The report presents the results of the study of the chemical composition of of biologically active substances of various parts and organs of Agastache rugosa introduced in Western Siberia. Agastache rugosa were collected by the authors in July-August 2021–2022 in the territories of Tomsk regions. Water and water-alcohol extracts were obtained from raw materials. The studies was conducted using PC, TLC, UV-spectroscopy, titrimetry. The component composition of the essential oil was studied by GLC-MS on the gas chromatograph Agilent 6890 with quadrupole mass spectrometer (HP MSD 5973N). About 50 individual components were found, 30 of them for the first time. Vitamins (ascorbic acid, carotenoids), polysaccharides, phenolic compounds (flavonoids, phenolcarbonic acids, tannins), essential oil were found in all organs of the plant. Saponins are present in flowers and herbs, but not found in fruits. The presence of acacetin, apigenin, quercetin, tilianine, rosemary acid, caffeic acid, chlorogenic acid was found. The difference in the content of biologically active substances in different organs was established. The dominant groups in different parts and organs of the plant are essential oil, phenol carboxylic acids, carotenoids, ascorbic acid. Flavonoids and chlorophyll in all organs of this species belong to the group of minor BAS.

The search for new drugs to reduce the toxic effects of alcohol on the body is based on the creation of various pharmaceutical agents that affect the toxicokinetics and/or toxicodynamics of ethanol. A differentiated approach is needed in the pharmacotherapy of alcoholism due to the different clinical typologies of the condition. Compared with synthetic drugs, herbal remedies have multiple types of activity, which complement their efficacy. Research and development of technology for the manufacture of new drugs based on the hairy agrimony ( Agrimonia pilosa ) are promising. Experimental studies in which white outbred mice were tested in an open field showed that a hairy agrimony extract reduced orientational-exploratory behavioral activity and contributed to adaptation of the animals to stress. Mice treated with agrimony extract showed a reduction in sensitivity to administration of ethanol: the latent period of falling asleep was reduced by a factor of 1.6 and the duration of sleep decreased by a factor of 3 compared with the group of mice given starch paste. Rats treated with agrimony extract at doses of 100 and 150 mg/kg showed reductions in the duration of ethanol-induced sleep by factors of 1.8 – 2.4 as compared with animals treated with St. John’s wort extract, indicating that the extract had a detoxifying effect. Hairy agrimony extract significantly reduced grooming in all groups of animals, this being associated with its anti-anxiety effect and of great importance in terms of reducing the development of delirium in acute alcohol intoxication.

Поиск новых средств для снижения токсических эффектов алкоголя на организм основан на создании различных фармацевтических средств, влияющих на токсикокинетику и/или токсикодинамику этанола. Необходим дифференцированный подход при фармакотерапии алкоголизма из-за различной клинической типологии заболевания. В сравнении с синтетическими препаратами, растительные средства обладают несколькими видами активности, дополняющими эффективность их действия. Исследование и разработка технологии изготовления новых лекарственных средств на основе репешка волосистого (Agrimonia pilosa) являются перспективными. В ходе проведения эксперимента при тестировании белых беспородных мышей в «открытом поле» установлено, что экстракт репешка волосистого уменьшал ориентировочно-исследовательскую активность поведения и способствовал адаптации животных к стрессу. У мышей, получавших экстракт репешка волосистого, снижалась чувствительность к введению этанола: латентный период засыпания сократился в 1,6 раза, а длительность сна — в 3 раза по сравнению с группой мышей, получавшей крахмальную слизь. У крыс, получавших экстракт репешка волосистого в дозах 100 и 150 мг/кг, длительность этанолового сна уменьшалась в 1,8 – 2,4 раза по сравнению с животными, получавшими экстракт зверобоя, что свидетельствует о детоксикационном эффекте данного экстракта. Экстракт репешка волосистого значительно снижал груминг во всех группах животных, что связано с его противотревожным действием и является крайне важным для редукции развития делирия при острой алкогольной интоксикации.

Introduction. Lemna minor L. (duckweed) refers to the duckweed subfamily (Lemnaceae S. F. Gray) and widely distributed in ponds of Russia. Literature data confirm the possibility of harvesting significant volumes of this raw material in natural habitat and grown in aquaculture. The process of biosynthetic accumulation in duckweed fronds provides a variety of compounds with a wide spectrum of biological activity. Therefore, the use of raw materials Lemna minor L. is promising for the development of drugs and parapharmaceutical products. Thus, it is an urgent task to quantify active components of duckweed and standardize (determination of criteria for identification, quality and safety) plant material.Aim. Establish macro- and microscopic characteristics of raw materials and develop methods for the quantitative determination of the main groups of biologically active substances (BAS) for standardization of raw duckweed.Materials and methods. Samples of duckweed was collected in natural habitats of Western Siberia. Macro- and microscopic assay, HPLC, UV-spectrometry were used in research process.Results and discussion. Were established the criteria for identification of duckweed fronds by studying external (macroscopic) and microscopic features of raw material Lemna minor L. Was developed and validated the procedure of the quantitative determination of phenolcarboxylic acids in raw material Lemna minor L.Conclusion. The study of external (macroscopic) and microscopic features provided the criteria for identification of the raw material Lemna minor L. The technique for the quantitative analysis of polysaccharides using gravimetry does not need validation, because is a direct method of substance measurement. Was validated quantification method of phenolcarboxylic acids (in terms of chlorogenic acid) by criteria of linearity, repeatability, in-laboratory precision and accuracy. Was established quality criteria for identification and quantitative assay, which can be used in the draft for normative documents for medicinal plant raw material of Lemna minor L. «Duckweed fronds».

This study is aimed at investigating effects of pre-sowing seed treatment of Medicago sativa L. nothosubsp. varia (Martyn) Arcang., Sarga variety, with SiO2 nanoparticles produced by laser ablation in a liquid. The purpose of the study is to investigate effects of suspensions of silicon dioxide nanoparticles at different concentrations on biological and physiological characteristics of M. sativa L. nothosubsp. varia in natural agroclimatic conditions of the subtaiga zone in Western Siberia. The study has shown that the pre-sowing seed treatment of M. sativa L. nothosubsp. varia (Martyn) Arcang., Sarga variety, with silicon dioxide nanoparticles at different concentrations has a stimulating effect on contents of chlorophyll, carotenoids, and non-steroidal phytoestrogens in a vegetative part of the plant, as well as biometric and morphometric parameters, yield, and phytoremediation potential. It has been established that M. sativa is hypersensitive to the studied pre-sowing seed treatment with suspensions of SiO2 nanoparticles at different concentrations, which determines the specificity of changes in physiological parameters, including increase in contents of photosynthetic pigments, isoflavonoids, and hydroxycinnamic acids in the plant exposed to suspensions of SiO2 nanoparticles. A significant increase in the content of biologically active substances under the influence of SiO2 nanoparticles indicates the high sensitivity of M. sativa and the specific role of nanoparticles in stimulating the biosynthesis of biologically active substances. The findings of the study demonstrate that pre-sowing seed treatment of M. sativa L. nothosubsp. varia (Martyn) Arcang., Sarga variety, with ultrafine silicon dioxide nanoparticles at different concentrations, had a stimulating effect on biological and physiological characteristics of M. sativa L. nothosubsp. varia (Martyn) Arcang., Sarga variety.

Одной из причин развития метаболических и дегенеративных заболеваний костей и суставов является дефицит / нарушение обмена элементов. Существующие препараты не могут в полной решить указанные проблемы и имеют ряд противопоказаний и побочных эффектов. На этом фоне особую роль в профилактике и комплексном лечении заболеваний ОДС приобретают источники макро- и микроэлементов природного происхождения. В статье приведены результаты исследования содержания кремния, бора, кальция, магния в некоторых дикорастущих и интродуцированных растений методом атомно-эмиссионного анализа с индуктивно-связанной плазмой. Содержание элементов в растениях сравнивали с нормами «адекватного уровня потребления», принятыми в России. Результаты показали, что к числу перспективных видов относятся хвощи, береза, сосна, медуница, хмель, тысячелистник, спорыш, виды крапивы, кориандр, базилик, мята, мелисса, абрикосы, гречиха, малина, хмель, полынь.

Проведено исследование элементного состава климактерического сбора, рекомендуемого для профилактики и лечения патологических проявлений климактерического синдрома. Методом атомно-эмиссионного анализа с индуктивно-связанной плазмой в сборе идентифицировано более 30 химических элементов. Содержание элементов в сборе сравнивали с нормами «адекватного уровня потребления», разработанными для 20 элементов и введенными в России в 2005 году. Результаты показали, что сбор является ценным источником хрома, серебра, кремния, бора, кальция, магния и может явиться дополнительным фактором, вносящим ценный вклад в комплексное фармакологическое действие, наряду с другими группами биологически активных веществ. Содержание тяжелых металлов, таких как свинец, мышьяк, кадмий, в сборе не превышает предельно допустимые концентрации и соответствует требованиям Сан ПиН 2.3.2.1078-01.

A study of the elemental composition of 10 species of flora horsetail Siberia using neutron activation and X-ray fluorescence analysis methods. Established the presence of 38 chemical elements. In the systematically closely related species within a single subgenus, installed a similar elemental composition. Identified items that as phenolic compounds, can be considered an additional chemotaxonomic marker for the genus, subgenera and species. A horsetail species which contain a lot of silicon, manganese, iron, copper and zinc were identified. These species can then be used to create on the basis of their biologically active complexes preparations for the correction of mineral balance. The content of heavy metals such as lead, arsenic, cadmium assembly does not exceed the maximum allowable concentrations and meets the sanitary requirements 2.3.2.1078-01.

This article presents the results of a comparative study of the Si content in the plants used in medicine. Research method was a spectrophotometric assay based on the ability of silica to produce molybdenum ions in the acidic environment silicomolybdic soluble acid. The study, allowed to offer all the studied plants Siberian region, as a source of Si in herbal medicine pulmonary tuberculosis.

The results of research of phenolic compounds in an elevated part of a horsetail wood, growing in Tomsk and Kirov Regions are presented. The quantitative estimation of flavonoids and phenolic acidsspent of spectrophotometric method. Qualitative structure of phenolic compoundsstudied by chromatography. It is established that the Siberian samples have more various structure flavonoids and phenolic acids. Nine constant substances for samples of a horsetail wood in independence of a growth place are defined.

Quality of medicinal vegetative raw material depends on the contents of not only biologically active ma-terials, but also of chemical elements. The contents of microcells-biophiles (Mn, Zn, Cu), heavy metals (Pb, Cd, Sb, Hg, Cr, Ni) in wild-growing medicinal plants in Tomsk and Kemerovo oblasts are re-searched. It is established, that concentration of some heavy metals in medicinal plants exceeds tolerance levels. The element chemical compound of plants of the researched territory can be considered as reflec-tance of a biogeochemical situation in regions with disturbed natural biogeochemical cycles of elements.