目的:建立一种在大鼠体内示踪外部重组人胶原蛋白的方法.方法:采用超级生物素标记重组人胶原蛋白,将标记后的重组人胶原蛋白植入大鼠体内,实验周期结束后取植入部位组织,制备石蜡切片,用辣根过氧化物酶标记链霉亲和素识别病理切片上生物素标记的蛋白,二氨基联苯胺法染色(DAB法染色),显微镜观察组织情况.结果:在病理切片中观察到生物素标记重组人胶原蛋白的棕褐色沉淀,实现了植入组织的重组人胶原蛋白的定位.结论:生物素标记重组人胶原蛋白的方法灵敏度高,操作简单,可快速实现对重组人胶原蛋白的追踪.本文通过设立生物素对照组,排除生物素本身体内代谢对实验结果的影响.

As solitary filter feeder, Styela clava shows superior water purification capacity in the integrated multi-trophic aquaculture (IMTA) model consisting of S. clava, microalgae, and sea cucumber, which should be an ideal system to reduce organic and inorganic pollution in the water. It is of great significance to study the physiological energetics of S. clava for the application of this IMTA system. The physiological energetics of S. clava was determined in a simulated natural culture system to provide information for comparing to the energetic data derived from feeding ecological studies. The ascidians were grouped into five cohorts based on body size in terms of their wet weight of 1.5-2.0 g, 2.5-3.5 g, 5.0-6.5 g, 8.0-9.5 g, and 11.0-14.0 g, respectively. Clearance rate (CR), ingestion rate (IR), and assimilation efficiency (AE) of ascidian were estimated using flow-through feeding chambers. The results showed that the CR and IR increased exponentially with the ascidians’ body size at different temperatures. Body size showed little effect on AE at the same water temperature. However, significant differences were observed between different temperature regions in the range of 12-28°C regardless of body size. S. clava performed far better in feeding behavior at 20°C than that at higher or lower temperatures. Within the range of diet concentration (as particulate organic matter, POM) from 2.11 to 6.11 mg POM/L, the CR and IR increased exponentially with POM. This study provides basic data for the application of ascidian in the IMTA system.

Bioresorbable stents (BRS) are investigated and designed to revascularize occluded arteries. The iron-based bioresorbable stent is a promising device in interventional therapies, although it's corrosion and bioresorption rate remain challenging. In this work, we introduced a novel nitrided iron coronary stent (IBS) with enhanced degradation rate compared with pure iron stent. To evaluate the biosafety of this device, a sub-chronic systemic toxicity study was conducted and a stainless steel stent (Supporter™) served as a control. Here, the bioresorbable stent was first evaluated in rat abdominal aortic implantation model. When subjected to exaggerated exposure dose, no clinical signs of toxicity or mortality were observed in either, the IBS group or the control group. Histopathological examinations showed the corrosion particles of iron were encapsulated by fibrocytes and engulfed by macrophages, indicating that the degradation of iron was in the early stage. Our results demonstrated that the nitrided iron stent did not induce systemic toxicity under the experimental conditions.

目的:通过可降解防粘连材料的皮下植入实验,分析材料降解过程中组织细胞的反应,评价可降解丝素蛋白防粘连凝胶局部组织相容性,探讨材料体内降解过程,为研究可降解防粘连材料体内降解机制提供支持.方法:将实验样品(丝素蛋白防粘连凝胶)植入新西兰家兔脊柱右侧皮下,对照样品(聚乳酸防粘连凝胶)植入新西兰家兔脊柱左侧皮下,于植入后1周、4周、12周和26周两侧取样进行组织病理学观察及降解情况研究,并对材料周围细胞进行分类计数和评价.结果:材料周围组织病理学显示,12周时实验样品组纤维囊厚度小于对照样品组;细胞分析结果显示,实验样品组1周、4周主要以淋巴细胞浸润为主,炎症反应高峰在4周,对照样品组1周、4周样品周围以巨噬细胞为主,12周时出现大量淋巴细胞和浆细胞,反应高峰在12周,26周时两组炎症细胞均降为0;半定量评价结果显示,与对照样品相比,丝素蛋白防粘连凝胶植入局部无明显刺激.结论:根据植入材料的局部反应情况,推测丝素蛋白防粘连凝胶的降解过程主要是以淋巴细胞为主的炎性浸润;聚乳酸防粘连凝胶4周前以巨噬细胞介导的吞噬反应为主,同时伴有炎症浸润,12周时转变为以淋巴细胞为主的炎性浸润.