BACKGROUND:Accumulating evidences have stressed the association between hypoxanthine phosphoribosyl transferase 1 (HPRT1) overexpression and the poor prognosis of various cancers. Our study, herein, preliminarily investigates the involvement of HPRT1 in nasopharyngeal carcinoma (NPC). METHODS:Data from TCGA were applied to read HPRT1 expression in diverse cancers including NPC and to predict the prognosis of NPC patients. The total RNA and protein from NPC cells and nasopharyngeal epithelial cells NP460 were extracted to quantify HPRT1 expression. Following the completion of transfection, the proliferation and migration of NPC cells were determined employing MTT, colony formation and western blot assay (the quantification on expressions of protein related to proliferation and migration). RESULTS:HPRT1 was differentially expressed in diverse cancers yet particularly highly expressed in NPC, and high HPRT1 expression was related to the poor prognosis of NPC patients. Also, HPRT1 expression was higher in NPC cells and its silencing diminished the viability and proliferation of NPC cells and reduced the expressions of CyclinD1, CyclinE, Multidrug Resistance Protein 1 (MDR1), matrix metalloproteinase (MMP)-2, and MMP-9. CONCLUSION:This study preliminarily explored the involvement of HPRT1 in NPC based on some cellular assays in vitro, which may provide evidence for investigating the specific mechanism underlying the effects of HPRT1 in cancers.

目的探讨白介素33（IL-37）、白介素37（IL-37）在慢性鼻窦炎（chronic rhinosinusitis,CRS）患者中的表达。方法收集23例鼻息肉（chronic rhinosinusitis with nasal polyps,CRSw NP）患者的鼻息肉黏膜和10例慢性鼻窦炎（chronic rhinosinusitis without nasal polyps,CRSs NP）患者的窦口鼻道复合体黏膜作为实验组; 12例鼻中隔偏曲合并慢性鼻炎患者下鼻甲粘膜作为对照组。采用实时荧光定量反转录聚合酶链反应（RT-PCR）分别检测各组中IL-33、IL-37mRNA的相对表达量。结果 IL-33 mRNA在CRSw NP组、CRSs NP组和对照组均有表达,其值分别为4. 326±1. 910,3. 565±1. 746,2. 737±1. 985,差异无统计学意义（F=2. 818,P=0. 071）。IL-37 mRNA在CRSw NP组、CRSs NP组和对照组均有表达,其值分别为6. 826±2. 235,5. 019±1. 671,3. 133±1. 678,差异有统计学意义（F=13. 888,P=0. 000）。随着IL-33水平增加,慢性鼻窦炎患者IL-37 mRNA水平也增加,并有相关性（r2=0. 159,P=0. 007）。结论 IL-33、IL-37在CRS鼻粘膜中异常表达可能在CRS的发生、发展的过程中发挥一定作用。

目的 探讨硬性耳内镜下鼓室置管术、鼓膜切开术及穿刺术治疗分泌性中耳炎的临床疗效及安全性.方法 90例保守治疗无效的分泌性中耳炎患者随机分3组,置管组采用耳内镜下鼓室置管术,切开组采用鼓膜切开术,穿刺组采用鼓膜穿刺术治疗,观察对比三种治疗方法的治疗效果、并发症及复发情况.结果 三组患者的临床总有效率及并发症情况均有统计学意义,置管组的临床有效率明显高于后两组,并发症发生率明显低于后两组.结论 硬性耳内镜下鼓室置管手术,视野宽、清晰,立体感强,手术操作精细、创伤小,疗效可靠,并发症及复发率低,可同期处理腺样体疾病,值得临床推广应用.

OBJECTIVE:This paper is aimed to analyse the pathogeny of postnasal drip syndrome including of cough, postnasal drip and pharyngeal foreign body sensation. And explore to the appropriate diagnosis and treatment strategy.METHOD:The author took 56 cases of PNDs as examples. The 56 cases were switched to FESS. The author frequently visit the 56 patients after treatment for 6 months and did SNOT-20 (the sino-nasal outcome test-20) scale assessment of the symptom improvement in patients before and after the situation. The author also compared the symptom score differences between postnasal drip and cough and explored the disease causes and assess the postoperative recovery status.RESULT:In patients with 56 cases PNDs, chronic rhinosinusitis in 22 patients (39.3%), nasal polyps in 11 patients (19.6%), deviation of nasal septum partial in 5 patients (8.9%), the adenoids in 10 patients (17.9%), the fungal sinusitis in 5 patients (8.9%), the maxillary sinus hemorrhagic polyp in 2 cases (3.6%), the nasal-sinal neoplasm in 1 cases (1.8%). SNOT-20 rating scale result showed:r = 0.674 (P < 0.01), r = 0.062 4 (P < 0.01), postnasal drip and cough symptoms score with certain relevance. Before and after the operation of score comparison about the "postnasal drip" symptoms, scoring significant difference (P < 0.01), namely the surgical treatment had distinct effect. And "cough" symptoms score before and after operation had a significant difference (P < 0.01).CONCLUSION:"postnasal drip" and "cough" symptoms occur with some relevance. After the FESS the two symptoms are improved significantly.

Objective To evaluate the surgical effect for nasal diseases-combined asthma,and to discuss the significance of nasal function examination in the diagnosis and treatment.Methods According to the combined diseases,the asthma patients were divided into nasal septum group,nasal polyps group,and sinusitis group.The surgical effect,improvement of nasal ventilation function and relief of asthma were evaluated and compared across groups via nasal function examination combined with the improved asthma control questionnaire(ACQ6).Results The nasal function examination showed significant postoperative changes in all groups.The differences of nasal function change between the nasal septum group and nasal polyps group,sinusitis group were statistically significant(P 0.05),while that between the nasal polyps group and the sinusitis group was insignificant(P 0.05).Statistical results of ACQ6 showed significant differences between the scores before and after operation in all groups,which indicated that asthma symptoms got relieved after operation(P 0.05).And the group comparison results are not statistically significant(P 0.05).Conclusions Symptoms of asthma can be relieved significantly via surgical treatment of accompanied nasal diseases.Combined application of nasal function examination and ACQ6 may provide a new strategy for the diagnosis and treatment of nasal diseases-combined asthma.

OBJECTIVETo explore the changes of nasal airway resistance (NAR) and olfactory recovery in patients with olfactory disorder before and after endoscopic sinus surgery.METHODOlfactory functions and NAR in 105 patients with olfactory disorder diagnosed as chronic rhino-sinusitis and/or nasal polyps were measured with five taste test olfactory liquid and anterior rhinomanometry. The patients were grouped according to their olfactory disturbance degree before the surgery and recovery of olfaction after the surgery.RESULTBefore the surgery, the degree of olfactory disorder were accompanied with varied clinic classifications. Six months after the surgery, the patients whose conditions were less serious before surgery recovered better. Before and after the surgery, significant difference of the nasal airway resistance was found between the three-groups whose olfactory disorder degree were different. After the surgery, no difference was found of the nasal airway resistance between the three-groups whose olfactory disorder degree were different before the surgery and the recovery of olfaction degree were different after the surgery.CONCLUSIONEndoscopic sinus surgery can decrease the nasal airway resistance of patients with olfactory disorder. We can evaluate the efficacy of endoscopic sinus surgery more objectively combined with nasal airway resistance analysis.

Objective To investigate the method and curative effect of three stepwise treatment for pediatric patients with chronic rhinosinusitis(CRS).Methods The clinical data of 100 children with chronic sinusitis were retrospectively analyzed.Firstly,expectant treatment systemically; Secondly,auxiliary surgery;Thirdly,endoscopic sinus surgery.Results All patients were followed up for 12 - 18months.Forty-two cases were treated systemically with medicine,30 cases (71％) were cured,9 cases (21％) improved and 3 cases(8％ ) showed no effect.Thirty-five cases were treated with auxiliary surgery,28 cases(80％ ) were cured,5 cases( 14％ ) improved and 2 cases(6％ ) showed no effect.Twenty-three cases were treated with endoscopic sinus surgery,17 cases(74％ ) were cured,4 cases( 17％ )improved and 2 cases (9％) showed no effect.The overall efficiency was 93％.Conclusions Three stepwise treatment for chronic rhinosinusitis is effective in children.