目的:应用S-100蛋白免疫组化染色,探讨其在早期麻风诊断中的作用.方法:将46例临床诊断为少菌型麻风,病理检查为非特异性炎症,未发现神经枝或神经枝结构正常的石蜡包埋块,重新作连续切片进行HE染色与S-100蛋白免疫组化染色,进行平行对比观察.结果:有11例(23.9%)显示神经内外炎性浸润.4例(8.7%)发现神经肉芽肿,两种方法具有显著性差异(P＜0.05).结论:S-100蛋白免疫组化染色在显示神经枝及其病变程度方面优于HE染色,有助于早期麻风的诊断,尤其是结核样麻风.

目的:探讨PCR、免疫组化应用于早期麻风诊断的价值.方法:采用PCR、免疫组化(PGL-1)和常规病理(HE、Fite抗酸染色)3种方法并用于临床诊断麻风病,用病理检查呈轻度非特异性炎症,查菌阴性的46例石蜡组织块,重新连续切片作对比观察.结果:3种检查结果比较,PCR阳性率(58.7%)＞PGL-1(36.9%)＞抗酸染色查菌AFB(15.2%),P＜0.05.结论:PCR、免疫组化两种检测技术,用于早期少菌型麻风的诊断比常规病理检查具有较高的敏感性和特异性.

Susceptibility to subcutaneous inoculation with M. lepraemurium, strain Hawaiian-B, of 4 mouse strains (CBA/J, CBA/N, DBA and C3H/Bi) was examined, concerning the relation between the clinical features of subcutaneous leproma at the inoculation site and the evolution of visceral lesions. Hawaiian-B strain has been maintained only by passages from C57BL/6 to C57BL/6 mice in our laboratory, since 1956. Mice of C3H, C3H/He, BALB/c, KK, DDD and C57BL/6 strains were also examined by the same manner above mentioned, as controls.Subcutaneous lesion developed a large and soft, typically malignant type, leproma at the inoculation site in CBA/J, CBA/N, DBA and C3H/Bi mice and extensive involvement was observed in their viscera, as observed in C3H mice. Taking the characteristics of above strains of mice into our consideration, CBA/J strain mice are much more suitable to use in mouse leprosy experiments as a representative of the typically malignant.