The market of plant-based soft drinks responds to the growing demand for functional products that combine pleasant sensory properties with health benefits. This article describes the impact of formulation factors (raw material, sweetener, ascorbic acid) on the quality, functional properties, and stability of plant-based soft drinks during storage. The study featured plant-based semi-finished products combined into 12 beverage formulations. They were subjected to standard physicochemical, microbiological, and spectrophotometric analytical methods. The sensory assessment involved a five-point scale. The statistical data processing relied on the analyses of variance and correlation. The beverages varied in three factors. The semi-finished plant material (coffee infusions, cinnamon, Rhodiola rosea CO2-extract) determined the content of solids, phenolic compounds, flavonoids, color coordinates ( 2 p η = 89.7–99.6%), QMAFAnM ( 2 p η = 26.5%), and sensory properties ( 2 p η = 50.2–52.2%). The sweetening agent (sucrose/sucralose) affected the content of solids and sucralose ( 2 p η = 100.0 %), QMAFAnM ( 2 p η = 42.3%), flavonoids ( 2 p η = 24.0%), and the color indicators L*, a*, H° ( 2 p η = 41.9–56.2%). Ascorbic acid ( 2 p η = 99.9%) ensured at least 15 mg of vitamin C per 100 cm3 of the finished product. It also affected the b*, C* indicators and solids ( 2 p η = 17.4–26.5%). The beverages retained acceptable sensory profile, functional properties, and microbiological safety over 7 days of storage. The experimental sucralose-based beverages proved to be low-calorie functional drinks able to ensure glycemic control. A 200 cm3 serving, fortified with ascorbic acid, delivered 30% of the recommended daily intake of Vitamin C. These findings contribute to the diversification of the functional food market.

[This retracts the article DOI: 10.1016/j.heliyon.2020.e03299.].

Soil health is a critical determinant of agricultural productivity and environmental sustainability. Traditional assessment methods often fail to provide a comprehensive understanding of soil microbial communities and their functions. This study addresses this challenge by employing metagenomic techniques to assess the functionality of soil microbiomes in Russian black soil, renowned for its high fertility. We utilized shotgun metagenomic sequencing to analyze soil samples from Western Siberia subjected to different degrees of agro-soil disturbance. We identified functional genes involved in carbon (accA, argG, acsA, mphE, miaB), phosphorus (phoB, ppa, pstB, pnp, phnJ), and nitrogen (queC, amiF, pyrG, guaA, guaB, napA) metabolic pathways and associated with changes in microbial diversity in general and higher representation of certain bacterial species - Bradyrhizobium spp. Results demonstrated significant differences in microbial composition and functional potential between tillage treatments. No-Till technology and conventional tillage practices promoted beneficial microbial communities and enhanced soil health compared to long-term fallow soil. This work underscores the potential of metagenomic analysis in providing a comprehensive understanding of soil health, marking a significant advancement in the field.

Monitoring wildlife exposure to biological hazards is a critical component of the wildlife risk assessment. In this study 38 hair samples were collected from 8 different species from ten districts of Russian Far East and Siberia and analysed for the presence of organochlorine pesticides (OCP). 50

This study investigated the combined effects of deltamethrin and T-2 toxin on animals, utilizing bentonite as a prophylactic agent. The research was conducted on white rats, which were orally administered the toxins for 20 consecutive days. Subsequently, clinical, hematological, and biochemical parameters were examined to assess the impact of the toxins on the animals. Based on research results, it has been established that the combined effect of deltamethrin and T-2 toxin with repeated administration is characterized by more severe clinical, hematological and biochemical changes than with separate exposure to toxicants. Accompanied by a decrease in the number of leukocytes, erythrocytes and hemoglobin, a decrease in total protein, glucose and cholinesterase activity. Adding bentonite to the feed as a prophylactic agent at a dose of 2% of the diet has a preventive effect in case of combined poisoning with deltamethrin and T- 2 toxin, and helps to normalize the clinical, hematological and biochemical parameters of animals.

Abstract Particles in welding fumes (WFs) generated through arc welding operations pose serious health concerns to the welders through their exposure to heavy metals. In this study, the influence of different covering types of industrial electrodes (rutile, basic, acid, rutile-cellulose) on the particle size distribution, morphology, and elemental composition of particles of welding fumes was investigated. Quantitative analysis was conducted in order to determine the distribution of particles with diameters of 10μm, or less, (PM10 fraction) of the WFs within the workplace, followed by the comparison of the results with the current international normative documents on the maximum permissible concentration of the PM10 fraction in the working zone air. The most hazardous types of electrode coverings were determined based on the dispersion, chemical composition, and concentration of formed particles of the PM10 fraction in space and time. The dependence of the particle size distribution time of the WFs in the working zone was identified for a basic covered industrial electrode. The maximum sizes of WF particles were reported for operations held at 100 A with electrodes having the rutile-cellulose type of covering, and at 150 A having the basic type of covering. A concentration of 0.05 mg/m3 for the PM10 fraction of WFs in the workplace was achieved after 1 hour of the welding machine operation at a current equal to 100 A. Thus, the results of the characterization of WFs demonstrate the risks of the arc welding process to human health and stress the need for their control and mitigation. According to the results of the study, a proportional relationship between the average particle diameter and the WF particle distribution period in the workplace has been demonstrated, which has been particularly evident from the height of the WF particles. 3D modeling of the dispersion of WF particles during welding arc operations proved to be a suitable method for their characterization.

A promising and efficient approach to obtaining a genetically pure and identical plant to a given explant is through microclonal propagation. This method pre-determines the reproduction of plants by managing, controlling, and regulating the parameters of external and internal conditions that influence the accelerated reproduction of cultivated plants. The main methods for implementing the accelerated reproduction of cultivated plants are propagation in a closed external environment using specialized nutrient solutions and the use of bioreactors with temporary immersion of plants. The process of reproducing cultivated plants, specifically potatoes, using temporary immersion bioreactors allows for the use of automated monitoring and control systems based on robust control systems. This increases productivity and ensures the quality of the reproduced explants. Research has led to the development of experienced statisticians who have established the impact of individual parameters on the management of plant reproduction on their quality indicators. The reliability of the study is ensured by a large sample size (over 200 objects), parametric statistics, and the use of group theory to estimate plant reproduction parameters.

The article presents a review of literature on bioactive substances and innovative methods of extraction of biologically active substances from plant raw materials, discusses the need to develop new extraction methods to preserve the structural and functional properties of the extracted compounds. The study includes analysis of recent publications on extraction methods and bibliometric mapping using VOSviewer software. The authors review various methods including supercritical fluid extraction, fermentation, ultrasonic and microwave processing. Special attention is given to combined methods such as enzyme extraction using ultrasound and microwaves. The advantages and disadvantages of new extraction methods, as well as combinations of techniques in terms of increasing the potential for extraction of bioactive compounds from plant raw materials are determined. The work is intended to stimulate interest in the development of new extraction methods and to promote research in this field.

Despite great advances in the treatment of oncological diseases, the development of medical technologies to prevent or reduce complications of therapy, in particular, those associated with surgery and the introduction of antibiotics, remains relevant. The aim of this study is to evaluate the effectiveness of the use of autoprobiotics based on indigenous non-pathogenic strains of Enterococcus faecium and Enterococcus hirae as a personalized functional food product (PFFP) in the complex therapy of colorectal cancer (CRC) in the early postoperative period. A total of 36 patients diagnosed with CRC were enrolled in the study. Study group A comprised 24 CRC patients who received autoprobiotic therapy in the early postoperative period, while the control group C included 12 CRC patients without autoprobiotic therapy. Prior to surgery and between days 14 and 16 post-surgery, comprehensive evaluations were conducted on all patients, encompassing the following: stool and gastroenterological complaints analysis, examination of the gut microbiota (bacteriological study, quantitative polymerase chain reaction, metagenome analysis), and analysis of interleukins in the serum. Results: The use of autoprobiotics led to a decrease in dyspeptic complaints after surgery. It was also associated with the absence of postoperative complications, did not cause any side effects, and led to a decrease in the level of pro-inflammatory cytokines (IL-6 and IL-18) in the blood serum. The use of autoprobiotics led to positive changes in the structure of escherichia and enterococci populations, the elimination of Parvomonas micra and Fusobacterium nucleatum, and a decrease in the quantitative content of Clostridium perfringens and Akkermansia muciniphila. Metagenomic analysis (16S rRNA) revealed an increase in alpha diversity. Conclusion: The introduction of autoprobiotics in the postoperative period is a highly effective and safe approach in the complex treatment of CRC. Future studies will allow the discovery of additional fine mechanisms of autoprobiotic therapy and its impact on the digestive, immune, endocrine, and neural systems.

In order to study the effect of a number of 6-substituted uracil derivatives on bovine embryo lung cells, experiments were conducted. According to the study, the effect of the studied substances on the cultural and morphological properties of cell was determined by taking into account their viability and proliferation index. In %, it was calculated that viability was determined by the ratio of living cells to their total number. The proliferation index was determined as the ratio of the number of proliferating cells to the number of seeded cells. At 0.1 mM concentration of the compounds studied, a decrease in LEK cell viability was recorded compared to the control group, but this was only the case for compound No. 2. When other compounds were used at doses of 0.1 mM, LEK cell viability increased by 3.0-7.0% compared to controls. Increasing the dose of the compounds studied to 1.0 mM results in a 10.0% decrease in survival in the presence of compounds No. 3 and 6, 16.0% in the presence of compounds No. 2 and 7, 19.0% in the presence of compound No. 4 and 25.0% in the presence of compounds No. 8 and 9.

Identification of anthocyanidins was carried out in five samples of anthocyanins obtained under different extraction conditions. It has been shown that anthocyanins are most fully extracted from black currant by an aqueous solution at temperatures of 70°С for 30 minutes and 100°C for 5 minutes, treatment in an ultrasonic bath for 30 minutes and in an ultrasonic homogenizer for 15 minutes. Eight anthocyanidins were identified in the samples of anthocyanins isolated by various methods. The most common were five anthocyanidins - these are cyanidin-3-glucoside, cyanidin-3-rutinoside, delphinidin-3-glucoside, delphinidin-3-rutinoside, petunidine-3-rutinoside. They were identified in all samples of anthocyanins. The proportion of the presence of one or another anthocyanidin in the studied samples depended on the method of isolation of anthocyanins. A more complete release of anthocyanidins is facilitated by the extraction of anthocyanins from blackcurrant berries at temperatures of 70°С for 30 minutes and 100°C for 5 minutes and extraction in an ultrasonic homogenizer at 25°C for 15 minutes. Under these conditions, all eight anthocyanidins are released. Based on this, a technology of the production of anthocyanin dye from berry raw materials was proposed. Developed colorant is a red liquid with a pronounced smell of black currant and a sour taste. Anthocyanin dye has an active acidity (pH) of 3.0, contains 2.99 mg/cm3 of anthocyanins in its composition and has antiradical activity.

Triticosecale "Bilinda" (cultivar was bred in the laboratories of the Leningrad Research Agriculture Institute, Branch of Russian Potato Research Centre, Leningrad Province) contains a large number of polyphenolic compounds which are biologically active components. The purpose of this work was to conduct a comparative metabolomic study of extracts of Triticosecale cultivar "Bilinda" seeds harvested at different stages of maturation. To identify target analytes in extracts HPLC was used in combination with an ion trap. The results showed the presence of 93 target compounds corresponding to Triticosecale. In addition to the reported polyphenol constituents, 28 polyphenols were newly annotated in grains of Triticosecale. There were 9 flavones, 7 flavonols, 2 flavan-3-ols, 1 flavanone, 5 hydroxybenzoic acids, 1 hydroxycinnamic acid, 1 anthocyanidin, 1 condensed tannin, 1 hydroxycoumarin, 2 triterpenoids; 1 diterpenoid, 6 sterols, 2 isoquinoline alkaloids, 4 carotenoids, 1 hydroquinone derivative and others bioactive compounds. Metabolic analysis showed a significant difference in the classes and amounts of polyphenolic substances identified in the seeds, depending on the time of seed collection.

: Actinidia deliciosa (A. Chev.) C.F. Liang & A.R& Ferguson, 1984 contains a large number of target analytes, which are biologically active compounds. HPLC-ion trap-MS/MS (tandem mass spectrometry) was used to identify target analytes in extracts of A. deliciosa (varieties Abbot, Alison, Bruno, Hayward, Monti), originating from the Adler’s Branch of N.I. Vavilov All-Russian Institute of Plant Genetic Resources. Separately, the pulp of the kiwi fruit and its peel were analyzed for the composition of biologically active substances. The richest in the composition of active substances was indicators of the cultivar Monti. The results of initial studies revealed the presence of 56 compounds in the extracts of the peel of A. deliciosa and the presence of 64 compounds in the extracts of berry pulp of A. deliciosa . Forty-three compounds were identified for the first time in family Actinidiaceae. New identified metabolites belonged to 17 classes including 13 flavones, 2 flavonols, 2 flavan-3-ols, 5 stilbenes, 4 lignans, 2 carotenoids, 2 sterols, 3 terpenoids, etc.

The purpose of this research is to investigate and identify polyphenolic complexes and other biologically active compounds by tandem mass spectrometry, presented in the leaves and stems of Ledum palustre L. Carbon dioxide, compressed to a supercritical state, was used for the most environmentally friendly extraction of polyphenolic complexes and other biologically active compounds of Ledum palustre L. The most effective extraction characteristics (pressure 350 bar; temperature 60 °С; extraction time 1-hour, co-solvent MeOH 3.5%) supercritical CO2-extraction of L. palustre were obtained empirically. To identify target analytes in supercritical extracts, High Performance Liquid Chromatography (HPLC) was used in combination with a BRUKER DALTONIKS ion trap. The results showed the presence of 61 biologically active compounds corresponding to the rhododendron species, of which 32 were identified for the first time in L. palustre. These are flavanols dihydrokaempferol, quercetin arabinoside, myricetin galactoside; flavones: diosmetin, nevadensin, cirsimaritin; flavanone naringenin; anthocyanins delphinidin, petunidin, cyanidin pentoside, delphinidin pentoside, peonidin 3-(6-O-acetyl) glucoside, peonidin-3-O-malonylglucoside, cyanidin-3-rutinoside, peonidin 3-O-glucoside; ellagic acid; lignan medioresinol; a type A procyanidin dimer; sterols fucosterol and avenasterol, etc.

The purpose of this work was a comparative metabolomic study of extracts of from Siberian breeds of the Solanum tuberosum L.: Tuleevsky, Kuznechanka, Memory of Antoshkina, Tomichka, Hybrid 15/F-2-13, Hybrid 22103-10, Hybrid 17-5/6-11, and Sinilga from the collection of Siberian Federal Scientific Centre of Agrobiotechnology of the Russian Academy of Sciences. HPLC was used in combination with ion trap to identify target analytes in extracts of tuber part of a potato. The results showed the presence of 87 target analytes corresponding to S. tuberosum. In addition to the reported metabolites, a number of metabolites were newly annotated in S. tuberosum. There were essential amino acid L-Tryptophan, L-glutamate, L-lysine, Nordenine; flavones Ampelopsin; Chrysoeriol, Diosmetin, Diosmin, Myricetin; flavanones Naringenin and Eriodictyol-7-O-glucoside; dihydrochalcone Phlorizin; oligomeric proanthocyanidin (Epi)afzelechin-(epi)afzelechin; Shikimic acid; Hydroxyphenyllactic acid; Fraxidin; Myristoleic acid; flavan-3-ols Epicatechin, Gallocatechin, Gibberellic acid, etc.

Aim. Search and selection of microorganisms with active enzymatic properties for possible biodestruction of pyrethroids. Materials and Methods. For effective screening and selection of the most active isolates, for the subsequent development of biotechnological methods for the destruction of pesticides and reduction of their toxicity, samples of the phylosphere and rhizosphere of agricultural crops, food products, etc. were taken. The isolates were evaluated by intracellular metabolism and the production of exoenzymes. The isolated microorganisms were identified on the basis of the "Bergi Bacteria Determinant". Screening of microorganisms for the development of biotechnological methods to reduce the toxicity of ecotoxicants included the following stages: selection of sources, sampling, seeding on a dense medium for isolation of pure culture, replanting of pure culture and investigation of biological properties of isolated strains. Results. In order to search for microorganisms capable of utilising pyrethroids, isolates of microscopic fungi and bacteria were obtained. Of the 23 selected strains, 12 had the widest spectrum of activity, while 5 strains showed the most pronounced and stable antagonistic activity against pathogenic microorganisms at various temperature parameters from 30°C to 42°C ( Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus ). For the selected strains, the environment was optimised to activate cellular metabolic processes. The activity of amylases, proteases, xylanases and cellulases of the fungus Trichoderma, and proteases of L. plantarum , L. lactis , B. subtilis and Propionibacterium was evaluated. Studies of the effect on the synthesis of hydrolytic enzymes of various concentrations in the culture medium in the presence of xylan polysaccharides, cellulose, starch and casein protein were carried out. Conclusion. Toxicological studies of the selected isolates and of a composition consisting of these isolates in the form of a culture suspension on the simplest stylonychia were carried out. Biotesting on isolated aquatic microorganisms ( Trichoderma , L. plantarum , L. lactis , B. subtilis and Propionibacterium) showed that the percentage of dead infusoria ( S. mytilus ) in both the experiment and the control showed no significant differences. A microbiological composition has been created that can be used to protect the environment when exposed to toxicants of agrotechnogenic origin. The selected strains were tested for the possibility of biodegradation of pyrethroids using the example of cypermethrin.

In this research, we present a detailed comparative analysis of the bioactive substances of soybean varieties k-11538 (Russia), k-11559 (Russia), k-569 (China), k-5367 (China), k-5373 (China), k-5586 (Sweden), and Primorskaya-86 (Russia) using an LSM 800 confocal laser microscope and an amaZon ion trap SL mass spectrometer. Laser microscopy made it possible to clarify in detail the spatial arrangement of the polyphenolic content of soybeans. Our results revealed that the phenolics of soybean are spatially located mainly in the seed coat and the outer layer of the cotyledon. High-performance liquid chromatography (HPLC) was used in combination with an amaZon SL BRUKER DALTONIKS ion trap (tandem mass spectrometry) to identify target analytes in soybean extracts. The results of initial studies revealed the presence of 63 compounds, and 45 of the target analytes were identified as polyphenolic compounds.

Drying is one of the ways to ensure the long-term preservation of fruits. The fruits quality depends on many factors, including drying conditions and their pomological variety. The study aim is to determine the drying parameter influence of the rosehip fruits of the pomological variety “Dessert” on the quality characteristics of the finished product. The study object is samples of dried rosehip fruits. A man dehydrated the freshly harvested fruits by air drying at temperatures of 50, 60 and 70 ° C, air flow rates of 0.5, 1.0 and 1.5 m/s–1 till reaching the 12 %-humidity. Research methods are common, standard. The researchers revealed that the organoleptic characteristics, the nutrient rosehip fruits composition, including biologically active substances, depend on temperature, air flow velocity and drying duration. The products, exposed to the dehydration at an air temperature of 50 °C, an air flow velocity of 1.5 m/s–1 for 25 hours, demonstrated the best quality characteristics. Dried rosehip fruits contain at least 6.0% dietary fiber, 780 mg /100 g of ascorbic acid, 250 mg/ 100 g of phenolic substances and 33.6 mg/100 g of beta-carotene. The shelf life of dried products is 24 months according as it is stored in corrugated boxes at a temperature not exceeding 20 °C and a relative humidity of not more than 75 %.