The study of the phenolic compounds in aboveground organs of Euphrasia pectinata Ten. growing in the Baikal region. This research was done with the HPLC method and was studied with «GILSTON» instrument. 15 compounds were identified (flavonoids, phenolic acids, coumarins, tanninum). Predominant components: dihydroquercetin, luteolin, diosmetin-7β-D -glucuronozide; phenolcarbonic acids gallic acid, caffeic acid.

A unified procedure has been developed for the spectrophotometric determination of zovirax (aciclovir) in the substance using potassium ferrocyanide as reference sample. The best conditions for substance determination have been substantiated as follows: 0,1M NaOH as a solvent and an analytical wavelength of 261 nm. The coefficients of recalculation have been determined. The relative standard deviation of the developed methodology is 0,005 for the substance.

A unified procedure was developed for the spectrophotometric determination of drotaverine in the medicinally substance and using potassium dichromate as reference sample. The best conditions for substance the determination have been found as follows: 0,1M HCl as a solvent and an analytical wavelength of 353 nm. The scaling factors have been determined. The relative standard deviations of the results obtained with the proposed are less than 0,005 for the substance.

With the help of mathematical planning an experiment by the method of Latin square there was studied the infl uence of two factors on chromatographic behavior of pikamylone, nicotine acid and acids ƒ-aminobutyric. As factor A there were used the diff erent organic solvents, factor B icy acetic acid and 25% solution of ammonia, which infl uence upon pH systems of solvents. It has been determined that the second factor infl uenced upon the chromatographic behaviors of pikamylone, nicotine acids and acids ƒ-aminobutyric. Th e conditions of chromatographic analysis have been optimized and the method of control of pikamylone purity by the method of thin-layer chromatography has been developed.

Optimum conditions for carrying out the test dissolution of bendazol in tablets: the medium of dissolution 0,1 M solution of acid hydrochloride, volume of the medium of dissolution 1000 ml; speed of rotation 100 rev/min; time of dissolution 15 minutes. The unified technique of spectrofotometric definition of basket bendazol in the medium of dissolution, differing by use as the standard sample of phenolphthalein and acid benzoic has been developed. Optimum conditions of definition are proved: solvent 0,1M solution of acid hydrochloride. Results of definition of bendazol in the medium of dissolution have shown, that for 30 minutes of dissolution not less than 90% of bendazol from content of the medicinal form is released.

Th ere have designed unifi ed methods of spectrofotometric determination of pirazinamid in substances and tablets, diff ering with use as standard sample of the acid benzoic and potassium chromate. Optimum conditions of the determination, solvents water cleaned, sodium hydroxide (for potassium chromate), acid hydrochloride (for acid benzoic). Were proved the analytical wavelength 268 nm. Th e scaling factors have been determined. Calibration equation was given. Th e relative standard defl ection of the designed methods for substance has formed less than 0,005, for tablets 0,024.

A new version of the spcctrophotomctrical drugs analysis has been developed and approved. The analysis is characterised by the usage of an external comparison specimen. We suggest using the substances of HCI-typc purity as the external comparison specimen, with the quantity and qualrty of potassium chromate, potassium dichromatc, potassium ferricyanidc, phcnolphthalein, benzoic acid, adenine and guanine specified by the relevant National Standards ((iOS'l'). Several widely used medicines were exposed to the spectrophotomctrical analysis of this kind and it was demonstrated that the results obtained by this method met the standards set by the appropriate specifications with the error not exceeding 1,3 %. The suggested method is characterised by its accessibility, speed, simplicity and noninvolvcnient of toxic reagents.