Objective:To explore the therapeutic efficacy of nebulized administration of Zhichuanling injection on allergic bronchial asthma in mice induced by ovalbumin（OVA）,and to investigate the safety of high-dose nebulization of Zhichuanling injection. Methods:Animal welfare and experimental procedures followed the regulations of the Ethics Committee of China Pharmaceutical University. The severity of asthma symptoms was assessed by quantification of the latency and frequency of asthma-like behavior in mice. H＆E staining was used to investigate histopathological changes in the lung. The total number of white blood cells（WBC）,eosinophil（Eos）,lymphocyte（Lym）and neutrophils（Neu）in bronchoalveolar lavage fluid were measured by automatic hematology analyzer to evaluate the allergic inflammatory response.Serum levels of interleukin-4（IL-4）and immunoglobulin E（IgE）were quantified using ELISA. The safety of high-dose nebulization of Zhichuanlin injection was evaluated by examining the general behavior and nervous system response of animals. Results:Our data demonstrated that the nebulized inhalation of different concentrations of Zhichuanling injection alleviated the asthma-like behavior of mice,improved the pathology of lung tissue,reduced the number of inflammatory cells in bronchoalveolar lavage fluid and the levels of IL-4 and IgG in the blood. Among the doses tested,the 1:1 dilution of Zhichuanling injection（the clinic dose）displayed the best efficacy that was comparable to the positive control,budesonide.Nebulized inhalation of a high dose（20×clinic dose）Zhichuanling injection did not affect the general behavior,nervous and autonomic nervous system,urine,feces,saliva,body weight and food intake of mice. Conclusion:Considering these data together,nebulized inhalation of Zhichuanling injection may represent an effective and safe drug administration route for bronchial asthma treatment.

目的 优选莲房黄酮苷最佳回流提取工艺条件. 方法 选取乙醇浓度(30％、50％、70％)、提取时间(60、90、120 min)、提取次数(1、2、3次)、料液比(1:12、1:14、1:16)作为考察因素,以杨梅素-3-O-β-D-葡萄糖醛酸苷、金丝桃苷、异槲皮苷、槲皮素-3-O-β-D-葡萄糖醛酸苷、山奈酚-3-O-β-D-半乳糖苷、异鼠李素-3-O-β-D-半乳糖苷、山奈酚-3-O-β-D-葡萄糖醛酸苷、丁香亭-3-O-β-D-葡萄糖苷8个黄酮苷总含量为考察指标,以HPLC法进行含量测定. 结果 最佳提取条件为16倍量70％乙醇回流提取3次,每次1h. 结论 正交试验优选出的莲房8个黄酮苷类成分总合量的提取工艺合理可行,为莲房黄酮类化合物的进一步研究提供参考.