Aim: to assess an effect of medicinal magnesium-containing composition on gelatinase B expression intensity and morphological parameters of chronic experimental endometrial inflammation.Materials and Methods. There were conducted experiments with 60 sexually mature female Wistar rats to determine an effect of medicinal magnesium-containing composition (contains magnesium chloride – a natural polymineral Bischofite) on gelatinase B activity in endometrial tissues (counting number of gelatinase-B-positive cells and intensity of gelatinase B expression), morphological parameters of cell infiltration, as well as amount of magnesium in red blood cell mass collected from the inferior vena cava and subclavian vein. All such parameters were assessed in experimental animals from 4 groups: group 1 – animals in baseline state, group 2 – control, group 3 – experimental chronic endometritis (CE), group 4 – experimental CE after treatment with medicinal magnesium-containing composition. Results. It was found that use of medicinal magnesium-containing composition increased amount of erythrocyte magnesium up to the baseline level and increased both number and expression intensity of stromal gelatinase B-positive cells. In addition, magnesium level in erythrocyte mass from the inferior vena cava was increased and accompanied with restored eosinophil-plasmacyte as well as significantly elevated macrophage-lymphocyte infiltration in endometrial tissues compared to CE animals lacking therapy. Conclusion. The results of this study allow us to conclude about importance of gelatinase B in pathogenesis of experimental chronic endometrial inflammation as well as an opportunity of regulating gelatinase B activity by applying medicinal magnesium containing drug in pathogenetic therapy of experimental CE.

Objective: to determine the specifi c number and expression intensity of gelatinase B-positive cells in the gastric surface epithelium and lamina propria (cell component) in the area of the ulcerous defect in rats with experimental acetate ulcer of the stomach.Materials and methods: 18 white Wistar rats were divided into three groups: initial (intact), experimental (modeling of an acetate ulcer) and control (falsely operated) in the experiment. Tissues were obtained from the ulceration zone or the presumed ulceration zone (in the initial and control groups) to asses morphological changes and also indicators, characterizing the activity of gelatinase B 7 days after the simulation of the experiment.Results and Discussion. It was revealed some increase in the specifi c number of gelatinase B-positive cells against the background of moderate accumulation of lymphocytes in the integument epithelium of the gastric mucosa. A signifi cant increase in the specifi c number of gelatinase B-positive cells at the maximum intensity of its expression and a moderate accumulation of eff ector cells of tissue alterations was determined in the cellular component of the lamina propria of the gastric mucosa at the same time.

The article highlights the classification and biological role of matrix metalloproteinases in such pathological processes as morphogenesis, angiogenesis and embryogenesis. The influence of metalloproteinases in mechanisms of formation and development of non-differentiated connective tissue dysplasia, endometriosis, wound process and tumor growth is shown.

By means of immune-histo-chemical method it has been revealed that at formed acetate ulcer in the fabrics of ulcerous defect in apical and cell component of mucous plate stroma the number of gelatinase B-positive cells number and its intensity increase. At the same time magnesium concentration in red blood cells, withdrawn from subclavian vein, decreases. Simultaneously negative correlation dependence between magnesium level and gelatinase B expression.

In rats with generated acid-induced gastric ulcer the magnesium level is significantly decreased in red blood cells from subclavian vein; the process of lipid peroxidation was rather active against the background of the antioxidant activity reduction. The area of the ulcerative defect decreased under the influence of the Mg2+-containing composition. Magnesium content increased in red blood cells from the subclavian vein and still more from the portal vein, whereas its plasma level was practically invariable. Malon dialdehyde content in blood from the portal vein and diene conjugate content in blood from the subclavian vein reduced at simultaneous increase of catalase and superoxide dismutase activity in the mass of red blood cells obtained from both collectors.