The experiment was conducted for investigating the effect of Non-Starch Polysaccharide (NSP) feed containing wheat and barley on relative bioavailability values (RBVs) of minerals and supplementation in the broilers’ diet. A total of 72 male broiler chickens (Ross 308) were arranged with 4 repetitions (6 birds per each replicated in the metabolic cages) on the 3 treatment diets. The experimental diets contain one control diet and 2 diets with formulation of 25% wheat and 20% barley. Estimated relative mineral bioavailability values (RMBVs) were based on daily dietary mineral intake. The RBVs of Ca, P, Cu, Mn, Fe, and Zn were 44, 120, 17, 0.37, 125, and 15.38, respectively for dietary-containing wheat. Data showed that NSP contain a diet effect on bone characteristics and seed or index tibiae bone (P < 0.05). RMBVs of Ca, P, Mn, Fe, and Zn for a diet including wheat were 65.20, 84.96, 21.05, 8.50, and 2.56, respectively based on the tibia bone. Also, RMBVs of tibia bone minerals, such as Ca, P, Mn, Fe, and Zn from a diet containing barley, were 53.60, 43.33, 40.60, 44.16, and 44.69, respectively. The results of experiments indicated that Ca, P, and Fe have highly RMBVs concerning the amount of NSP contained in barley.

This study aimed to evaluate the relative bioavailability value (RBV) of different manganese mono oxide (MnO) sources and manganese sulfate (MnSO4) in broiler diets as well as their effect on tibial characteristics, and serum inorganic phosphorous (P), calcium (Ca) and Mn concentrations. The experiment was carried out based on a completely randomized design (CRD) with 660 broiler chicks (Ross 308) assigned to 12 dietary treatments with 6 replicates of 10 birds each. The experimental diets consisted of one basal diet (as control), and 10 treatment groups which were supplemented with 400 or 800 mg/kg of feed of 1-5 MnO sources with 35%, 25%, 45-55%, 30%, and 40% purities, respectively; MnSO4 was also used (800 mg/kg of feed) in a treatment group as a reference standard with 100% bioavailability. The mean RBV of MnO sources (27.27 to 181.82%) showed a significant difference (P<0.05). The results also showed sera Ca and Mn concentrations, and retention of Mn in diet and tibia bone were significantly affected by different Mn sources used (P<0.05). Although no differences were observed for sera P, Ca, and Mn concentrations, regardless of the level of supplementation (P<0.05). The results of this study demonstrated that the purity of Mn supplement sources has a relationship with RBV of Mn, Mn absorption, and its retention in broiler bone and ileum.

The objective of this study was to compare the expression of pituitary gonadotropin genes between three populations of native birds in Iran and Hyline W-36. One hundred and twenty-eight 37-week-old chickens (Golpayegan, Marandi, Isfahan, and Hyline W-36) were studied for 12 weeks. All birds were reared in the same cage system and under similar nutritional and managerial conditions. Quantitative characteristics such as egg number, egg weight, egg production percentage, egg mass, feed intake, and feed conversion ratio were calculated. At the end of the trial, five hens in each group were slaughtered after weighing, and their heads, livers, ovaries, and oviducts were removed. The gene expression of LH and FSH was determined by reverse transcription-polymerase chain reaction (RT-PCR), after RNA extraction from the pituitary gland. The results showed that Hyline W-36 had the highest egg number, egg weight, percentage of egg production, and egg mass. The feed conversion ratio was the highest in the Marandi, but the lowest was observed in the Hyline W-36. Expression of the FSH gene in Hyline W-36 was higher than in other groups. A positive and significant correlation was observed between the expression of the pituitary gland FSH gene, egg mass, and relative ovarian weight. The difference in production performance of various genetic populations seems to be mainly due to the differential expression of the FSH gene in the pituitary gland. Therefore, for genetic enhancement programs in native chicken, these results may help identify the metabolic pathways of gonadotropins gene expression, their effects on the ovaries, and reproductive activity.

This experiment was conducted to evaluate the effect of licorice (Glycyrrhiza glabra) essential oil (LEO) as an alternative to antibiotic growth promoter on growth performance, ileal microbial population, blood biochemical traits, and ascites sensitivity in broiler chickens. A total of 625 one-day-old broiler chickens were assigned to 5 dietary treatments, including control (un-supplemented basal diet), antibiotic (basal diet + 150 mg avilamycin/kg), probiotic (basal diet + 100 mg probiotic/kg), basal diet + 200 mg LEO/kg, and basal diet + 400 mg LEO/kg, in a completely randomized design, with 5 replicates of 25 broiler chickens per pen. Dietary inclusion of 200 mg LEO/kg increased feed intake compared to control and 400 mg LEO/kg treatments (P < 0.05). Broiler chickens fed the diet containing 200 mg LEO/kg showed greater weight gain and better feed conversion ratio (P < 0.05) compared to diets containing 400 mg LEO/kg. No differences were observed in feed intake and weight gain among diets containing 200 mg LEO/kg, antibiotic, probiotic, or control treatment. No differences in blood lipids, Alkaline phosphatase, and albumin were observed among all the treatments. The concentrations of total protein and globulin in serum of broiler chickens fed control diet were greater than broiler chickens that received antibiotic, probiotic, or 400 mg LEO/kg (P < 0.05). Diet containing 200 mg LEO/kg caused the greatest serum concentration of T3 or T4 (P < 0.05). The T4/T3 ratio in broiler chickens fed the diet containing 400 mg LEO/kg was greater than those fed the antibiotic or control diets (P < 0.05). Also, broiler chickens fed 400 mg LEO/kg diet had lower right ventricle /total ventricles ratio (RV/TV) than those fed the control, antibiotic, or 200 mg LEO/kg diets (P < 0.05). The ileal population of Escherichia coli in broiler chickens fed diets containing 200 or 400 mg LEO/kg was lower than those fed the control diet (P < 0.05). The result indicates that LEO can improve thyroid hormones and reduce the population of some potential pathogenic bacteria in broiler chickens' gut, resulting in better growth performance. Therefore, LEO can be recommended as much as 200 mg/kg of diet as an alternative to antibiotics in promoting the growth performance of broiler chickens.

Background: Herbal antioxidants have beneficial effects on health and performance. Meanwhile, medicinal plants and their derivations are utilized as growth and immunity promoter. Objectives: This research aimed to evaluate the effects of garlic powder and Satureja Khuzestanica essential oil (SKEO) on broiler performance, blood lipid profile, immune responses, intestinal microflora, and morphology. Methods: In total, 400 male chickens were randomly divided into five groups. Treatments included garlic powder (2 and 4%) and SKEO (400 and 500 mg/kg). Those in the control group received no intervention. On the 35th day of the experiment, the immune system of subjects was investigated, and at the end of the study, performance and blood lipid profile were analyzed. Also, the jejunal and ileal contents were separated to investigate the intestinal morphology and microflora. Results: Administration of herbal extracts was associated with improved performance, such as feed intake, BWG, and FCR (P < 0.05). Treatments could diminish the serum concentrations of lipid profile, including cholesterol, triglycerides, and LDL (P < 0.05). Meanwhile, the number of harmful intestinal bacteria was reduced by garlic (4% of diet) and SKEO (500 mg/kg) treatments (P < 0.05). Also, morphological characteristics of the intestine were improved (P < 0.05). Herbal plant supplement remarkably increased the villus length, villus length to crypt depth, and villus area (P < 0.05), but did not affect the immune responses (P > 0.05). Conclusions: This study demonstrated that dietary inclusion of herbal extracts is potentially an effective strategy for improving health and performance in broiler chickens.

Background: Poultry meat has high contents of polyunsaturated fatty acids that can cause off-flavors, off-odors, and reduce meat quality and shelf life. Replacement of phytogenic feed additives (PFAs) with chemical additives are highly considered, which reduces the potential risk of using poultry meat. Objectives: The current study aimed at determining the effects of hydroalcoholic extract of hogweed (Heracleum persicum) and anise (Pimpinella anisum L.) on broiler meat quality, immune responses, and intestinal microflora and morphology. Methods: The current study was conducted on 400 male Ross 308 chickens divided into five treatment groups, based on a completely randomized design with four replicates of 20 broilers. The control group was not fed any feed additives, whereas the other groups received 100 mg/kg of probiotics (Primalac (R)), 200 mg/kg of hogweed extract, 200 mg/kg of anise extract, and 200 mg/kg of oxytetracycline, respectively. On day 35 of chicken breeding, the immune responses were investigated. At the end of chicken breeding, meat quality was analyzed. Also, the jejunal and ileal contents were investigated in terms of intestinal morphology and microflora. Results: The findings indicated that the highest immune response belonged to the anise extract treatment. The best meat quality belonged to treatments with hogweed and anise extracts (P< 0.05). Also, the lowest numberof harmful intestinal bacteria belonged to hogweed extract treatment (P < 0.05). Morphological characteristics of the intestine improved under the effects of hogweed and anise extract treatments (P < 0.05). Conclusions: Therefore, inclusion of 200 mg/kg of hogweed and anise extracts in broiler diet is suggested to improve meat quality, immune responses, and intestinal morphology as well as reduce the number of harmful bacteria in the ileum.

The primary aim of this study was to assay the influence of pellet and mash diets supplemented with different levels of mesquite fruit on performance, energy and protein efficiency ratio, carcass characteristics, and intestinal morphology of broiler chickens. In this study, a total of 560 one-day-old (mixed sex) Ross 308 were distributed in a completely randomized design with 7 treatments, 4 replicates, and 20 chicks per replicate. Seven iso-caloric and iso-nitrogenous diets including a control diet (mash form without mesquite fruit), and pelleted and mash diets, consisting of three levels of mesquite fruit (3, 6, and 9%) were adjusted. The results exhibited that mesquite fruit contained a high percentage of dietary fiber. The results also showed that pelleted and mash diets containing different levels of mesquite caused a significant difference in feed intake, body weight gain, and feed conversion ratio in starter and grower periods. At the finisher period, there was a significant difference between the experimental treatments in terms of feed intake and body weight gain, but there was no significant difference in feed conversion ratio. Experimental treatments also caused a significant difference in energy and protein efficiency ratio in the starter and grower periods. Dietary treatments also exhibited a significant difference in the relative weight of breast and intestine, but no effect was found on carcass yield and relative weight of other internal organs. The findings revealed that pelleted and mash diets containing different levels of mesquite fruit did not show significant differences in morphological traits of the intestine. Taken together, our results suggested that, the use of mesquite fruit in pelleted diets can have positive effects on the performance of broilers.

This study aimed to investigate and compare nonlinear growth models (NLMs) with the predicted performance of broilers using an artificial neural network (ANN). Six hundred forty broiler chicks were sexed and randomly reared in 32 separate pens as a factorial experiment with 4 treatments and 4 replicates including 20 birds per pen in a 42-day period. Treatments consisted of 2 metabolic energy levels (3000 and 3100 kcal/kg), 2 crude protein levels (22 and 24%) and two sexes. Ten birds in each pen tagged and their weekly BW records were collected individually to evaluate the accuracy of predicted BW by ANN as an alternative to nonlinear regression models (Logistic, Gompertz, Von Bertalanffy, and Brody). Based on the goodness of fit criteria and error measurement statistics, the NLMs fitted the age-weight data better than ANN. The findings indicated that the performance prediction of broiler chicks using the Gompertz model (R-2 = 0.9989) was more accurate than other NLMs (R-2 = 0.9628 to 0.9988) and ANN (R-2 = 0.95839). Therefore, the application of the Gompertz model is suggested to predict the BW changes of Ross 308 broiler chicks over time.

An experiment was conducted to evaluate the effect of corn, wheat and barley-based diets supplemented with COMBO (R) multi-enzyme (an enzyme blend with 1000 active unit of phytase and 480 active unit of glycanase per gram in form of powder) on physiological response of chickens. Growth performance (gain, feed intake, and feed conversion), villus structure (villus height, villus width, crypt depth) and expression of glucose and peptide transporters (SGLT1, GLUT2, PepT1) and mucin producer (MUC2) genes in the small intestine of chickens were measured. One-day old Ross 308 mixed broiler chickens (n = 625) were allocated to 5 treatments with 5 replicates, using a completely randomized design (CRD), statistical plan. The control diet produced the greatest live weight (2251.26 g) and the lowest live weight (2015.77 g), was for birds on the wheat diet (P < 0.01). Cumulative feed intake and the mean daily feed intake was the greatest for the barley + enzyme treatment (4392.37 g) and the lowest (4164.21 g) for the wheat treatment (P < 0.01). The birds of control diet had the lowest feed conversion (1.90), but wheat and barley diets had the highest value (2.06). The smallest crypt depth in the jejunum (95 mu m) was associated with birds in the barley + enzyme treatment and the greatest crypt depth in the jejunum (116 mu m) belonged to birds on the wheat treatment. Wheat and barley diets resulted in a reduced villus height but supplementation with a multi-enzyme blend resulted in an increased the villus height (P < 0.01). Barley and wheat treatments had the greatest expression of glucose transporter (1.85 and 1.78 for SGLT1, 1.80 and 1.72 for GLUT2 respectively), peptide transporter (2.82 and 2.50 for PepT1) and mucin producer (1.38 and 1.48 for MUC2) genes in the small intestine (P < 0.01). In conclusion, using economical diets of wheat and barley complement to a corn-soy based diet without multi-enzyme is likely to have adverse effects on growth performance, villus morphology, and gene expression of nutrient transporters in the small intestine.

This study conducted to determine the effect of guanidino acetic acid consumption and management methods on the enhancement of aged rooster's reproduction. A total of 486 broiler breeders (Ross 308) were used in a 3 × 3 factorial experiment from week 41 to 62. Three levels as 0, 1200, and 1600 (mg/kg) of guanidino acetic acid, as a precursor of creatine (CreAmino) in combination with three management methods (no-management, spiking, and, intra-spiking methods) were evaluated. Quantitative and qualitative characteristics of sperm were determined every other week. At the end of the experiment, three roosters from each treatment were slaughtered and their testicles were evaluated for morphological and histological characteristics. The volume of sperm was a significant reduction in CreAmino (1200 mg/kg) + spiking. Sperm progressive motility reduced in CreAmino (1200 mg/kg) + nomanagement and CreAmino (1600 mg/kg) + intra-spiking. Also, CreAmino (1200 mg/kg) +no-management, increased the weight of the testicles. CreAmino (1600 mg/kg) + spiking showed the highest number of spermatozoa. The results showed that using 1600 mg CreAmino in combination with management methods, spiking and intra-spiking, can reduce and delay the occurrence of signs of aging in roosters. So, with using this level of CreAmino it is expected fertility in broiler breeder flock improved.

This experiment was conducted to determine the effects of active composition of different levels of garlic powder, clover powder and Satureja of Khuzestan (SK) essential oils on performance traits, fat reduction, carcass quality, meat quality and quantity, shelf-life, carcass traits, functional components, the concentration of the blood and immune system, metabolites and carcass antioxidant properties. A total number of 560 one-day old chicks of commercial Ross strain-308 were allocated in a completely randomized design with 7 treatments and 4 replicates of 20 birds per each replicate. Treatments were: 1. Control treatment (without additive). 2. Treatment containing garlic powder in two levels (2% and 4% of diet), 3. Clover powder treatment on two levels (5% and 10% food ration), and 4. Khuzestan satureja (Savory) essential oil in two levels (400 and 500 mg/kg). At 38 days of age, one bird from each replicate was randomly slaughtered and 30 cc blood and serum samples were taken from the jugular vein with syringes to evaluate the immune system, blood metabolites and anti-oxidant condition of broilers. The results showed that the impact of active constituents of garlic powder, clover powder and essential oils of savory for different treatments on the amount of dry matter, crude protein, crude fat and water holding capacity (WHC) and fat peroxidation of breast tissue at different times were significant (P<0.05). The results showed that the level of 10 percent clover powder relative to other treatments leads to reduction of WHC and the percentage of fat breast tissue. With regard to the different times of keeping meat Chest at freezer (0, 24 and 48 hours) the amount of breast tissue on fat peroxidation showed statistical differences. Production of Malonedialdehyde (MDA) in the breast meat did not show significant difference in experimental treatments (P<0.05). Preservation at different times showed significant impact on the amount of MDA production of breast meat (P<0.05). After a 48-hour freezing, the concentration of Malone aldehyde in breast meat of birds which received different levels of clover powder were less than the birds fed with essential savory and garlic powder (P<0.05).

This study was conducted to evaluate the feeding value of whole date palm (WDP) with and without enzyme supplementation on aged laying hen’s performance. Apparent metabolizable energy value of WDP was determined by the total collection method using ten adult leghorn cockerels. WDP was substituted with a corn-soybean meal basal diet at 40% level, and then the metabolizable energy of this experimental diet and basal diet was determined. After that, a total number of 256 Bovanz 95-week-old hens were randomly allocated into eight groups consisting of four replicates of eight birds each, based on a 4 × 2 factorial arrangement of the treatments. Eight iso-caloric and iso-nitrogenous experimental diets including one corn-soybean meal-based control diet and two, three, and four corn-soybean meal-based diets included 70, 140, and 210 g/kg ground WDP, respectively. Each of the diets supplemented with two levels of an enzyme (0.0 and 0.07 g/kg Natozim Plus). There was no significant difference in feed intake, feed conversion ratio, egg production, egg mass, eggshell thickness, and Haugh unit among the treatments (P > 0.05). However, yolk color score significantly decreased as dietary WDP level increased. The serum biochemical metabolites were not significantly affected by WDP and enzyme supplementation (P > 0.05). There were no significant differences in the relative weight of different organs except for abdominal fat. Our findings show that using WDP up to 21% of the diet was more economic and had no adverse effect on productive performance and serum metabolites of laying hens. However, WDP had an adverse effect on yolk color which can be ameliorated by carotenoid supplementation.

ABSTRACT The experiment was carried out in a completely randomized design (CRD) with 625 broiler chicks (Ross 308) for 5 repetitions (25 birds per each replicated) on the 5 treatments diet. Treatments included two different types of cereal grains (wheat, and barley) with or without an enzyme supplementation along with a corn-based diet as control group. The experimental diets were formulated to have similar contents of crude protein, metabolizable energy, total non-starch polysaccharides (NSP) and were fed in two periods of starter and grower. Experimental traits were consisted growth performance, ileal flora numeration, villus morphology in 3 parts of the intestine, digesta viscosity and pancreatic enzyme activity, and determining the gene expression level of glucose transporter (SGLT1) and mucin producer (MUC2) in the jejunum. Results indicated that inclusion of wheat and barley to corn-soy based diet with or without exo-enzymes blend on growth performance traits were significant (p<0.01). Feed intake and average daily gain in wheat diet was lower, conversely FCR was higher than other groups (p<0.01). Maximum microbial count were observed in wheat and barley diets and minimum were observed in enzyme supplemented diets respectively (p<0.01). Control group and enzyme supplemented diets had minimum counting of gram negative, coliform and clostridium, but maximum counting of lactobacilli and bifidobacter were observed in enzyme supplemented diets (p<0.01). Viscosity and activities of pancreatic a-amylase and lipase were significantly increased in chicks fed wheat and barley when compared to the control group fed on corn (p<0.01). Feeding wheat and barley diets reduced villus height in different parts of the small intestine when compared to those fed on a corn diet (p<0.01). Gene expression level of glucose transporter (SGLT1) and mucin producer (MUC2) in jejunum was significantly affected by the inclusion of wheat and barley to corn-soy based diet with or without exo-enzymes blend (p<0.01). In conclusion, the inclusion of wheat and barley to corn-soy based diet without enzyme supplementation has an adverse effect on growth, ileal microflora villi morphology, digesta viscosity, pancreatic enzyme activity, and gene expression level of nutrient transporters. However, enzyme supplemented to wheat and barley diets significantly improved those traits, and restored the negative effects.

An experiment was conducted to evaluate the effects of wheat and wheat bran diets with and without enzymes on growth performance and digestive characteristics of broilers.A total number of 625 one day old Ross-308 broiler chickens were allocated randomly to 5 treatments with 5 replicates, using a CRD statistical design.Treatments were control, wheat, wheat+ enzyme and wheat bran and wheat bran+ enzyme.Traits were growth performance, carcass characteristics, ileal microbial counting and measurement of gut acidity and viscosity.According to the results, effect of different treatments on all experimental traits at starter and finisher periods were significant (P<0.01).Minimum performance and maximum feed conversion rate at the whole rearing period belonged to treatments without enzymes.Supplementation of wheat and wheat bran with enzymes resulted in significant improvement of growth performance traits compare to control group (P<0.01).Effects of different treatments on carcass characteristics were significant (P<0.01).The highest carcass performances belonged to control group, but supplementation of wheat and wheat bran with enzymes resulted in significant improvement of carcass performances (P<0.01).Effects of different treatments on the intestinal microbial population were significant (P<0.01).Maximum undesirable bacteria were observed in treatments without enzymes, but maximum desirable bacteria were observed in treatments supplemented with enzymes.Digesta acidity and viscosity were lower and higher in the treatments without enzymes respectively (P<0.01).Supplementation of wheat and wheat bran with enzymes improved these digestive properties (P<0.01).In conclusion, inclusion of wheat and wheat bran in broiler corn-soy based diets were conceivable and supplementation of wheat and wheat bran diets improved the growth and carcass performances.

Introduction Corn has been recently used for producing ethanol fuel in the major corn-producing countries such as the US and Brazil. Recent diversion of corn for biofuel production along with the increased world's demand for this feedstuff has resulted in unprecedented rise in feed cost for poultry worldwide. Alternative grains such as wheat and barley can be successfully replaced for corn in poultry diets. These cereal grains can locally grow in many parts of the world as they have remarkably lower water requirement than corn. Wheat and barley are generally used as major sources of energy in poultry diets. Though the major components of these grains are starch and proteins, they have considerable content of non-starch polysaccharides (NSPs), derived from the cell walls (Olukosi et al. 2007; Mirzaie et al. 2012). NSPs are generally considered as anti-nutritional factors (Jamroz et al. 2002). The content and structure of NSP polymers vary between different grains, which consequently affect their nutritive value (Olukosi et al. 2007).Wheat and barley are generally used as major sources of energy in poultry diets. The major components of these grains are starch and proteins, they have considerable content of non-starch polysaccharides (NSPs), derived from the cell walls. NSPs are generally considered as anti-nutritional factors. The content and structure of NSP polymers vary between different grains, which consequently affect their nutritive value. The major part of NSPs in barley comprises polymers of (1→3) (1→4)-β- glucans which could impede growth factors and consequently carcass quality through lowering the rate and amount of available nutrients in the mucosal surface of the intestinal. Materials and Methods This experiment was conducted to evaluate the effect of corn and barley based diets supplemented with multi-enzyme on growth, carcass, pancreas enzyme activity and physiological characteristics of broilers. A total number of 375 one day old Ross-308 broiler chickens were allocated randomly to 3 treatments with 5 replicates using a CRD statistical design. Treatments were included control, barley and barley+ enzyme. The experimental diets were formulated to have similar contents of crude protein, metabolizable energy, total non-starch polysaccharides (NSP). Results and Discussion According to the results, effect of barley with or without enzyme on growth performance at starter, grower and the entire period and also on carcass characteristics, pancreas enzyme activity and measures of ileal acidity and viscosity at the age of 42 were significant (P

Physiological characteristics of meat-type chicken such as growth traits, serum biochemical metabolites and mineral concentrations, serum enzyme activity, and gut flora were studied by means of 625 day-old chicks which randomly assigned to five treatments with five replicates in a completely randomized design. Four different types of cereal-based diets (wheat, and barley with or without exogenous multi-enzyme supplement) were used as experimental groups and a corn-based diet was also considered to serve as control group. All diets had similar contents of crude protein, energy, and total non-starch polysaccharides (NSP). Results indicated that enzyme supplemented diets improved daily weight gain and feed conversion ratio than barley and wheat diets without enzyme significantly (P<0.01). Feeding wheat and barley diets decreased the serum cholesterol, triglyceride and glucose concentrations than control group significantly (P<0.01). Also using of these two type of cereals without enzyme supplementation resulted in reduced serum mineral concentration and undesirable effect on gut flora (P<0.01). Total gram negative bacteria of the ileum was decreased, however lactic acid and bifida bacteria population were increased by supplementation of wheat and barley diets with exogenous multi-enzyme significantly (P<0.01). The serum activities of α-amylase and lipase were increased in chicks fed wheat and barley diets when compared to the control group fed on corn diet, but enzyme supplementation significantly reduced the serum activities of α-amylase and lipase (P<0.01).

A study was conducted to investigate the effects of supplementation of dried tomato pomace (DTP) on growth performance, relative weights of viscera, serum biological parameters, antioxidant status, immune response, and bone composition of broilers exposed to a high ambient temperature. A total of 352 one-day-old male broiler chickens were randomly divided into four groups consisting of four replicates with 22 birds each. One group was reared under the thermoneutral zone and fed a corn–soybean meal basal diet. The other three groups were subjected to a cyclic heat stress from 29 to 42 days of age (34 ± 1 °C, 55 % RH, 5 h/day). These birds were fed corn–soybean meal basal diet or the same diet supplemented with 3 % DTP (420 mg lycopene/kg diet) or 5 % (708 mg lycopene/kg diet) of DTP. Blood samples were collected on days 28 and 42, and the birds were slaughtered at the same times. Supplementation of 5 % of DTP increased body weight and production index and decreased feed conversion ratio during 1–28 days of age. On day 28, the broilers supplemented with 5 % DTP had lower serum triglycerides and higher high-density lipoprotein (HDL) cholesterol concentration than those on the other dietary treatments. The activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD) were higher and the concentration of malondialdehyde (MDA) was lower in the broilers fed 5 % TP than those of the broilers fed other diets at 28 days of age. The effects of heat stress (HS) were impaired body weight, enhanced serum activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lipase, and MDA concentration while reducing the activities of GPx and SOD. Dried tomato pomace supplementation did not influence growth performance under HS but ameliorated the negative effects of HS on the serum enzyme activities, GPx activity, and lipid peroxidation. Heat stress did not change the relative weights of the lymphoid organs but reduced the total and IgG titers for secondary antibody response to sheep red blood cells and titer against Newcastle disease virus and increased the heterophil/lymphocyte ratio. The supplementation with 5 % of DTP completely alleviated the negative effects of HS on immune responses. The ash, Ca, and P contents of the tibia bone were decreased under HS. The ash and Ca contents of the tibia were not significantly different between thermoneutral and heat-stressed broilers supplemented with 5 % DTP. In conclusion, dietary supplementation of DTP, particularly 5 % DTP, to broiler diet attenuated the detrimental effects of HS on the activities of serum enzymes, oxidative status, immune response, and bone composition.