口腔白斑病(oral leukoplakia,OLK)作为一类常见的具有恶变潜能的口腔黏膜疾病,是发生在口腔黏膜上、不能擦去且不能以临床和组织病理学的方法诊断为其他疾病的一种白色损害,不包括局部刺激因素去除后可消退的单纯过角化症[1].OLK病因复杂,发病机制不明,可能与烟草、残冠残根等因素长期刺激,念珠菌感染和某些全身因素有关.

The three-dimensional (3D) printing technology combined with bone tissue engineering has become one of the major methods for mandibular reconstruction. However, the key factor retarding mandible reconstruction is the barrier of understanding and achieving the complex 3D gridwork formed by the trabeculae. This study innovatively constructed a low-temperature 3D printing silk fibroin/collagen/hydroxyapatite (SF/COL/HA) composite scaffold with a stable structure and remarkable biocompatibility. We designed three kinds of six-layer scaffolds with mixed fiber cross-angle structures (FCAS) of [0°/90°/0°/90°/0°/90°], [0°/45°/90°/135°/180°/225°] and [0°/30°/60°/90°/120°/150°]. Material properties of these scaffolds such as porosity, water absorption rate, X-ray diffraction, Fourier transform infrared spectroscopy, and compression performance were detected. Then, the MC3T3-E1 cells were seeded on these scaffolds and the adhesion, proliferation, and differentiation were investigated. To be more convincing, the same experiments were performed on another polycaprolactone/hydroxyapatite scaffold. The results suggested that the changes of FCAS affected the mechanical properties of 3D printed scaffolds and performance of seeded cells. Besides, the 90° FCAS significantly enhanced the compressive modulus in two groups and were more conducive to the cell proliferation and osteogenesis, which provided evidence for exploring the influence of FCAS on the properties of scaffolds and the application of two composite scaffolds in tissue regeneration.