Abstract Rice Bran is the most basic and common raw material used in broiler feed, but its replacement with proceed palm kernel meal (palmofeed) adding with enzyme protease is not clearly evaluated. This study was conducted to evaluate the improvement of nutritional quality of proceed palm kernel meal with protease enzyme as a substitution for rice bran. Rice bran 7.85% and feed reformulation of proceed palm kernel meal with protease enzyme 7.85% on broiler performance and IOFC. A total 120 female broilers DOC CP 707 were randomly allocated into 2 treatments with 6 replicates and 10 birds per pen. Treatments were a control (T0) using standard diet nutrient requirement with 7.85% rice bran, treatment (T1) with feed reformulation of proceed palm kernel meal with protease enzyme 7.85%. The diets were fed in two phases as pre-starter (day 1-7) and grower to finisher (day 8-35). Compared to T0, the result showed that replacement rice bran 7.85% by feed reformulation of proceed palm kernel meal with protease enzyme 7.85% were not significantly (P<0.05) increase body weight (2194 and 2199) or weight gain (2151 and 2155), lower FCR (1.844 and 1.814) or mortality-corrected feed conversion ratio (mFCR) (1.326 and 1.375), performance index/PI (474 and 457) and IOFC (850, 491 and 922, 738). These data showed substitution of rice bran with processed palm kernel meal with enzyme protease does not has a negative impact on performance and is proven to be profitable from the point of view of IOFC business analysis.

Mastitis is a complex disease in cattle that it involves interactions between management practices and infectious agents. The common microorganisms causing mastitis are bacteria, besides this disease can be caused by mycoplasma, algae, and yeast. Pathogen microorganisms in milk can be obtained from cattle, human hands, equipment and the environment. This study aims to analyze the metagenomic of pathogen mastitis in cow’s milk from Cicurug, Sukabumi, West Java. ZymoBIOMICS TM DNA Miniprep Kit was used for genome isolation to metagenomic analysis. The 16S rRNA PCR amplification was used for analysis the results of miniprep. Metagenomic analysis from subclinical mastitis milk showed that bacteria in cow’s milk were the genera of Corynebacterium_1 (20.53 %), Corynebacterium (11.67%), Solibacillus (8.78%), Romboutsia (5.45%), Micrococcus (4.18%), Acinetobacter (3.64%), Aerosphaera (1.94%), Ignavigranum (1.90%), Lysinibacillus (1.49%), and Staphylococcus (1.38%).

The objective of this study was to evaluate the effect of noni (Morinda citrifolia) leaf shoot meal (NLSM) supplementation in the ration on the performances of kampong chickens as well as lipid, malondialdehyde (MDA), and fatty acid contents of meats. Two hundred and forty kampong chickens of 4 weeks old were reared until 12 weeks old. The diet treatments were R0: 0% NLSM (control), R1: 0.5% NLSM, R2: 1% NLSM, R3: 1.5% NLSM, R4: 2% NLSM, and R5: 2.5% NLSM. A completely randomized design (CRD) was used with six treatments and four replications with ten kampong chickens per replicate. The data were analyzed using ANOVA and if there were significant different, the data were further analyzed using Duncan’s Multiple Range Test. The variables measured were performances of kampong chickens as well as lipid, MDA, and fatty acid contents of meat. The result showed that cholesterol and LDL contents of meat were significantly reduced by feeding NLSM. Performance and HDL contents of meat were not significantly different for all diet treatments. In addition, feeding kampong chickens with ration supplemented with NLSM could reduce MDA, saturated fatty acids and increase unsaturated fatty acids contents of meat. It is concluded that 2.5% NLSM can be used to improve meat quality of kampong chicken as functional meat.

Background and Aim: Avian coronavirus has a wide range of hosts, from chickens and turkeys to wild birds. This virus causes an economically and, possibly, environmentally, important loss in the poultry industry. Therefore, research into the avian coronavirus in various species of birds is required. The Eclectus parrot (Eclectus roratus) is an endemic bird to Indonesia and Northern Australia and often kept as pets. At present, there has been limited information about avian coronavirus infection among birds. This study aimed to determine the presence of and to characterize avian coronavirus isolated from Eclectus parrots in Indonesia. Materials and Methods: Cloacal swab samples were taken from 10 healthy Eclectus parrots (E. roratus). Each isolate was propagated into specific pathogen-free embryonated chicken eggs. The presence of avian coronavirus was determined using three sets of primers targeting the 3' untranslated region (3'-UTR) of avian coronavirus (UTR41+/11–), the N gene of the infectious bronchitis virus (IBVN+/–), and the S1 gene of the IBV (XCE2+/XCE2–). The infectious bronchitis vaccine strain H120 was used as a positive control. Resulting positive bands were sequenced for the S1 gene. Results: None of the isolates was positive for the 3'-UTR, four isolates were positive for the N gene of infectious bronchitis, and two isolates were positive for the S1 gene of the IBV. However, only one isolate (parrot/Indonesia/BX9/16) was sequenced for the partial S1 gene with primers XCE2+/XCE2–. The partial nucleotide sequence of this isolate showed 100% homology with the IBV GI-13 lineage, specifically with a field isolate of the 4/91 variant 1 Israel and the 4/91 vaccine on the hypervariable region 3 site of the S1 gene. Conclusion: An IB-like avian coronavirus was isolated from healthy Eclectus parrots. Our results indicate that IBV has a wide range of hosts, which prompt the need to understand the interspecies connection of this virus better.

Sapindus rarak (lerak) is a native plant in Indonesia from the family of Sapindaceae.This plant has bioactive compounds known as saponins and is potentially to inhibit the growth of protozoa.The purpose of this study was to determine the histopathology changes in caecum of broilers that were infected with E. tenella through their litter, followed by an addition of S. rarak powder (SRP) with 2.5 g/kg in ration.A total of 90 birds consisted of 3 treatments with 3 replicates (10 birds per replicate) reared in litter system for 35 days.A completely randomized design was arranged for this experiment with R1 (Control, without coccidiostat, without SRP); R2 (Coccidiostat); R3 (SRP 2.5 g/kg).At 14 days old, all chickens were infected with E. tenella on the litter (15,000 oocysts/m).The parameters measured were macroscopic and microscopic changes of caecum.Chicken were dissected at day 7, 10, 13, 16, 19, 21 post-infection.The results showed that ptechiae of caecum appeared earlier on day 7 post infection in control (R1), while in SRP 2.5 g kg -1 (R3) and coccidiostat (R2) appeared on day 13 post-infection.Macroscopic and microscopic changes of caecum treated with SRP 2.5 g/kg treatment did not show severe lesions indicating that the E. tenella did not develop in caecum.It was concluded that saponins of S. rarak powder (SRP) at a dose of 2.5 g/kg inhibited the growth of E. tenella oocysts in the caecum of broiler chicken.

Curcumin, a curcuminoid compound of turmeric has been demonstrated to have anti-oxidant and anti-inflammatory properties. Bleomycin (BLM) is an anti-cancer drug induced pulmonary fibrosis in human and animals. This study was aimed to investigate biological effects of curcumin on bleomycin-induced pulmonary fibrosis in mice (Mus musculus) through pathomorphological assessment. In this study, 16 mice ddy strain were divided into four groups, namely (i) control, mice were subcutaneously (SC) injected with 100 µl sterilized aquadest in dorsal skin, (ii) BLM group, injected SC with 100 µl of 1 mg/ml BLM in dorsal skin, (iii) Curcumin (CMN) group, mice were intraperitoneally (IP) injected with 100 mg/kg body weight (BW) curcumin dissolved in 0,5% carboxy methyl cellulose (CMC) and injected with 100 µl sterilized aquadest SC, (iv) BLM+CMN group, injected SC with 100 µl of BLM 1 mg/ml and injected IP with 100 mg/kg BW CMN in 0,5% CMC. All treatments were performed daily for four weeks period. The lung samples were collected and fixed in buffered neutral formalin (BNF) 10%. Histopathological evaluation was performed with hematoxylin-eosin (HE) and Masson’s trichrome (MT) stains. The results showed that BLM treatment significantly increased fibrosis area and alveolar wall area fraction as compared to control. OIn the other hand, CMN treatment significantly reduced fibrosis area and alveolar wall area fraction in mice treated with BLM. In conclusion, our study showed that CMN treatment may inhibit lung fibrogenesis in BLM-induced pulmonary fibrosis.

Sapindus rarak is a species of soapberry and in Indonesia the trunks commonly used as board cast, match sticks and wooden crafts and the saponin containing fruits are used as a detergent for traditional clothes (called batik). Saponin has biological activity to damage protozoa. The study has been carried out to investigate the effects of particle size and type solvent on the concentration of saponin extracted from the Sapindus rarak pericarp powder (SRP) and to evaluate the activity of SRP on oocysts of Eimeria tenella in vitro. Completely randomized design with 2 factors was arranged to analyze saponin content. First factor was type of solvent, i.e., 70% methanol and water. Second factor was particle sizes of SRP, i.e., 75, 300 and 600 μm and the saponin analyses of each treatment was done in triplicates. Determination of total saponin as described by Hiai and Nakajima and total sapogenin according to Hiai method with some modification. In vitro assay was performed at several concentrations of SRP in water (100-0.01 mg/mL). Results showed SRP with 75 μm particle size showed the highest foam height and the highest total saponin content compared to those having 300 and 600 μm of particle size. Solvent and particle size significantly affected saponin content. Saponin content of SRP was higher when it was extracted with 70% methanol (35.98%) than extracted using water (32.53%). SRP with 75 μm particle size had higher saponin content (43.52%) than those with 300 and 600 μm particle size (37.54 and 21.71%). Sapogenin content extracted with 70% methanol and water was not significantly different (30.55 and 30.36%), but particle size affected the sapogenin content. At 75 μm particle size, the sapogenin content was significantly higher (35.51%) than 300 and 600 μm particle size (33.46 and 22.40%). The SRP with concentration of 100-1 mg/mL water inhibited sporulation of E. tenella oocysts more than 90%. It is proved SRP has the ability to destroy the development of Eimeria tenella.

Sapindus rarak fruits have bioactive compound named saponin which are potential to inhibit growth of protozoa and reduce cholesterol. This study was conducted to evaluate effect of lerak (Sapindus rarak) powder as microparticle on performance and lipid profile of chicken broiler that was infected by Eimeria tenella. A total of150headsof DOCwere assign to recive on of5 treatments: T1 (K+, TAk, TSRa, TIE), T2 (K-, TAk, TSRa, IE), T3 (Sal, IE), T4 (Sra 2,5, IE), and T5 (Sra 1,25, IE); each treatment consisted of 6replications with 5birds per replications in battery cage for 34 days. All chickens except those in control positive treatment (non-infected, non-medicated) were inoculated orally with 6000 oocysts E. tenella on the 14th day of age. Variables measured were body weight gain, carcass, OPG (oocysts pergram of faeces), lipid profile, and mortality. Treatment with S. rarak, dose of 1.25g/kg was not significantly different from the salinomycin treatment on BWG and feed conversion. Percentage of carcassand organs and blood cholesterol concentration were not significantly differentin all treatments, but blood triglyceride at S. rarak addition with doseof 2.5, 1.25g/kg and salinomycin addition with dose of 0.5g/kg, and negative control were lower than positive control. While number of OPG in faeces (days 14-34) showed that S. rarak powder with dose of 2.5 and 1.25g/kg suppressed the development of oocystsof E. tenella. In conclusion, S. rarak microparticle (75µm) can be used as feed additive to replace salinomycinas coccidiostat.

Propolis is a resinous substance collected by stingless bee or honey bee from various plant sources. The substance is known to contain beneficial properties for human. The geographical origin of propolis determines its biological properties. In this study, propolis were collected from five regions of Indonesia with the objective of determining the yield, their total flavonoid content, their capacity to induce apoptosis, and their toxicity to the Michigan Cancer Foundation-7 (MCF-7) cell line. The inhibition of antioxidant 1,1-diphenyl-2-picrylhydrazyl (DPPH), the induction of apoptosis to Saccharomyces cerevisiae and the anticytotoxic ability were determined. Propolis from Pekanbaru region had higher yield than other regions with value of 19.97%; propolis from Kendal had higher quantity with value of 46.60%, total flavonoid content; propolis from Pandeglang was higher in DPPH oxidation capacity with value of 68.94 mu g.ml(-1); propolis from Kendal, expressed petite cell induction in S. cerevisiae cells with value of 81.44%, and the anticytotoxic to MCF-7 breast cancer cell line were best observed in propolis from Makassar region with a value of 47.71% life cells. All of the propolis extracted from the stingless bee hive Trigona spp from five regions in Indonesia contained flavonoids.

Propolis adalah zat resin yang dikumpulkan oleh lebah madu (stingless bee dan honey bee) dari berbagai tanaman, memiliki berbagai aktivitas fisiologis seperti antioksidan, induksi apoptosis dan antikanker. Tujuan penelitian ini untuk menentukan kondisi terbaik ekstraksi propolis dan karakterisasinya sebagai bahan antikanker payudara. Ekstraksi dilakukan dengan teknik maserasi dengan pemanasan gelombang mikro dengan waktu pemanasan 10, 20 dan 30 menit. Tiga belas contoh propolis dari Pandeglang, Banten Indonesia digunakan untuk uji induksi apoptosis sel S accharomyces cerevisiae dan analisis data dilakukan menggunakan Response Surface Methodology (RSM). Waktu ekstraksi dan rasio pelarut etanol 70% sarang lebah terbaik yang terpilih dalam menginduksi apoptosis masing-masing selama 27 menit dan sebesar 20, dengan hasil ekstrak propolis sebanyak 12,67%. Selanjutnya, ekstrak propolis mempunyai konsentrasi dalam menghambat sebanyak 50% (IC 50 ), mematikan 50% sel lestari kanker Michigan Cancer Foundation -7 (MCF-7), dan induksi apoptosis S. cerevisiae masing-masing sebesar 75,34 µg. mL -1 , 233 µg.mL -1 , dan 6,015 µg.mL -1 . Hasil uji efikasi in-vivo terhadap sel kanker akibat induksi 7,12- dimethyl-benz(a)anthracene (DMBA) menunjukkan bahwa konsentrasi propolis 233 µg.mL -1 dapat menyembuhkan jaringan yang rusak akibat tumor. Kata kunci: propolis, induksi apoptosis, MCF-7, RSM, antikanker payudara

Jatropha curcas meal (JCM) contains high crude protein (58%-60%), but it can not be used properly because of anti-nutritional contents known as curcin and phorbolesther. These components interferes protein metabolism and body protein synthesis. This study was conducted to evaluate the effect of feeding fermented JCM using Rhizopus oligosporus on broilers’ performances. Ninety six of day old chicks of Ross strain broiler (initial body weight 45.6±1.7 g) were used and reared for five weeks. The treatments were: R0 (diet without JCM), R1 (diet contained 3% fermented JCM), R2 (diet contained 6% fermented JCM) and R3 (diet contained 9% fermented JCM). A completely randomize design with 4 treatments and 4 replications was assigned in this experiment. The data were analyzed using analysis of variance (ANOVA). The results indicated that feeding fermented JCM at the level of 3% to 9% in starter and grower-finisher broilers highly significant reduced (P<0.01) feed consumption, body weight and body weight gain. Feeding JCM at the level of 9% (R3) highly significant increased (P<0.01) the mortality rate of starter as well as grower-finisher period broilers. Fermentation of JCM using R. oligosporus indicated no effective detoxification process in relation to the improvement of broiler performances.

Jatropha curcas meal ( JCM) contain high protein, but its utilization as feed ingredient is limited by the presence of several anti nutritive and toxic compounds. A research has been conducted in the Faculty of Animal Husbandry IPB to determine the values of metabolizable energy, crude fiber digestibility, retention of nitrogen, calcium and phosphorous of JCM fermented using Rhizopus oligoporus or of fermented JCM suplemented with enzymes on chickens. In this experiment 25 of ten weeks old chickens were used (20 chickens were fed experimental diets, and 5 chickens were used to measure endogenous energy). A Completely Randomized Design (CRD) with 5 treatments and 4 replications was used in this experiment. The experimental diets were R0 = basal diet without JCM ; R1 = the diet contained unprocess JCM 5%; R2 = the diet contained fermented JCM 5%+ cellulase 20.000 U/kg; R3 = the diet contained 5% of fermented JCM + phytase 1000 FTU/kg; R4 = the diet contained 5% of fermented JCM + cellulase 20.000 U/kg + phytase 1000 FTU/kg. The parameters measured were Metabolizable Energy (ME, Men, TME, TMEn) digestibility of crude fiber and retention of calcium, phosphorus and nitrogen. The result indicated that fermented JCM suplemented enzym phytase, cellulase as well as enzym combination increased metabolizable energy. Nitrogen retention was improved highest significantly (P < 0.01) by feed of fermented JCM suplemented enzyme compared to control diet or diet containing untreated JCM. The fermented JCM suplemented with phytase enzyme (R3) gave the highest nitrogen retention (76.73%.). The highest value of digested crude fibre (24.65%) was on the diet containing fermented JCM suplemented with cellulase (R2). The calcium retention improved significantly (P < 0.05) with diet containing fermented JCM supplemented enzyme compared to that of the diet containing untreated JCM. It can be concluded that fermented JCM supplemented enzymes cellulase, phytase and its combination increased the value of metabolizable energy, digestibility of crude fiber and nitrogen retention. Key Words : Chicken, JCM Fermented, Enzymes, Metabolic Energy

The experiment was conducted to evaluate chemical characteristics and biological value of protein concentrate extracted from palm kernel meal (PKM) using combination of physical and chemical extraction. The best method of extraction in term of total amino acid (TAA), essential amino acid (EAA), essential amino acid index (EAAI), and protein recovery then was used for protein retention evaluation using 20 heads of male quails age 30 days. The animals were randomly assigned to one of three dietary treatments, while the remaining quails were used to measure endogenous protein. The treatments were; R1=diet containing 9% crude protein from the best protein concentrate from PKM, R2=diet containing 9% crude protein from PKM and R3=diet containing 9% crude protein from soybean meal). The results showed that protein of PKM had isoelectric pH in a range of 4.3–4.4. Protein concentrate of PKM produced by grinding using 0.05 N acetic acid followed by soaking 1 N technicalNaOH had the highest protein quality compared with those of the other treatments. TAA, EAA, EAAI amount of precipitate and protein recovery of the protein concentrate were 33.38%, 16.76%, 62.41%, 12.18% and 50.38%, respectively. Protein retention of quails fed R1 was not different compared to that of R3 (69,82 vs 70,57%), while quails received R2 had the lowest protein retention value (61,19%). It is concluded that combination methods of physical and chemical extraction using 0.05 N acetic acid followed by soaking with 1 N technical NaOH (E3) was the best method in producing high quality of protein concentrate, and the product had similar protein retention value as that of soybean meal.

The research was conducted using lemuru fish oil and vitamin E supplementation in broiler chicken diet as an immunomodulator. The experiment design was used a completely randomized design with 3 x 3 factorial patern. Nine treatment diets were consisted of three levels of lemuru fish oil supplementation (0, 3, and 6%) as the first factor, and vitamin E supplementation (0, 100, and 200ppm) as the second factor and its combinations. All data were analyzed by analysis of variance and Duncan multiple ranges. There were no significant differences among treatments on antibody titers responses after first ND vaccination, but gave significantly differences on antibody titers (P<0,05) after the second ND vaccination. There were interaction effects of dietary lemuru fish oil and vitamin E on the increasing of the antibody titers. There were no interaction effects of lemuru fish oil and vitamin E on antibody titers responses to IBD. Vitamin E supplementation significantly increased (P<0,05) antibody titers responses to IBD, but not the lemuru fish oil supplementation. The amount of lymphocyte was significantly increased (P<0,05) due to the lemuru fish oil supplementation but not due to the vitamin E supplementation. (Animal Production 10(2): 110-116 (2008) Key Words: Lemuru fish oil, vitamin E, broiler chicken, immunomodulator

Clinical Hlstory Animal . White Bengal Tiger Age 22 year Sex . Male S~nce 1 year ago, the tiger was noticed had a subcutaneous tumor mass at his left ear. The nodule was grwing bigger and bigger until the size of 10 x 15 x 5 cm. Recently, some small tumor masses were also noticed near his lefl eye, right ear and dorsal of the head. Signs of loss appetite and lethargy were observed and finally refused to eat for the last 1 week The tiger then was euthan~zed with pentobarb~tal

The experiment was conducted to evaluate chemical characteristics and biological value of protein concentrate extracted from palm kernel meal (PKM) using combination of physical and chemical extraction. The best method of extraction in term of total amino acid (TAA), essential amino acid (EAA), essential amino acid index (EAAI), and protein recovery then was used for protein retention evaluation using 20 heads of male quails age 30 days. The animals were randomly assigned to one of three dietary treatments, while the remaining quails were used to measure endogenous protein. The treatments were; R1=diet containing 9% crude protein from the best protein concentrate from PKM, R2=diet containing 9% crude protein from PKM and R3=diet containing 9% crude protein from soybean meal). The results showed that protein of PKM had isoelectric pH in a range of 4.3–4.4. Protein concentrate of PKM produced by grinding using 0.05 N acetic acid followed by soaking 1 N technical-NaOH had the highest protein quality compared with those of the other treatments. TAA, EAA, EAAI amount of precipitate and protein recovery of the protein concentrate were 33.38%, 16.76%, 62.41%, 12.18% and 50.38%, respectively. Protein retention of quails fed R1 was not different compared to that of R3 (69,82 vs 70,57%), while quails received R2 had the lowest protein retention value (61,19%). It is concluded that combination methods of physical and chemical extraction using 0.05 N acetic acid followed by soaking with 1 N technical NaOH (E3) was the best method in producing high quality of protein concentrate, and the product had similar protein retention value as that of soybean meal.

ABSTRACT A study was made to evaluate the cutoff value of indirect immunofluorescent-antibody (IFA) test for Q fever diagnosis in Japan. We used 346 sera, including 16 from confirmed Q fever cases, 304 from Japanese pneumonia patients, and 26 from negative cases. Thirteen sera from the confirmed Q fever cases with an immunoglobulin M (IgM) titer of ≥1:128 and/or IgG titer of ≥1:256 by the IFA test were positive by both enzyme-linked immunosorbent assay (ELISA) and Western blotting assay (WBA), whereas 298 sera from pneumonia patients and 26 negative sera with an IgM titer of ≤1:16 and an IgG titer of ≤1:32 by the IFA test were negative by both ELISA and WBA. In the proposed “equivocal area,” with an IgM titer of ≥1:32 and ≤1:64 and/or an IgG titer of ≥1:64 and ≤1:128, we found 9 sera, 3 from confirmed Q fever cases and 6 from Japanese pneumonia patients, by the IFA test. Three sera from the confirmed Q fever cases and one of the sera from pneumonia patients were IgM and/or IgG positive by both ELISA and WBA. These results suggest that a single cutoff value for the IFA test may cause false-positive and false-negative results. In conclusion, this study showed that an “equivocal area” should be used for the IFA test rather than a single cutoff value and that sera in the equivocal area should be tested by additional serological assays for confirmation.

Infectious bursal disease virus (IBDV) capsid protein, VP2, contains a hypervariable domain that is recognized by virus-neutralizing antibodies. The virus-neutralizing epitope is highly conformation-dependent and the domain is speculated to be involved in the virus-target cell interaction. In this study, a polyclonal anti-idiotypic antibody (anti-id) was generated by the sequential immunization of a rabbit with a virus-neutralizing monoclonal antibody GI-11 which recognizes the VP2 hypervariable domain. Although the anti-id, which mimics the conformational epitope in the VP2 hypervariable domain, was expected to inhibit the virus infection, the anti-id did not interfere with either the virus binding or the infection to the target cell.

Three hybridoma cell lines producing monoclonal antibodies (MAbs) against LSCC-BK3 cells which are susceptible to infectious bursal disease virus (IBDV) infection were produced and characterized. The MAbs, designated T7, Q11 and Q13, inhibited the attachment of IBDV to LSCC-BK3 cells. Furthermore, these MAbs bound to LSCC-BK3 but not to nonpermissive cells in flow cytometry. MAb T7 detected a 110-kDa membrane protein of LSCC-BK3 cells, whereas Q11 and Q13 reacted with membrane proteins of molecular weights 58-, 85-, 90- and 110-kDa. These observations imply that the 110-kDa protein recognized by all the MAbs is associated with IBDV binding. The MAbs established in this study are useful for studying the interaction between IBDV and its target cell.