It was conducted the investigation of secondary metabolites by gas chromatography-mass spectral analysis of benzene extracts of the aerial parts of Artemisia sogdiana Bunge. and Artemisia serotina Bunge., collected during budding and early flowering spurs Nurata ridge Jizzakh region of Uzbekistan. The basic components of A. sogdiana are 5,5-dimethyl-2(5Н)-furanone – 17,99%, g-vinyl-g-valerolactone – 8,71%; monoterpenoids: 1,8-cineole – 28,13%, a-thujone – 3,18%, b- thujone – 2,69%, camphor – 8,65%, filifolide А – 4,05%, chrysanthenone – 5,23%.The basic components of A. serotina are 1,8-cineole – 10,08%, filifolide А – 8,62%, chrysanthenone – 13,00%, (Z)-jasmone – 1,95%.The represented in this report A. sogdiana and A. serotina both have available α-pinene, 5,5-dimethyl-2(5Н)-furanone, g-vinyl-g-valerolactone, 1,8-cineole, chrysanthenone, α-isophorone and filifolide А, which could be marker compounds for them.It should also be noted that the detected volatile monoterpenoids α- and β-thujones, camphor and 1,8-cineole are active inhibitors of seed germination and growth of seedlings of annual plants surrounding.The analysis identified 19 and 22 compounds of those Artemisia species for the first time, respectively.