This paper proposes a technique to reform a lipid bilayer membrane by injecting an air bubble. Our group has been focusing on the method to form the artificial bilayer that can reconstitute membrane proteins. The bilayer can be formed at the interface of two water-in-oil droplets with a lipid bilayer. However, the bilayer often collapses and the droplets merges by either self-oscillation or sloshing. In the proposed technique, a bubble approximately 4 μL in volume is injected to split the droplet. When the bubble is retracted, the new bilayer is formed at the interface. The biggest advantage of this technique is that the re-formation of the artificial bilayer can be performed in situ and individually. The proposed technique was verified by measuring the current passing through the nanopore protein.