实验组背伸、掌屈、桡偏、尺偏、旋前、旋后角度增高幅度明显大于对照组(P<O. 05).治疗6周后实验组腕关节功能优良率高于对照组(P<0. 05).结论 手法整复基础上联合中药内服外熏治疗Colles'骨折可显著提高临床疗效,并有效促进骨折愈合和腕部关节功能恢复.

Single-nucleotide polymorphisms (SNPs) play an important role in our susceptibility to disease, the severity of illness and the way our body responds to treatment. This study evaluated the impact of three polymorphisms on the susceptibility and functional outcome of ischemic stroke (IS). Three hundred and eight patients and 300 healthy volunteers were enrolled. Polymorphisms of NOX4 rs11018628, MTHFR rs1801133 and NEIL3 rs12645561 were detected in both groups. Smoking (P<0.001), drinking (P<0.001), hypertension (P<0.001) and diabetes (P=0.006), as traditional vascular risk factors for IS, were confirmed in our study. Logistic regression analyses with adjustment for age, sex, smoking, drinking, diabetes, hypertension and total cholesterol showed that the variant genotypes of NOX4 rs11018628 were associated with a significantly decreased risk (Dominant model: OR=0.32, 95% CI=0.22-0.48, P<0.001) and a better short-term recovery of IS (Dominant model: OR=0.57, 95% CI=0.35-0.95, P=0.029). This study demonstrates that the NOX4 rs11018628 SNP is associated with decreased risk in developing IS and better short-term recovery of patients. This suggests that the genetic variant of NOX4 rs11018628 may contribute to the etiology of IS.

目的:探讨联用中药外敷疗法和手法复位治疗四肢骨折的临床效果.方法:选择2017年3月至2018年5月期间江阴市第五人民医院中医骨伤科收治的100例四肢骨折患者作为研究对象.将这100例患者分为对照组和研究组.对两组患者均进行手法复位.在此基础上,为对照组患者使用洛索洛芬钠片进行治疗,为研究组患者使用中药外敷疗法进行治疗.然后,比较两组患者治疗前后其VAS(疼痛视觉模拟评分法)的评分、Gartland-Werley(腕关节缺陷评分系统)的评分、Kofoed(踝关节功能评分标准)的评分、疼痛的缓解率、治疗的总有效率及不良反应的发生率.结果:治疗后,与对照组患者相比,研究组患者治疗的总有效率更高,其不良反应的发生率更低(P<0.05).治疗7 d后,与对照组患者相比,研究组患者疼痛的缓解率更高,其VAS的评分更低(P<0.05).治疗后2个月,与对照组患者相比,研究组患者Gartland-Werley、Kofoed的评分均更高(P<0.05).结论:联用中药外敷疗法和手法复位治疗四肢骨折的临床效果较好,可有效地缓解其疼痛,降低其不良反应的发生率,并有利于其关节功能的恢复.

Background/Aims: Genetic polymorphisms of methylene tetrahydrofolate reductase (MTHFR) were associated with ischemic stroke risk. This study analyzed MTHFR polymorphisms at the 3'-untranslated region for association with risk and outcome of ischemic stroke in a Chinese Han population. Methods: 500 patients and 600 healthy volunteers were enrolled for MTHFR rs868014 genotyping identified bioinformatically. The binding of miR-1203 to MTHFR rs868014 was determined by luciferase assay, MTHFR expression was assessed using qRT-PCR, and plasma homocysteine levels were assayed by ELISA. Results: Cigarette smoking, alcohol consumption, diabetes, hypertension (all P <0.001), low levels of serum high-density lipoprotein-C (P = 0.01), and high levels of serum low-density lipoprotein-C (P = 0.005) were associated with an increased risk of developing ischemic stroke. BMI and total serum cholesterol concentration was not associated with ischemic stroke. MTHFR rs868014 TC and CC genotypes were significantly associated with increased risk of ischemic stroke compared with the TT genotype (OR: 1.52; 95% CI: 1.01-3.39 for TC genotype, while OR: 1.99; 95% CI: 1.29-3.88 for CC genotype). Furthermore, the MTHFR rs868014 SNP was associated with a poor short-term ischemic stroke outcome. qRT-PCR confirmed that MTHFR rs868014 TC or CC genotypes could facilitate miR-1203 binding leading to low MTHFR levels in cells. In addition, patients carrying the MTHFR rs868014 TC or CC genotypes were associated with accumulation of serum tHcy and a poor ischemic stroke outcome. Linkage disequilibrium analysis indicated that the newly identified SNP rs868014 was strongly linked with the MTHFR A1298C polymorphism. Conclusion: This study demonstrates that the MTHFR rs868014 SNP is associated with increased risk in developing ischemic stroke, miR-1203 binding, low MTHFR levels in cells, and poor shot term outcome of patients.

目的 观察术后综合康复治疗对踝关节骨折患者康复进程及生活质量的影响.方法 将147例踝关节骨折病例随机分为观察组(n=76)和对照组(n=71).观察组术后给予综合康复治疗方案,对照组术后给予常规康复方案.随访至术后12个月,观察两组康复进程(采用踝关节主动伸、屈、旋前、旋后最大度数评价,术前及术后3,6,12个月各评价1次)及生活质量的差异.结果 两组踝关节伸、屈、旋前、旋后度数比较差异有统计学意义(P<0.05),两组踝关节伸、屈、旋前、旋后度数随着时间的延长表现出逐渐升高的趋势(P<0.05),观察组升高的幅度大于对照组(P<0.05);两组的躯体功能、心理功能、社会功能、物质生活状态评分比较差异有统计学意义(P<0.05),且评分随着时间的延长表现出逐渐升高的趋势(P<0.05),观察组升高的幅度大于对照组(P<0.05).结论 综合康复治疗可缩短踝关节骨折术后康复进程,也有助于提升生活质量.

目的：观察独活寄生汤加减熏蒸治疗膝骨性关节炎的临床疗效。方法入选82例膝骨性关节炎患者随机分为对照组和治疗组。对照组41例患者给予口服盐酸氨基葡萄糖胶囊0.75 g bid；治疗组41例患者在口服盐酸氨基葡萄糖胶囊基础上给予膝部独活寄生汤加减熏蒸治疗，1次/d。观察30 d后两组患者的疗效。结果治疗组总有效率明显高于对照组(<0.05)。两组患者不良反应发生率没有统计学差异(>0.05)。结论采用独活寄生汤加减熏蒸治疗膝骨性关节炎，不仅方法简单，且具有显著的疗效，副作用较小，值得临床上推广应用。

Objective To explore the curative effect and safety of transplanting autologous mesenchymal stem cells to patients with Parkinson's disease.Methods Forty-two patients with Parkinson's disease were selected and randomly divided into a control group and a research group,each of 21.Both groups were given routine treatment and rehabilitation,but the research group was additionally provided with autologous bone marrow mesenchymal stem cell transplantation.Bone marrow mesenchymal stem cells were isolated from the patients,cultured and transplanted back into the patients totally 4 times at intervals of 5 to 10 days.Before the treatment and 4 weeks and 3 months later,the clinical functioning of both groups was evaluated using the Unified Parkinson's Disease Rating Scale (UPDRS).Four weeks and 3 months after the treatment,the peripheral blood expression of vascular endothelial growth factor (VEGF),the expression of interleukin 10 (IL-10) and the expression of CSF tumor necrosis factor alpha (TNF-α) in cerebrospinal fluid were compared between the 2 groups.Results The average UPDRS score of the research group decreased from (41.26± 17.92) at four weeks to (33.67± 17.77) at 3 months after the treatment,both significantly lower than before the treatment,and significantly lower than the scores of the control group at the same time points [(47.13±18.35) and (39.03±16.50)].During the treatment,no severe adverse reactions were observed among the research group.Moreover,after the treatment the blood expression of VEGF and the expression of IL-10 in cerebrospinal fluid were significantly improved in the research group,while that of TNF-α was significantly reduced compared to before the treatment.nclusions The transplantation of autologous mesenchymal stem cells can safely promote nerve function in Parkinson's disease patients.It is worth applying in clinical practice.

Objective To explore a set of service network management system for blood pressure, which is suitable for hospital- community- service station, and analyze the effect in the improvement of management quality of prevention, control and treatment, and reducing of the incidence and mortality of hypertension. Methods The hospital- community- service was connected through the computer technology and internet technology, formed to be a powerful blood pressure management network and database. 500 cases of hypertension patients who were admitted in the Fifth People's Hospital of Jiangyin City( hospital for short) from January 2010 to June 2013 were included in the computer management system, whose basic information, medical history, clinical manifestation, examination, complications, as well as diet, exercise, drugs treatment and some other information were recorded in the system. Regular medication rate, blood pressure prevention and control knowledge awareness, treatment effectiveness, blood pressure control of all the objects before and after the application of computer system were compared. Results The regular medication rate, blood pressure prevention and control knowledge awareness, treatment effectiveness, blood pressure control rates increased after the application of computer system(92%, 94%, 89%, 91%) were significantly higher than those before the application of computer system(63%, 77%, 77%, 66%), the differences were statistically significant(P 0.05). Conclusion Taking the advantages of computer technology and combinating with hypertension management, using the computer management system to manage the hypertension patients and establish a set of high blood pressure and health archives for each blood pressure patient can provide a new way of networking, informatization and standardization management for hypertension in our country.