Background/Objectives: The process of cooking food can result in alterations to its nutrient composition due to changes in water content and the destruction or loss of certain micronutrients that occur in response to heat. This study examined the impact of diverse cooking techniques, namely grilling, microwave, and steam, on the macronutrients and minerals of vegetables commonly utilized in Italian cuisine (two varieties of zucchini, eggplants, and potatoes). Methods: The proximate composition was determined according to the Association of Official Analytical Chemists (AOAC) methods. The content of the minerals (Ca, K, P, Mg, Na, Fe, Zn, and Mn) was determined via ICP plasma after liquid washing. Results: Regarding macronutrients, the results revealed a notable difference in the carbohydrate profiles, whereas mineral retention demonstrated considerable heterogeneity. Some minerals, such as Na, Ca, Mn, and Fe, were found to be more prone to significant increases or losses. Moreover, the true retention factor (TR) calculations indicated that microwave cooking resulted in higher retention compared to the other methods for zucchini, while grilling demonstrated higher TR than microwave cooking for eggplants. Potatoes exhibited lower TR values than the other vegetables and their steaming resulted in higher retention than microwave cooking for K, P, Fe, and Zn. Conclusions: The results confirm the heterogeneous behaviors of minerals in commonly consumed Italian vegetables subjected to different cooking methods. The data underscore the need for additional research to understand the effects of heat treatments on mineral profiles and to determine specific retention factors linked to various cooking techniques. The significant gap between “true” and “apparent” retention factors, caused by changes in water content during cooking, highlight the need for new experimental data to update and enrich the existing literature on this topic.

A standardized classification and description system (coding) of food products allows the analysis of data originating from several sources. The data harmonization process about food composition depends on systematic food description that is indispensable for the acquisition, analysis, and sharing data and information. The availability of well-developed data banks can ensure the efficient management of heterogeneous data, useful for scientific studies and strategic decisions. The rise of harmonized composition data for foods plays a key role in understanding the relationship between human health and diet. Systematic classification and description of food products are used to analyze dietary intake. "Specialty oils", also promoted as "Gourmet" oils, are attracting the attention of consumers for their high amounts of nutritional and nutraceutical components with health-promoting properties. This study aims to codify 50 samples of some vegetable oils, currently sold in physical and online stores in Italy, by applying the two most common classification and description systems: LanguaLTM and FoodEx2. [GRAPHICS]

Sea cucumbers are marine invertebrates with important ecological roles and a food delicacy with high market value. This study investigated the compositional profile and nutritional features of two Mediterranean sea cucumbers, Holothuria polii and Holothuria tubulosa, harvested in Adriatic Sea and Ionian Sea, Southern Italy. The macronutrients were determined by AOAC methods. The minerals were determined by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) and fatty acids by Gas Chromatography-Mass Spectrometry/ Flame Ionization Detector (GC-MS/FID). Total mercury content was analyzed by Thermal Decomposition-Amalgamation-Atomic Absorption Spectrophotometry. Results showed that holothurians from the two basins are good protein (average 38-60 % dry mass) and mineral (32-37 % dry mass) sources, have a very low lipid content (1-3.7 % dry mass) and negligible mercury levels (0.016-0.038 mg kg-1 dry mass). H. polii showed a higher nutrient density, especially regarding minerals, with calcium, sodium and magnesium as the major ones. The analysis of fatty acid profiles enabled the identification of specific markers, reflecting habitat characteristics, seasonality, and dietary attitudes of holothurians. High percentages of arachidonic acid (47-65 % of total polyunsaturated fatty acids) and remarkable levels of branched-chain (6-9 % of total fatty acids) and odd-chain saturated fatty acids (1.7-3.8 % of total fatty acids), trophic markers of benthic food webs and sediment microbiota, were characterizing traits. Principal Component Analysis showed a neat discrimination of holothurians based on the species and harvesting site. Results underscore the nutritional properties of Mediterranean holothurian as part of a balanced diet, highlighting their potentialities as sources of molecules of nutritional and nutraceutical interest.

Pimenta dioica (L.) Merr., known as allspice, is a Caribbean tropical tree used for centuries by the local tribes in traditional cuisines and folk medicine. Due to its chemical composition, it has been investigated for related biological activities and potential benefits. Studies have reported interesting activities such as antibacterial, hypotensive, antineuralgic, analgesic, etc. Moreover, gallic acid, eugenol, and (poly)phenols present in extracts of the plant have been studied for potential antitumour activity. P. dioica is also used in dairy, perfumery, cosmetic, and pharmaceutical industries. The aim of this chapter is to give an updated summary of the main features and potential of allspice. In addition to the botanical and geographical aspects, we have explored the possible beneficial effects on human health due to the presence of bioactive compounds, indicating their traditional use in food and medicinal preparations as well as proposing possible future applications.

Ready-to-eat (RTE) and ready-to-heat (RTH) dishes are food items that help save time, physical energy, and mental effort in all food-related activities. Convenience of use, variability of supply, and adaptability to different consumption occasions have led to an increase of acceptance among consumers through the years. Specialized databases can help in this context, where food composition databases can provide information and data to create sustainable nutritional models by reducing the now growing number of chronic diseases. This paper aims at developing a database of LanguaLTM and FoodEx2 codes of 50 food preparations and ready-to-eat dishes designed for consumption outside the home. LanguaLTM, as well as FoodEx2, are classification and description systems for indexing, in the sense of a systematic description, of foods based on a hierarchical model (parent–child relationship), thus facilitating the international exchange of data on food composition, consumption, assessing chronic and/or acute exposure to a certain agent, and not least the assessment of nutrient intake. The database, here presented, consists of the codes of fifty ready-to-eat products present on the market in Italy, obtained by using the two mostly commonly used and widely recognized coding systems: LanguaLTM and FoodEx2. This database represents a tool and a guideline for other compilers and users to apply coding systems to ready-to-eat products. Moreover, it can be represented a resource for several applications, such as nutritional cards, nutritional facts, food labels, or booklet and brochures for promotion of food products, to be used at health and food nutrition interface, useful for consumers, dieticians, and food producers.

In recent decades, scarcity of available resources, population growth and the widening in the consumption of processed foods and of animal origin have made the current food system unsustainable. High-income countries have shifted towards food consumption patterns which is causing an increasingly process of environmental degradation and depletion of natural resources, with the increased incidence of malnutrition due to excess (obesity and non-communicable disease) and due to chronic food deprivation. An urgent challenge is, therefore, to move towards more healthy and sustainable eating choices and reorientating food production and distribution to obtain a human and planetary health benefit. In this regard, legumes represent a less expensive source of nutrients for low-income countries, and a sustainable healthier option than animal-based proteins in developed countries. Although legumes are the basis of many traditional dishes worldwide, and in recent years they have also been used in the formulation of new food products, their consumption is still scarce. Common beans, which are among the most consumed pulses worldwide, have been the focus of many studies to boost their nutritional properties, to find strategies to facilitate cultivation under biotic/abiotic stress, to increase yield, reduce antinutrients contents and rise the micronutrient level. The versatility of beans could be the key for the increase of their consumption, as it allows to include them in a vast range of food preparations, to create new formulations and to reinvent traditional legume-based recipes with optimal nutritional healthy characteristics.

Purslane (Portulaca oleracea L.) has always been considered a plant with excellent qualities; in fact, it has been consumed for centuries as an edible plant but also used in folk medicine. This work aims to give an updated shot of the main features and potentialities of purslane. A literature quantitative research analysis was carried out. Besides the botanical and geographical aspects, we have explored the nutritional properties and possible beneficial effects on human health due to the presence of bioactive compounds, indicating their traditional use in food and medicine preparation as well as proposing possible future applications. Purslane is a rich source of vitamins, flavonoids, organic acids, lignans, alkaloids, terpenoids, cerebrosides, sterols and minerals; in particular, it is considered a good vegetable source of unsaturated fatty acids, especially linoleic and alpha-linolenic acids. Due to its profile of bioactive components, several potential beneficial effects on humans have been reported, i.e. hepatoprotective, neuroprotective, anti-inflammatory, antimicrobial and antioxidant. Purslane finds several applications: enhancing the nutritional and sensory properties of bread, stabilising yoghurt, improving fish sausages’ quality and shelf-life, acting as a natural additive due to its antibacterial, antifungal, and antioxidant properties. Additionally, it can be utilised for phytoremediation and recycling drainage water in saline environments.

Spices have been used for thousands of years in food preparation, giving pleasant flavours and colours. Spices help to preserve food and have been also used as medicines for their potential beneficial effects. Asafoetida, an oleo-gum-resin obtained from the stems of Ferula, is used as a flavouring agent in food and as a traditional medicine for several diseases in many parts of the world. The phytochemicals present in the genus Ferula, such as coumarins, terpenes, sulphur-containing compounds, phytoestrogens, flavonoids, etc., confer on the Ferula plants beneficial properties. Recent studies have shown several potential benefits including neuroprotective, antioxidant, hypotensive, hepatoprotective, antimicrobial, anticarcinogenic, anticancer, anticytotoxicity, and antiobesity properties. This chapter aims at giving an updated summary of the main features and potential benefits of asafoetida. In addition to the botanical and geographical aspects, we have explored the possible beneficial effects on human health due to the presence of bioactive compounds, indicating their traditional uses in food and medicine preparation as well as in proposing possible future applications.

Rapeseed meal (RSM), a by-product of rapeseed oil extraction, is currently used for low-value purposes. With a biorefinery approach, rapeseed proteins may be extracted and recovered for high-end uses to fully exploit their nutritional and functional properties. This study reports the application of RSM protein isolate, the main output of a biorefining process aimed at recovering high-value molecules from rapeseed meal, as a supplement to texture-modified (TM) food designed for elderly people with mastication and dysphagia problems. The compositional (macronutrients by Official Methods of Analyses, and mineral and trace element profiles using Inductively Coupled Plasma Optical Emission Spectrometry ICP-OES), nutritional and sensory evaluations of TM chicken breast, carrots and bread formulated without and with RSM protein supplementation (5% w/w) are hereby reported. The results show that the texture modification of food combined with rapeseed protein isolate supplementation has a positive impact on the nutritional and sensory profile of food, meeting the special requirements of seniors. TM chicken breast and bread supplemented with RSM protein isolate showed unaltered or even improved sensory properties and a higher nutrient density, with particular regard to proteins (+20–40%) and minerals (+10–16%). Supplemented TM carrots, in spite of the high nutrient density, showed a limited acceptability, due to poor sensory properties that could be overcome with an adjustment to the formulation. This study highlights the potentialities of RSM as a sustainable novel protein source in the food sector. The application of RSM protein proposed here is in line with the major current challenges of food systems such as the responsible management of natural resources, the valorization of agri-food by-products, and healthy nutrition with focus on elderly people.

Nowadays, modern agriculture looks for valid, sustainable, and green alternatives that are able to improve and maintain soil quality and fertility over time. Recycling organic waste as fertilizer is one of the strategies for sustainable production. Recently, the use of new products derived from compost, such as compost tea (CT), is increasing due to their positive effects on crops. This perspective wants to give an updated shot at the effect of compost tea in horticulture. In addition to the classification of compost tea, with a focus on production procedures and composition, the possible effects they have both on the control of phytopathogens in horticulture and the influence they can have on the content of bioactive molecules and nutrients were highlighted. It is interesting to note that compost teas can have an effect on the final content of micro and macronutrients, thus improving the nutritional qualities and also increasing the content of bioactive compounds that may play a role in maintaining and improving human health. The combined use of compost tea with other treatments is being explored as a promising and innovative direction.

Chlorophyll pigments are thought to be responsible for the highly appreciated green color of unfermented Castelvetrano-style table olives, but no studies have considered the effects of a controlled addition of copper during storage or packaging at the industrial level. For this purpose, chlorophyll derivatives were analyzed in Nocellara cultivar table olives debittered industrially using the Castelvetrano method, via means of HPLC and MS analyses, following the addition of copper in alkaline brines stored at 4 °C for 3 months in 220 L barrels, and during the subsequent storage in acid brines in commercial 400 g packages at 4 °C for up to 18 months. The presence of copper in storage or in packaging brines both contributed significantly to maintaining the green color of the olives, which was associated with a specific pattern of chlorophyll derivatives, as evidenced by principal component analysis. Notably, re-greening was rapidly achievable also for olives that had yellowed for 18 months at a copper concentration below the limit of EU legislation. Finally, by means of PCA, we also demonstrated that a short-term thermic treatment can work as an accelerated predictive tool in determining the fate of chlorophyll derivatives.

Folate is a fundamental vitamin for human health in prevention of many diseases; however, unfortunately its deficiency is widespread, so a greater availability of folate rich foods is desirable. The aim of this study was to design new cereal products naturally enriched in folate using barley flour from pearling as ingredient. Folate content of unfortified and fortified commercial grain-based products was considered to identify the best ingredients for new formulation and for folate content comparisons. Nineteen Italian barley cultivars were evaluated for their folate content and Natura was chosen for its highest folate levels = 69.3 μg/100 g f.w. Application of pearling gave a by-product flour with a high folate level: 221.7 ± 7.0 μg/100 g; this flour was employed to design pasta and biscuits naturally enriched in folate: 87.1 μg/100 g and 70.1 ± 3.7 μg/100 g f.w., respectively. Folate content of new products is higher than commercial samples: 39.2 μg/100 g in refined pasta, 60.4 μg/100 g in wholemeal pasta, 62.1 μg/100 g in fortified biscuits and 10.4 μg/100 g in unfortified ones. Enriched pasta had higher folate retention (68.5%) after cooking compared to the fortified one (27.8%). This research shows promising results concerning the pearling technique to design new cereal products naturally enriched in folates.

Nutritional and sensory quality of wild rocket leaves (Diplotaxis tenuifolia L., cv. Grazia) subjected to commercial processing and packaging were monitored for up to 7 days of cold storage and compared with the quality of unprocessed and unpackaged leaves from the same harvest. Nutritional quality was assessed by determining total phenolics, vitamin C and folate content. Sensory quality was assessed by quantitative descriptive analysis, combined with analysis of volatile organic compounds (VOCs). No change in vitamin C and folate content was observed in processed rocket leaves up to 7 days of cold storage, whereas unprocessed leaves showed a marked loss of both of them at the end of the storage time (52% and 71%, respectively). Total phenol content decreased during cold storage in both rocket leaves samples, but at a lower extent in the processed leaves. While most sensory attributes showed significant degradation during storage in both processed and unprocessed leaves, at the end of the storage time processed leaves had better retained some appearance (colour, turgidity, integrity), flavour (bitterness and bitter persistence) and texture (firmness and chewing consistency) attributes of the fresh leaves. A marked decrease during storage of some potent key odorants, such as (Z)-3-hexenal, (E)-3-hexenal, as well as 1-penten-3-one, corresponded to the weakening of fresh herbaceous/green sensory notes and pungency in both rocket leaves samples. Commercial processing and packaging operations proved to be effective at better preserving nutritional and sensory quality of wild rocket leaves and, thus, at prolonging their shelf life.

Garlic (Allium sativum L.) is one of the most important food products in the world and an ancient and widespread medicinal herb. It is rich in minerals and vitamins, which are both essential nutrients for human health. This study was carried out on four traditional landraces of garlic: Rosso di Sulmona, Rosso di Proceno, Bianco Piacentino, and Rosso di Castelliri, all cultivated with the same agricultural practices in two different areas of the Lazio region. The aim was to study the effects of both production soil and genetic characteristics on the concentrations of certain micronutrients in these garlic bulbs. The content of minerals and trace elements (Ca, K, P, Mg, Na, Fe, Zn, Cu, Mn) was determined via ICP plasma after liquid washing. Water-soluble vitamins (riboflavin, niacin, thiamine, B6, vitamin C) were evaluated using an HPLC and UV detector. Among the minerals identified, potassium was most represented, with a value ranging between 645 and 1057 mg/100 g d.w., whose content is influenced by the interaction between cultivars and cultivation area, suggesting that pedoclimatic and genetic characteristics determine this content. Among vitamins, vitamin C was found to be most represented, with an amount that varied from 9.7 to 15.6 mg/100 g f.w. and did not depend on the cultivar or the geographical area. Among the B vitamins, the most abundant was vitamin B6, the maximum content of which was found in the Rosso di Castelliri cultivar grown in Viterbo (2.04 mg/100 g f.w.). Its concentration is influenced by the cultivar and the soil and their interaction. The results obtained showed good levels of essential micronutrients and highlighted that for some of them, the effect of soil typology is expressed in the presence of specific characteristics linked to the genotype.

Rapeseed meal (RSM), a by-product of oilseed extraction connected to the agri-food and biofuel sectors, is currently used as animal feed and for other low-value purposes. With a biorefinery approach, RSM could be valorized as a source of bio-based molecules for high-value applications. This study provides a chemical characterization of RSM in the perspective of its valorization. A qualitative study of main functional groups by fourier transform infrared (FTIR) spectroscopy was integrated with a chemical characterization of macronutrients, minerals by inductively coupled plasma optical emission spectrometry (ICP-OES), phenolic acids and lipid components by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), HPLC-diode-array detector (HPLC-DAD) and gas chromatography-mass spectrometry/flame ionization detector (GC-MS/FID). The study, conducted on different lots of RSM collected over a one-year period from an oil pressing factory serving a biofuel biorefinery, highlighted a constant quality over time of RSM, characterized by high protein (31–34%), fiber (33–40%) and mineral (5.5–6.8%) contents. Polyphenol extracts showed a significant antioxidant activity and a prevalence of sinapic acid, accounting for more than 85% of total phenolic acids (395–437 mg kg−1 RSM). Results highlight the potentialities of RSM for further valorization strategies that may lead to the creation of new cross-sector interconnections and bio-based value chains with improvement of the economics and sustainability of the bioeconomy sectors involved.

First-generation biofuel biorefineries may be a starting point for the development of new value chains, as their by-products and side streams retain nutrients and valuable molecules that may be recovered and valorized for high-value applications. This study provides a chemical characterization of post-fermentation corn oil and thin stillage, side streams of dry-grind corn bioethanol production, in view of their valorization. An overall long-term study was conducted on the two co-products collected over 1 year from a bioethanol plant. Water content, acid value, sedimentation, mineral composition, and fatty acid profiles were analyzed on post-fermentation corn oil. Results highlighted that its acid value was high (19.72–24.29 mg KOH/g), indicating high levels of free fatty acids, but stable over the year due to standardized operating conditions. The fatty acid profile was that typical of corn oil, with a prevalence of linoleic (54–59% of total fatty acids) over oleic (23–27%) and palmitic (12–17%) acids. Macronutrients, fatty acid, and mineral profiles were investigated in thin stillage. Results revealed the acidic pH (4.05–4.68) and high dilution (90–93% water) of this side stream. The dry mass was composed of fats (19–30%), proteins (8.8–12.8%), ash (8.7–9.5%), and fiber (7.3–9.8%). The concomitant presence of a variegate complex of molecules of nutritional interest in corn bioethanol co-products, with several potential high-value market applications, make the perspective of their recovery a promising strategy to create new cross-sector interconnections according to circular economy principles.

The present study evaluated the content of glycoalkaloids (α-chaconine and α-solanine) in the edible portion of six hybrid potato clones coming from the Italian potato breeding programme focused on resistance against the potato tuber moth (Phthorimaea operculella Zeller). To evaluate the influence of agro-climatic conditions on glycoalkaloid content, the potato clones were grown for two consecutive years in the four main potato production areas in Italy. The effect of genotype, cultivation area and year, and the interaction among these factors, were studied. A large variability in glycoalkaloid concentration was found (from 16.8 to 227 mg kg−1). Among the new potato clones, three (ISCI 181/10–4, ISCI 182/10–1 and ISCI 232/12–1) had low glycoalkaloid concentrations. Only one potato clone (ISCI 207/11–1) was over the safety limit of 200 mg kg−1. Genotypic variation was the main factor influencing glycoalkaloid accumulation, although the influence of agro-climatic conditions was also highlighted.

Four popular dishes belonging to Italian cuisine and widely consumed in the country were experimentally prepared in a dedicated lab-kitchen following a validated and standardized protocol. This study provides their antioxidant properties evaluating the contribution of extractable and non-extractable bioactive compounds, and identifying the assessment of interactions between their natural active compounds and the food matrix. Ferric reducing antioxidant power (FRAP) values in aqueous-organic extract ranged from the highest antioxidant activity in torta di mele (10.72 µmol/g d.m.) to that in besciamella (2.47 µmol/g d.m.); in residue, pasta alla carbonara reached the highest value (73.83 µmol/g d.m.) following by that in pasta alla amatriciana (68.64 µmol/g d.m.). Total polyphenol content (TPC) ranged in aqueous-organic extracts between 36.50 and 64.28 mg/100 g d.m. and in residue from 425.84 to 1747.35 mg/100 g d.m. Our findings may contribute to the updating of the Italian Food Composition Database, by providing for the first time a value for the antioxidant properties. This could contribute to encourage the consumption of recipes rich in key nutrients and bioactive molecules. This information is useful and important for determining the association between diet and a healthy status.

Biomass from five microalgal species, Porphyridium cruentum, Isochrysis galbana , Phaeodactylum tricornutum, Tetraselmis suecica and Nannochloropsis gaditana, produced at an industrial plant in outdoor photobioreactors, was studied with the aim to evaluate their suitability to the food and nutraceutical sectors. Microalgal biomass was analyzed for proximates, nonprotein nitrogen, energy, fatty acids, minerals, trace elements and mercury contents. Proximate analyses showed wide differences among microalgal species, in accordance with their different taxonomic position, especially as regards protein (19.6–33.2% dry mass), carbohydrate (15.9–42.2% dry mass) and lipid (5.7–31.1% dry mass) contents. All species proved to be a good source of minerals and trace elements and of polyunsaturated fatty acids (47.4–59.1% of total fatty acids) with varying profiles. N-3 fatty acids were prevalent in P. tricornutum , N. gaditana , I. galbana and T. suecica (32.6–36.4% of total fatty acids), whereas n-6 fatty acids, mainly arachidonic (C20:4) and linoleic (C18:2) acids, were prevalent in P. cruentum (43.3% of total fatty acids). N. gaditana , P. tricornutum , and P. cruentum were rich in eicosapentaenoic acid (36.0, 29.3%, and 15.9% of total fatty acids, respectively), while I. galbana was a good source of stearidonic (C18:4, 12.2% of total fatty acids) and docosahexaenoic (9.0% of total fatty acids) acids, undetectable or present at low levels in the other species. I galbana and T. suecica showed also high percentages of α-linolenic acid (C18:3, 12.2%–15.7% of total fatty acids). All microalgae were characterized by good nutrient contents and confirmed to be potentially valuable ingredients for nutritional or nutraceutical purposes.

An integrated and multidisciplinary system of analysis, using innovative and emerging technologies, combined with chemometric data evaluation, is becoming a valuable tool for food analysis. The main lines of this approach and the exploitation of innovative possibilities applied to milk products are reviewed and discussed. Special attention is paid to spectroscopic techniques combined with multivariate statistical data analysis. Moreover, a case study of a milk-based dish is reported: the IR spectroscopic characterization and assignment of the main bands of Bechamel sauce is shown. This can serve as a basis for developing multivariate calibration models for useful applications in order to collect information having specific required characteristics, e.g. for discrimination of products and the investigation of fraud. The main perspective is the increased awareness of the importance of sharing data and the creation of an integrated system for calibration and standardization of milk product spectra in the Multicentre Research Network. It aims at making the analysis faster to ensure economic and health safety in a global context.