Previous studies indicated that the sense of body ownership (i.e., the feeling that our body parts belong to us; SBO) can be experimentally modulated in humans. Here, we focused on SBO from an across-species perspective, by investigating whether similar bottom-up and top-down constraints that consent to build SBO in humans also operate to build it in monkeys. To this aim, one monkey and a cohort of humans (N = 20) performed a paradigm combining the well-known rubber hand illusion (RHI), able to induce a fake hand embodiment, and a hand-identification reaching task, borrowed from the clinical evaluation of patients with SBO disorders. This task consisted of reaching one’s own hand with the other, while presenting a fake hand in different conditions controlling for bottom-up (synchronicity of the visuo-tactile stimulation) and top-down (congruency of the fake hand position relative to the monkey’s body) SBO constraints. Spatiotemporal kinematic features of such self-directed movements were measured. Our results show that, when the monkey aimed at the own hand, the trajectory of self-directed movements was attracted by the position of the hand believed to be one’s own (i.e., the fake hand), as in humans. Interestingly, such an effect was present only when both bottom-up and top-down constraints were met. Moreover, in the monkey, besides displacement of movement trajectory, also other kinematic parameters (velocity peak, deceleration phase) showed sensitivity to the embodiment effect. Overall, if replicated in a larger sample of monkeys, these results should support the view that human and non-human primates share similar body representation constraints and that they are able to modulate the motor behavior in both species.

Action and speech may take different forms, being expressed, for example, gently or rudely. These aspects of social communication, named vitality forms, have been little studied in neuroscience. In the present functional magnetic resonance imaging study, we investigated the role of insula in processing action and speech vitality forms. In speech runs, participants were asked to listen or imaging themselves to pronounce action verbs gently or rudely. In action runs, they were asked to observe or imaging themselves to perform actions gently or rudely. The results showed that, relative to controls, there was an activation of the dorso-central insula in both tasks of speech and action runs. The insula sector specific for action vitality form was located slightly more dorsally than that of speech with a large overlap of their activations. The psycho-physiological interaction analysis showed that the insular sector involved in action vitality forms processing is connected with the left hemisphere areas controlling arm actions, whereas the sector involved in speech vitality forms processing is linked with right hemisphere areas related to speech prosody. We conclude that the central part of the insula is a key region for vitality forms processing regardless of the modality by which they are conveyed or expressed.

In this fMRI study we evaluated whether the auditory processing of action verbs pronounced by a human or a robotic voice in the imperative mood differently modulates the activation of the mirror neuron system (MNs). The study produced three results. First, the activation pattern found during listening to action verbs was very similar in both the robot and human conditions. Second, the processing of action verbs compared to abstract verbs determined the activation of the fronto-parietal circuit classically involved during the action goal understanding. Third, and most importantly, listening to action verbs compared to abstract verbs produced activation of the anterior part of the supramarginal gyrus (aSMG) regardless of the condition (human and robot) and in the absence of any object name. The supramarginal gyrus is a region considered to underpin hand-object interaction and associated to the processing of affordances. These results suggest that listening to action verbs may trigger the recruitment of motor representations characterizing affordances and action execution, coherently with the predictive nature of motor simulation that not only allows us to re-enact motor knowledge to understand others' actions but also prepares us for the actions we might need to carry out.

The internal state of others can be understood observing their actions or listening to their voice. While the neural bases of action style (vitality forms) have been investigated, there is no information on how we recognize others' internal state by listening to their speech. Here, using fMRI technique, we investigated the neural correlates of auditory vitality forms while participants listened to action verbs in three different conditions: human voice pronouncing the verbs in a rude and gentle way, robot voice pronouncing the same verbs without vitality forms, and a scrambled version of the same verbs pronounced by human voice. In agreement with previous studies on vitality forms encoding, we found specific activation of the central part of insula during listening to human voice conveying specific vitality forms. In addition, when listening both to human and robot voices there was an activation of the posterior part of the left inferior frontal gyrus and of the parieto-premotor circuit typically described to be activated during observation and execution of arm actions. Finally, the superior temporal gyrus was activated bilaterally in all three conditions. We conclude that, the central part of insula is a key region for vitality forms processing allowing the understanding of the vitality forms regardless of the modality by which they are conveyed.