The different photoelectric signals in bacteriorhordopsin areexperimentally measured when it is excited by 10 ns pulsee lasers at 1064 nm, 532 nm and 355 nm. 532 nm pulse laser is used mostly as pumping light to measure the photoelectric signals affected by the impedance of the measuring circuit. The mechanism that bacteriorhodopsin generates photo-charges and the photo-charges transfer in it was studied and analyzed. The results show that the positive and negative photoelectric signals are produced by the shift of the positive and negative charges respectively, and more energy is needed to produce positive charges than negative ones. At the same time, the positive and negative charges not only have their own transfering characteristics, but also affect each other, such as, positive charges move slowly, and negative ones fast, moreover, the negative can pull the positive to move forward, which, together with the interface between the sample and electrodes, cause the magnitude and width of the pulse photo-voltage having special dependence different from other photo-electronic devices on the linked resistance.

The different photoelectric signals in bacteriorhodopsin were experimentally measured when it was excited by 10 ns pulsed lasers at 1064 nm, 532 nm and 355 nm. 532 nm pulsed laser is used mostly as pumping light to measure the photoelectric signals affected by the impedance of the measuring circuit. The mechanism that bacteriorhodopsin generates photocharge and the photocharge transfer in it was studied and analyzed. The results show that the positive and negative photoelectric signals are produced by the shift of the positive and negative charge respectively, and more energy is needed to produce positive charge than negative charge. The positive and negative charge not only have their own transfer characteristics but also affect each other, such as positive charge moves slowly and negative charge moves fast, while the negative charge can pull the positive charge to move forward. These characteristics are related to the interfacial effect between the sample and electrodes so that the magnitude and width of the pulse photovoltage are different from that of other photoelectronic devices on the linked resistance.

The photo-voltage signals in bacteriorhodopsin(bR) excited by 1064 nm pulse laser are different from those by 532 or 355 nm. It shows that the positive and negative photoelectric signals are produced by the motion of the positive and negative charges, respectively, and more energy is needed for producing the positive charges than the negative. The mechanism of light-induced charge generation and charge transfer in bR was studied and analyzed by measuring the photoelectric signals with different impedance of measuring circuit and different pulse-width of 532 nm laser as pump light.