Procedure for the synthesis of a cationic water-soluble Pd(II) complex with 9-diethylamino-5H-benzo[a]-phenoxazin-5-one (Nile red) [PdEnNR]OAc (where En is ethylenediamine, NR is deprotonated Nile red, OAc is outer-sphere acetate -ion) is developed. The positive solvatochromism of the luminescent properties of the Pd(II) complex has been established. The influence of DNA on the spectral-luminescent properties of [PdEnNR]OAc was studied. Intercalation of the complex into DNA in water was found.

To analyze the modification of the functionalized surface of graphene by protein molecules, a chemiluminescent enzyme immunoassay method was proposed. Using the example of functionalized graphene (FG) purification, the possibilities of chemiluminescent control of the state of its surface are shown. Methods for purifying FG from protein molecules with the restoration of the ability to resorb protein molecules are discussed. It has been shown that the FG surface can be cleaned from sorbed proteins, and a biosensor can be designed again based on such purified graphene, including with a different specificity. Therefore, the graphene sensor can be used repeatedly.