AbstractCold atmospheric‐pressure plasma jet not only modifies surface morphology but also introduces functional groups on sample surfaces. In this work, TiO2nanorods/carbon fiber (TNCF) is hydrothermally prepared, and hydrogen‐doped TNCF (H:TNCF) is obtained by the surface treatment of TNCF using cold atmospheric‐pressure Ar/H2Plasma jet. H and OH spectral lines are observed from the emitting spectrum of atmospheric‐pressure Ar/H2plasma jet. Nanoscaled protrusions appear at top of TiO2nanorods after the surface treatment of cold atmospheric‐pressure Ar/H2plasma jet. OH functional groups form on the surface of H:TNCF, based on the results of FTIR, Raman and XPS analysis. Furthermore, hydrogen doping can lower the band gap from 3.2 eV of TNCF to 2.9 eV of H:TNCF, and reduce the work function from 4.97 eV of TNCF to 4.71 eV of H:TNCF. Field emission property and cell compatibility of TNCF and H:TNCF are investigated. Field emission property of TNCF is considerably improved by hydrogen doping, due to a reduced work function and a higher field enhancement factor. In the cell attachment experiments, H:TNCF exhibits more attached cells with a larger cell attachment area, compared with the TNCF sample. The obtained results show H:TNCF prepared by hydrothermal method combined with cold atmospheric‐pressure Ar/H2Plasma jet will be a promising candidate for field emission and biocompatibility applications.
为研究AP-2α在发育过程中的特定调控作用,利用AP-2α-LacZ转基因小鼠FX18研究AP-2α与先天性心脏病的相关性.在FX18小鼠繁殖过程中,发现该小鼠稳定遗传的突变体,鉴定其基因型,测量体质量与心脏质量,HE染色和心电图观察其表型和检测心脏功能.研究结果表明:突变体小鼠在出生后4周左右即死亡,且在胚胎期就表现出明显的发育迟缓,出生后的体质量和心脏质量均仅为正常FX18小鼠的40％～60％,同时,HE染色和心电图检测结果显示,小鼠表现出较明显的左心室肥大和室性早搏,符合先天性心脏病中动脉导管未闭的特征.因此,成功发现和建立的该突变体小鼠可作为一个较好的研究先天性心脏病小鼠模型.
为了深入探讨乳腺癌治疗耐药的发生机制并寻找乳腺癌治疗耐药的潜在治疗手段,我们以乳腺癌MCF-7和耐阿霉素MCF-7/ADR细胞为研究对象,研究RNA甲基化酶WTAP对其迁移的影响.MTT试验发现阿霉素对MCF-7细胞活力的抑制作用大于对MCF-7/ADR细胞的抑制作用.qPCR和western-blot试验发现WTAP在耐阿霉素细胞MCF-7/ADR中高表达,同时transwell试验发现在MCF-7细胞中增加WTAP的表达对MCF-7细胞的迁移没有影响,而在MCF-7/ADR细胞中敲低WTAP的表达会抑制MCF-7/ADR细胞的迁移.western-blot试验进一步证明了这一作用是通过抑制上皮间质转化(EMT)来发挥的.这一发现有助于为乳腺癌的临床治疗提供新的理论依据并为乳腺癌的治疗提供新的策略.
