Near‐isogenic lines (NILs) were developed in barley (Hordeum vulgare L.) and evaluated for resistance to barley stripe rust (BSR; incited by Puccinia striiformis f. sp. hordei) and agronomic potential. These NILs, the BISON lines (Barley stripe rust resistance ISOgeNic), represent BSR resistance quantitative trait loci (QTL) alleles introgressed individually and in all possible combinations into a susceptible background ‘Baronesse’ (PI 568246). The lines are BISON 1H (Reg. No. GS‐4, PI 659445), BISON 4H (Reg. No. GS‐8, PI 659449), BISON 5H (Reg. No. GS‐10, PI 659451), BISON 1H +4H (Reg. No. GS‐5, PI 659446), BISON 1H+5H (Reg. No. GS‐7, PI 659448), BISON 4H+5H (Reg. No. GS‐9, PI 659450), BISON 1H+4H+5H (Reg. No. GS‐6, PI 659447), BISON 7H (Reg. No. GS‐11, PI 659452), and BISON 0‐QTL (Reg. No. GS‐12, PI 659453), and the QTL donors were BCD12 (Reg. No. GS‐2, PI 659443), BCD47 (Reg. No. GS‐3, PI 659444), and D3–6/B23 (Reg. No. GS‐1, PI 659442). The experimental lines and released line names are the same. Phenotypic data, in conjunction with genotypic data, were used to characterize QTL allele introgressions and to assess the impact of these introgressions and pyramiding on agronomic performance. The BISON lines represent valuable sources of BSR resistance in an improved background, and performance data provide useful assessments of QTL allele introgressions (alone and in combination) and quantitative versus qualitative disease resistance.

Malting quality data were collected on malts from three barley (Hordeum vulgare L.) breeding program trials. We tried to identify causal polymorphisms in the Bmy1 intron III and coding regions for use in marker‐assisted selection. Abundant malting quality variation exists in the spring barley germplasm despite the parents having identical Bmy1 intron III and coding regions. After complete Bmy1 sequencing, no polymorphisms associated with malting quality phenotypes, indicating the genetic basis for the observed variation resides outside Bmy1. Complete allele sequencing identified one winter barley parent that had a novel Bmy1 allele (Sd1a) based on amino acid substitutions that are candidates as causative agents for the phenotypic variation. Marker‐assisted selection against the Sd1a allele could be effective in improving diastatic power (DP). The Sd1a allele is associated with low DP and is present in only three of the 51 lines, presumably due to preceding generations being selected for high DP. Selection for DP has subsequently eliminated the Sd1a allele from this breeding program. This research shows the importance of having complete allele sequences and knowledge of functional polymorphisms in target genes before using marker‐assisted selection.

The limited population sizes used in many quantitative trait locus (QTL) detection experiments can lead to underestimation of QTL number, overestimation of QTL effects, and failure to quantify QTL interactions. We used the barley/barley stripe rust pathosystem to evaluate the effect of population size on the estimation of QTL parameters. We generated a large (n = 409) population of doubled haploid lines derived from the cross of two inbred lines, BCD47 and Baronesse. This population was evaluated for barley stripe rust severity in the Toluca Valley, Mexico, and in Washington State, USA, under field conditions. BCD47 was the principal donor of resistance QTL alleles, but the susceptible parent also contributed some resistance alleles. The major QTL, located on the long arm of chromosome 4H, close to the Mlo gene, accounted for up to 34% of the phenotypic variance. Subpopulations of different sizes were generated using three methods-resampling, selective genotyping, and selective phenotyping-to evaluate the effect of population size on the estimation of QTL parameters. In all cases, the number of QTL detected increased with population size. QTL with large effects were detected even in small populations, but QTL with small effects were detected only by increasing population size. Selective genotyping and/or selective phenotyping approaches could be effective strategies for reducing the costs associated with conducting QTL analysis in large populations. The method of choice will depend on the relative costs of genotyping versus phenotyping.

The identification and location of sources of genetic resistance to plant diseases are important contributions to the development of resistant varieties. The combination of different sources and types of resistance in the same genotype should assist in the development of durably resistant varieties. Using a doubled haploid (DH), mapping population of barley, we mapped a qualitative resistance gene ( Rpsx) to barley stripe rust in the accession CI10587 (PI 243183) to the long arm of chromosome 1(7H). We combined the Rpsx gene, through a series of crosses, with three mapped and validated barley stripe rust resistance QTL alleles located on chromosomes 4(4H) (QTL4), 5(1H) (QTL5), and 7(5H) (QTL7). Three different barley DH populations were developed from these crosses, two combining Rpsx with QTL4 and QTL7, and the third combining Rpsx with QTL5. Disease severity testing in four environments and QTL mapping analyses confirmed the effects and locations of Rpsx, QTL4, and QTL5, thereby validating the original estimates of QTL location and effect. QTL alleles on chromosomes 4(4H) and 5(1H) were effective in decreasing disease severity in the absence of the resistance allele at Rpsx. Quantitative resistance effects were mainly additive, although magnitude interactions were detected. Our results indicate that combining qualitative and quantitative resistance in the same genotype is feasible. However, the durability of such resistance pyramids will require challenge from virulent isolates, which currently are not reported in North America.

Crop ScienceVolume 43, Issue 2 p. 729-731 Registrations of Cultivar Registration of ‘Tango’ Barley P.M. Hayes, Corresponding Author P.M. Hayes patrick.m.hayes@orst.edu Department of Crop and Soil Science, Oregon State University, Corvallis, OR, 97731 USACorresponding author (patrick.m.hayes@orst.edu)Search for more papers by this authorA.E. Corey, A.E. Corey Department of Crop and Soil Science, Oregon State University, Corvallis, OR, 97731 USASearch for more papers by this authorC. Mundt, C. Mundt Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, 97731 USASearch for more papers by this authorT. Toojinda, T. Toojinda DNA Fingerprinting Unit, National Center for Genetic Engineering and Biotechnology, Kasetsart University, Kampangsaen Campus, Nakorn Pathom, ThailandSearch for more papers by this authorH. Vivar, H. Vivar ICARDA/CIMMYT, Apdo. 6-641, Mexico 6, D.F., MexicoSearch for more papers by this author P.M. Hayes, Corresponding Author P.M. Hayes patrick.m.hayes@orst.edu Department of Crop and Soil Science, Oregon State University, Corvallis, OR, 97731 USACorresponding author (patrick.m.hayes@orst.edu)Search for more papers by this authorA.E. Corey, A.E. Corey Department of Crop and Soil Science, Oregon State University, Corvallis, OR, 97731 USASearch for more papers by this authorC. Mundt, C. Mundt Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, 97731 USASearch for more papers by this authorT. Toojinda, T. Toojinda DNA Fingerprinting Unit, National Center for Genetic Engineering and Biotechnology, Kasetsart University, Kampangsaen Campus, Nakorn Pathom, ThailandSearch for more papers by this authorH. Vivar, H. Vivar ICARDA/CIMMYT, Apdo. 6-641, Mexico 6, D.F., MexicoSearch for more papers by this author First published: 01 March 2003 https://doi.org/10.2135/cropsci2003.729aCitations: 10 Oregon Agricultural Exp. Stn. Manuscript No. 11893. Registration by CSSA. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume43, Issue2March–April 2003Pages 729-731 RelatedInformation

Crop ScienceVolume 35, Issue 5 cropsci1995.0011183X003500050046x p. 1504-1504 Registration of Cultivars Registration of ‘Maranna’ Barley P. M. Hayes, Corresponding Author P. M. Hayes n/a@.dne Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Corresponding author.Search for more papers by this authorA. E. Corey, A. E. Corey Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this authorR. Dovel, R. Dovel Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this authorM. Verhoeven, M. Verhoeven Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this authorW. E. Kronstad, W. E. Kronstad Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this author P. M. Hayes, Corresponding Author P. M. Hayes n/a@.dne Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Corresponding author.Search for more papers by this authorA. E. Corey, A. E. Corey Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this authorR. Dovel, R. Dovel Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this authorM. Verhoeven, M. Verhoeven Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this authorW. E. Kronstad, W. E. Kronstad Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this author First published: 01 September 1995 https://doi.org/10.2135/cropsci1995.0011183X003500050046xCitations: 1AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article.Citing Literature Volume35, Issue5September–October 1995Pages 1504-1504 RelatedInformation

Crop ScienceVolume 35, Issue 5 cropsci1995.0011183X003500050045x p. 1503-1503 Registration of Cultivars Registration of ‘Kold’ Barley P. M. Hayes, Corresponding Author P. M. Hayes n/[email protected] Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Corresponding author.Search for more papers by this authorA. E. Corey, A. E. Corey Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this authorM. Verhoeven, M. Verhoeven Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this authorM. Kolding, M. Kolding Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this authorW. E. Kronstad, W. E. Kronstad Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this author P. M. Hayes, Corresponding Author P. M. Hayes n/[email protected] Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Corresponding author.Search for more papers by this authorA. E. Corey, A. E. Corey Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this authorM. Verhoeven, M. Verhoeven Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this authorM. Kolding, M. Kolding Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this authorW. E. Kronstad, W. E. Kronstad Dep. of Crop and Soil Science, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this author First published: 01 September 1995 https://doi.org/10.2135/cropsci1995.0011183X003500050045xCitations: 1AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL No abstract is available for this article.Citing Literature Volume35, Issue5September–October 1995Pages 1503-1503 RelatedInformation