Procedure for the synthesis of a cationic water-soluble Pd(II) complex with 9-diethylamino-5H-benzo[a]-phenoxazin-5-one (Nile red) [PdEnNR]OAc (where En is ethylenediamine, NR is deprotonated Nile red, OAc is outer-sphere acetate -ion) is developed. The positive solvatochromism of the luminescent properties of the Pd(II) complex has been established. The influence of DNA on the spectral-luminescent properties of [PdEnNR]OAc was studied. Intercalation of the complex into DNA in water was found.