探讨T复合体1(TCP1)在肝细胞癌(HCC)中的表达水平及临床意义.选取癌症基因组图谱(TCGA)数据库来源的HCC组织作为在线数据库研究对象,选取2018年5月—2019年5月新疆医科大学第一附属医院收治的53例HCC患者为临床研究对象,利用基因表达谱交互分析(GEPIA)数据库分析TCP1基因在HCC中的表达水平并用免疫组织化学方法进行鉴定,并分析TCP1的表达与HCC临床病理特征的关系及与患者预后的相关性.使用肿瘤免疫评估资源(TIMER)2.0数据库对不同表达的TCP1与免疫细胞浸润的相关性进行分析.TCGA数据库来源HCC肿瘤组织中TCP1的表达显著高于正常组织,临床来源的HCC组织中TCP1的免疫组织化学表达得分显著高于正常肝组织(6.32±1.24比0.54±0.18,t=21.533,P<0.001).免疫浸润分析结果显示TCP1与Th和Th2浸润正相关(r=0.390和0.384,P<0.001).GO/KEGG分析表明与TCP1共表达的基因在RNA定位、蛋白质定位等基因功能上发生富集,并且主要参与了细胞周期通路.预后生存分析结果显示,TCGA数据库来源和临床来源的HCC患者,TCP1低表达组的总生存率均明显高于TCP1高表达组(P<0.001).TCP1在HCC组织中表达上调,可以作为HCC有价值的预后标志物.

Liver cancer ranks fifth leading malignancy in incidence and third in mortality worldwide. Recently, its comprehensive treatment has greatly progressed; however, the prognosis is still poor due to difficulties in early diagnosis, high recurrence and metastasis rates, and lack of specific treatment. The search for new molecular biological factors that target the early diagnosis of cancer, predict recurrence, evaluate treatment efficacy, and identify high-risk individuals and specific therapeutic targets during follow-up becomes a great urgent task. circSOX4 is upregulated in lung cancer and plays the role of oncogene. This study attempted to assess circSOX4’s role in hepatocellular carcinoma (HCC). HCC tissues and cells were collected to measure circSOX4 level by qRT-PCR, cell behaviors by CCK-8 assay and Transwell assay, and relationship between circSOX4 and downstream targets by dual-luciferase gene assay and RIP. circSOX4 was upregulated in HCC tissue and cell lines, and its level was correlated with reduced patient survival. Interestingly, circSOX4 knockdown reduced HCC behaviors, glucose consumption, and lactate production. Furthermore, circSOX4 knockdown resulted in decreased in vivo tumor growth. circSOX4 was confirmed to target miR-218-5p, and the effect of circSOX4 downregulation on inhibiting tumor growth was diminished after miR-218-5p inhibition or YY1 overexpression in HCC cells. circSOX4 expression is closely associated with HCC through miR-218-5p and YY1-dependent pathways and may be a target and marker for HCC.

Background:Globally, liver cancer is one of the most common malignant tumors and is the third leading cause of cancer deaths. RNA-binding protein (RBP) is a general term for a class of proteins that bind to RNA to regulate metabolic processes. The expression of RNA-binding proteins is related to the prognosis of liver cancer patients.Methods:The RBP gene expression data of liver cancer were extracted from the TCGA database. First, the differentially expressed RBPs (DE RBPs) were selected through enrichment analysis and volcano mapping. Then, the prognosis-related RBP genes were selected through single-factor Cox regression analysis. The key prognosis-related RBPs were further screened by multifactor Cox regression analysis, and a formula for the patient's risk coefficient was obtained. Finally, based on the patient's risk score, a nomogram was established and verified.Results:We extracted 374 cancer tissue samples and 50 normal tissue samples with the clinical information from each sample. Through enrichment analysis, we screened 208 upregulated RBPs and 122 downregulated RBPs. Prognosis-related high-risk genes were EEF1E1, NOP56, UPF3B, SF3B4, SMG5, CD3EAP, BRCA1, BARD1, XPO5, CSTF2, EZH2, EXO1, RRP12, PRIM1, LIN28B, NROB1 and TCOF1, and the low-risk genes were MRPL46, RCL1, MRPL54, CPEB3, IFIT5, PPARGC1A, EIF2AK4, SEPSECS, ACO1, SECISBP2 L and ZCCHC24. Further multivariate Cox regression analysis was performed on the prognosis-related RBPs, and the three key prognosis-related RBPs were screened out, which were BARD1, NR0B1 and EIF2AK4. A patient risk coefficient calculation formula was obtained: risk score = (1.207×BARD1 Exp) + (0.483×NR0B1 Exp) + (-0.720×EIF2AK4 Exp). Finally, a nomogram was established based on the risk score to predict the survival time of patients from 1 to 5 years.Conclusions:The nomogram has good predictive value for the survival time of liver cancer patients.

目的 分析维汉两民族之间早期结直肠癌(colorectal cancer,CRC)患者肠道菌群的分布情况.方法 选取2019年3月至2020年3月于新疆医科大学第一附属医院首次手术并经病理学证实为早期CRC的患者83例.根据不同民族分为汉族组(43例)和维吾尔族组(40例),收集两组患者术前5 d内的新鲜粪便标本,进行粪便细菌培养和高通量测序,并对大肠埃希氏菌、具核梭杆菌、梭状芽胞杆菌、脆弱拟杆菌4种细菌进行实时定量荧光PCR反应检测,记录基因相对表达.结果 共鉴定出10个细菌门类和110个细菌属类.两组均以厚壁菌门、拟杆菌门、变形菌门、梭杆菌门的丰度较高.在属分类上而两组丰度较高的细菌属类有肠杆菌属、梭状芽胞杆菌属、拟杆菌属、梭杆菌属等.两组之间各细菌门以及细菌属水平上比较,差异均无统计学意义(P>0.05),且大肠埃希氏菌、具核梭杆菌、梭状芽胞杆菌、脆弱拟杆菌的基因相对表达比较,差异无统计学意义(P>0.05).结论 早期CRC患者中,拟杆菌属含量较高,其次为梭菌属、梭杆菌属、肠杆菌属,且在维汉两民族之间无差异.

目的:评估血清生长和分化因子15(GDF-15)与营养状态的相关性,为胃癌术后患者发生营养不良的临床诊疗提供新的临床思路和治疗靶点.方法:收集2019年3月—2020年3月收治的胃癌患者78例,根据TNM分期分为早期胃癌组和进展期胃癌组,分别在术前、术后14 d、术后30 d时收集两组患者的营养状态指标及GDF-15水平.用NRS-2002评分和PG-SGA评分评估患者的营养状态.结果:术后14 d时两组总白蛋白、白蛋白、前白蛋白均较术前降低(<0.05),而GDF-15、NRS-2002评分和PG-SGA评分均显著高于术前(<0.05).术后30 d时早期胃癌组的营养状态较术后14 d有所恢复,但进展期胃癌组总白蛋白、白蛋白、前白蛋白仍低于术后14 d(<0.05),GDF-15、NRS-2002评分和PG-SGA评分均显著高于术后14 d(<0.05).相关性分析结果显示术后14 d和术后30 d的GDF-15与前白蛋白显著负相关(=-0.872、-0.895,<0.05),与NRS-2002评分、PG-SGA评分正相关(=0.262、0.401和0.551、0.436,<0.05),在术后30 d时GDF-15与总蛋白质和白蛋白也表现出负相关性(=-0.598、-0.503,<0.05).结论:胃癌术后患者GDF-15水平与前白蛋白和PG-SGA评分密切相关,营养状态越差,GDF-15水平越高,因此GDF-15可以作为评估胃癌患者术后营养状态的潜在生物标志物.

目的 探讨术前外周血中性粒细胞与淋巴细胞比值(NLR)在胃癌根治术后患者预后中的评估作用.方法 选择2013年1月至2017年7月在新疆医科大学第一附属医院行R0胃癌根治术的166例胃癌患者为研究对象,全部患者术前行外周血细胞分类计数计算NLR,根据ROC曲线计算出NLR最佳临界点,将患者分为高NLR组(NLR≥1.74)117例,低NLR组(NLR<1.74)49例.比较两组患者临床病理特征和预后.结果 随访166例患者,其中低NLR组1年、3年生存率分别为91.84％、75.51％,高NLR组1年、3年生存率分别为90.60％、49.57％,单因素分析中维吾尔族、病理分型低分化组织类型、肿瘤N分期N1、N2、N3、TNM分期Ⅲ期、较高的NLR被确定为与总体生存期(OS)相关的不良预后因素;多因素分析中病理分型低分化组织类型、肿瘤T分期T3分期和NLR为与OS相关的独立预后因素.结论 术前NLR比值可作为R0胃癌根治术后患者生存率的独立预后因素,对评估患者预后有重要预测价值.

BACKGROUND:The dysregulated circular RNAs (circRNAs) are relevant to lung adenocarcinoma development. Nevertheless, the function and mechanism of hsa_circ_0020850 (circ_0020850) in lung adenocarcinoma development are uncertain.METHODS:A total of 35 lung adenocarcinoma patients were recruited, and the tumor and normal tissue samples were harvested. A549 and PC-9 cells were exhibited for the experiments in vitro. circ_0020850, microRNA-195-5p (miR-195-5p) and insulin receptor substrate 2 (IRS2) abundances were detected via quantitative reverse transcription-polymerase chain reaction or Western blot. Cell proliferation, apoptosis, migration and invasion were measured via cell counting kit-8 (CCK8) assay, colony formation, flow cytometry, transwell and Western blot. The relationship between miR-195-5p and circ_0020850 or IRS2 was tested via dual-luciferase reporter analysis. The function of circ_0020850 on cell growth in vivo was measured via xenograft model.RESULTS:circ_0020850 expression was enhanced in lung adenocarcinoma tissues and cells. circ_0020850 silence suppressed cell proliferation, migration and invasion and facilitated apoptosis. miR-195-5p was targeted via circ_0020850, and its knockdown reversed the inhibitive effect of circ_0020850 silence on lung adenocarcinoma development. IRS2 was targeted via miR-195-5p, and miR-195-5p inhibited cell proliferation, migration and invasion and induced apoptosis via decreasing IRS2. circ_0020850 knockdown decreased IRS2 expression via regulating miR-195-5p. circ_0020850 down-regulation decreased lung adenocarcinoma xenograft tumor growth.CONCLUSION:circ_0020850 knockdown repressed lung adenocarcinoma cell proliferation, migration and invasion and promoted apoptosis via regulating miR-195-5p and IRS2.