The aim of the study is the search of correlation dependence of infrared (IR) parameters of biological tissues of animals-carriers of tumours on concentration changes of some phosphorus-containing metabolites against the background of complex administration of ozone and doxorubicin (DR).Materials and methods. Experimental animals, white non-pedigree female rats (n=45), with tumour strain BC-1 (breast cancer) were administered intraperitoneal introduction of ozonized physiological saline (OPS) (ozone dose: 20 mg per an animal, 6 procedures every other day) interchanging it with DR administration (dosage: 0.04 mg per an animal, 5 procedures every other day). The animals were involved into the experiment on the 45th day after tumour subinoculation. The research was carried out using the technique of IR-spectroscopy of biological tissues.Conclusion. The administration of OPS, DR and OPS+DR complex to the animals with tumour strain BC-1 changes the parameters of IR spectra of the liver, kidneys, lungs, brain, tumour of these animals. OPS+DR complex has higher therapeutic effect compared to monochemotherapy. Therefore, ozone can be considered as a chemical agent modifying the cellular element action and enhancing tumour cells damage that emphasizes the feasibility of chemotherapy performed in combination with ozone therapy.

The aim of the study is the search of correlation dependence of infrared (IR) parameters of biological tissues of animals-carriers of tumours on concentration changes of some phosphorus-containing metabolites against the background of complex administration of ozone and doxorubicin (DR). Materials and methods. Experimental animals, white non-pedigree female rats (n=45), with tumour strain BC-1 (breast cancer) were administered intraperitoneal introduction of ozonized physiological saline (OPS) (ozone dose: 20 mg per an animal, 6 procedures every other day) interchanging it with DR administration (dosage: 0.04 mg per an animal, 5 procedures every other day). The animals were involved into the experiment on the 45th day after tumour subinoculation. The research was carried out using the technique of IR-spectroscopy of biological tissues. Conclusion. The administration of OPS, DR and OPS+DR complex to the animals with tumour strain BC-1 changes the parameters of IR spectra of the liver, kidneys, lungs, brain, tumour of these animals. OPS+DR complex has higher therapeutic effect compared to monochemotherapy. Therefore, ozone can be considered as a chemical agent modifying the cellular element action and enhancing tumour cells damage that emphasizes the feasibility of chemotherapy performed in combination with ozone therapy.

Aim of investigation is assessment of the ozonized physiologic salt solution low therapeutic concentration influence on a tumor medical pathomorphosis in experimental conditions.Materials and methods. An investigation is made on laboratory animals - white nonlinear female-rats (75 specimen) with a mass of 250 +/- 10g. An experimental model of neoplasia was created by twisting of the RMC-1 mammary gland cancer strain subcutaneously into the right axillary cavity area. The animals were included into experiment on the 45 days after a tumor twisting, a volume of which at that moment was 6-8 cm(3). A decapitation of animals and taking of a tumor tissue were made on the 11 days after the experiment beginning under an ether anesthesia. The ozonized physiologic salt solution was received by barbotage of a NaCl solution 0.9% with an ozonooxygenous mixture. A histological investigation of a tumoral tissue was accomplished by a generally accepted method. An analysis of induced biochemiluminescence and a measurement of the tumor tissue homogenate lipoperoxidation product levels were included into biochemical investigations.Results. A combined influence of the ozonized physiologic salt solution low therapeutic concentrations and a doxorubicin more expressively and destructively effected a tumoral tissue. A use of ozonized physiologic salt solution potentiated a doxorubicin antitumoral activity, which was manifested in a statistically more expressed depression of the tumoral cell mitotic activity and a decrease of their viable elements, causing the more profound manifestations of a therapeutic pathomorphosis.

Oxidation modification of proteins at a metabolic syndrome has been considered. An increase in spontaneous and induced oxidation modification of proteins in patients with this pathology has been observed. The changes in the level of spontaneous and induced oxidation modification of proteins at correction of metabolic disorders by low ozone doses and standard therapy have been studied.