We have investigated in vitro the anticoagulant (AC) activity of proanthocyanidins from the bark of birch, cedar, spruce, pine, and larch; sulfated arabinogalantan and dihydroquercetin from larch wood; extracts from birch, cedar, and spruce; microcrystalline cellulose (MCC) from aspen and fir wood and wheat straw; and methylcellulose (MC) from aspen wood. The AC properties of the investigated substances are related mostly to their antithrombin activity. The AC activity increases with the content of sulfur in MCC of wheat straw, MC of aspen wood, and arabinogalañtan of larch wood. The maximum AC activity was observed in samples of sulfated MCC from fir wood and wheat straw. Their antithrombin activity (134 +/- 8 and 96 +/- 6, respectively) is worth of carrying out model tests in vivo.

Svetlana A. Kuznetsova: Institute of Chemistry and Chemical Technology SB RAS,Akademgorodok, Krasnoyarsk, 660036 Russia, Siberian Federal University, 79 Svobodny, Krasnoyarsk, 660041 Russia, Natalia Yu. Vasil’eva, Nadezhda M. Titova: Siberian Federal University,79 Svobodny, Krasnoyarsk, 660041 Russia; Galina S. Kalacheva: Institute of Biophysics SB RAS Akademgorodok, Krasnoyarsk, 660036 Russia; Evgenia S. Red’kina, Galina P. Skvortsova: Institute of Chemistry and Chemical Technology SB RAS,Akademgorodok, Krasnoyarsk, 660036 Russia

We have studied in vitro the ability of the Siberian cedar crust (SCC) extract (Pinus sibirica Du Tour) and arabinogalactan sulphate (AGS) extracted from wood of Siberiam pine-tree (Larix sibirica Ledeb.) to increase the human blood plasma coagulation time and also to inhibit the amydolytic activity of thrombin (aIIa) and the coagulation factor Xa (aXa). A method has been developed by means of which SCC increases the aXa activity by a factor of 3.7 and the aIIà activity by a factor of 2.5. The AGS preparation increased the blood plasma coagulation time in the test for activated partial thromboplastin time. An effective concentration, at which the time of plasma coagulation was increased by a factor of 2 (in comparison to the control) was 2.94 +/- 0.33 mg/ml. AGS did not exhibit the ability to inhibit the Xa activity.