The results of our clinical pilot study tkaneinzhenemoy design based on multipotent mesenchymal stromal cells of adipose tissue showed that the use of this construction leads to the restoration of bone tissue and stimulate reparative osteogenesis. And also to outline directions for further clinical study on the Application of LIC VT in maxillo-facial surgery.

Investigation of the mechanisms of regeneration of various organs and tissues using cellular technology has developed considerably over the last decade. Increased number of studies on the use of stem cells to correct defects in bone tissue dental surgery. Adipose tissue was used as a source of multipatent stem cells to produce tissue engineering. It was a clinical study on the restoration of bone tissue in patients with severe atrophy of the alveolar process of the maxilla with tissue engineering. To study the characteristics of the obtained bone regenerate within 4 months after transplantation examined histologically.

We performed a comparative study of reparative osteogenesis in rabbits with experimental critical defects of the parietal bones after implantation of commercial osteoinductive materials “Biomatrix”, “Osteomatrix”, “BioOss” in combination with platelet-rich plasma and transplantation of a tissue-engineering construct on the basis of autogenic multipotent stromal cells from the adipose tissue predifferentiated in osteogenic direction. It was found that experimental reparative osteogenesis is insufficiently stimulated by implantation materials and full-thickness trepanation holes were not completely closed. After transplantation of the studied tissue-engineering construct, the defect was filled with full-length bone regenerate (in the center of the regenerate and from the maternal bone) in contrast to control and reference groups, where the bone tissue was formed only on the side of the maternal bone. On day 120 after transplantation of the tissue-engineering construct, the percent of newly-formed bone tissue in the regenerate was 24% (the total percent of bone tissue in the regenerate was 39%), which attested to active incomplete regenerative process in contrast to control and reference groups. Thus, the study demonstrated effective regeneration of the critical defects of the parietal bones in rabbits 120 days after transplantation of the tissue-engineering construct in contrast to commercial osteoplastic materials for directed bone regeneration.

Use of synthetic osteoplastic materials not always leads to formation of the necessary bone tissue volume. The alternative high technological implantation material can become tissue engineering constructions containing osteogenic precursor cells. Clinical observation of sinus lifting with the use of tissue engineering construction demonstrates the efficacy, shortening the terms of operation wound healing, young bone tissue formation after transplantation and possibility to install implants already after 3 months if the amount of bone is sufficient for primary fixation.