Objectives:Gestational diabetes mellitus (GDM), one of the most common metabolic disorders in pregnancy, impacts maternal and fetal health. This study was designed to assess the effects of mild GDM on the histology, ultrastructure, and morphometry of fetal liver tissue. Materials and Methods:In this experimental study, twenty pregnant rats were randomly allocated into control and streptozotocin (STZ)-induced diabetic groups. Mild hyperglycemia was induced by intraperitoneal injection of STZ (40 mg/kg/bw) on the 5th day of gestation. At day 19 of gestation, fetal livers were separated and subjected to histological, transmission electron microscopic, and quantitative morphometric examinations. Results:In the GDM group, PAS staining was positive, revealing scattered eosinophilic inclusions in some hepatocytes. Masson trichrome staining was also positive and showed some fibrous tissue as fine fibers in the portal spaces that extended to the central vein. Reticulin staining in the GDM group was focally positive in the areas of fibrosis and the portal spaces. Ultrastructural examination showed pyknotic nuclei, karyolysis, degranulation and vesiculation of the rough endoplasmic reticulum, and degeneration of mitochondria in the GDM group. The morphometric examination demonstrated that the mean area of hepatocytes was significantly lower in the GDM group than in the control group (P<0.05). Moreover, the mean diameter of the central vein and the density of megakaryocytes were significantly higher in the GDM group than in the control group (P<0.05). Conclusion:Uncontrolled mild GDM induced the histological, ultrastructural and morphometric alterations in the fetal liver.

Background: Gestational diabetes mellitus (GDM) is one of the most common pregnancy complications that can affect the organ systems of the body in both the mother and fetus. This study aimed to determine the impact of mild GDM on maternal and fetal liver histopathological alterations. Materials and Methods: In this experimental study, 20 pregnant Wistar rats were randomly allocated in control and diabetic groups. Mild hyperglycemia was induced by intraperitoneal injection of streptozotocin (40 mg/kg body weight) on the 5th day of gestation. The control group received an equal volume of citrate buffer. The diabetic state was confirmed by a blood glucose level of 120-300 mg/dL. Maternal and fetal liver samples were obtained on day 19 of gestation and stained with hematoxylin and eosin for histopathological investigation. Results: Liver sections of diabetic dams exhibited edematous hepatocytes and scattered pyknotic and necrotic cells with dilated sinusoids and congested central veins. The portal tracts showed the proliferation of bile ducts with mild chronic inflammatory cells infiltrating together with fibrosis beyond the limited plate which extends to the central vein (porto-central fibrosis). Liver sections of their fetuses revealed edematous hepatocytes with increased necrotic cells, with pyknotic nuclei, dilatation of the hepatic sinusoids, and their central veins. There was also a relative increase in megakaryocytes, which promoted fibrosis and distorted vascular beds of the hepatic tissue. The portal tracts also showed bile duct proliferation. Conclusion: This study highlighted the adverse effects of uncontrolled mild GDM on liver structure in rat dams and their fetuses.

BACKGROUND: Diarrhea in patients on enteral feeding is a common complication with serious clinical outcomes. In Eastern and Southern Mediterranean regions, using curd as a food for the treatment of diarrhea is a common practice. OBJECTIVE: This study aimed to investigate whether curd could reduce the duration or severity of diarrhea in critically ill tube-fed patients with head trauma. METHODS: A total of 42 patients on enteral feeding who had diarrhea after starting enteral feeding, admitted to the intensive care unit, were randomly assigned to either to receiving curd and standard enteral formula or standard enteral formula only groups. The intervention period for each patient was three days. The primary outcome was the duration of diarrhea, while the secondary outcomes were the frequency and weight of stool per day in each patient. RESULTS: This study showed a significantly higher stool weight in patients receiving enteral curd (588.76±266.88 vs 390.60±171.82 gr/day). There was no significant difference between the groups in duration of diarrhea (9 v 8 days) and episodes of diarrhea per day (2.20±1.00 vs 2.00±0.70) in intervention and control groups. incidence of liquid or loose stools on days 1–4 of the intervention (P1 = 0.43, P2 = 0.16, P3 = 0.45, P4 = 0.62). Feeding with curd did not change the APACHE score in intervention and control groups. This study showed no significant difference in 28 days of mortality in intervention and control groups. CONCLUSION: The findings showed that supplementary enteral feeding with curd did not improve diarrhea, the severity of the disease, or 28 days mortality in critically ill patients with diarrhea receiving enteral nutrition.

Spinal cord injury (SCI) as a crippling disability causes tissue degeneration via neuron loss and fiber disruption. Some researchers have tried to reverse or minimize these changes. Platelet-rich plasma (PRP) is a biological product derived from peripheral blood containing a variety of growth factors. PRP has been extensively used in regenerative medicine. On the other hand, via secreting neuroprotective growth factors, mesenchymal stem cells (MSCs) have shown a promising potential in repairing central nervous system deficits. This study investigated the therapeutic effect of the combined use of MSCs and PRP in a rat model of SCI. We used real time-PCR method for evaluation of Bcl-2, Bax and caspase 3 expressions, TUNEL test for apoptotic cell death assessment, and neurofilament NF200 immunohistochemistry for examination of axonal regeneration. The results showed that co-treatment with MSCs and PRP efficiently alleviated the evaluated categories. Significant differences were observed in expression of Bcl-2 and caspase3, but not Bax, apoptotic index and the number of NF200 positive axons (for all P ≤ 0.01) between co-treatment animals compared with those treated with only MSCs or PRP. In conclusion, this study showed that combination of MSCs and PRP synergistically promotes their therapeutic effects in the SCI.

Background and Objectives: In recent years, reports of Acinetobacter strains resistant to all known antibiotics have caused a great concern in medical communities. Overexpression of efflux pumps is one of the major causes of resistance in bacteria. The aim of this study was to investigate the role of efflux pumps in conferring resistance to imipenem in clinically important Acinetobacter spp; Acinetobacter baumannii and Acinetobacter lwoffii. Materials and Methods: A total number of 46 clinical Acinetobacter isolates, including 33 A. baumannii and 13 A. lwoffii isolates, previously collected from Shahid Kamyab and Ghaem hospitals of Mashhad, Iran were used in this study. Imipenem susceptibility testing was carried out by the disc diffusion method. Imipenem minimum inhibitory concentration (MIC) for resistant Acinetobacter isolates were determined both in the presence and absence of the efflux pumps inhibitor, carbonyl cyanide 3-chlorophenylhydrazone (CCCP). Results: Resistance to imipenem was observed in 38 isolates including 30 A. baumannii and 8 A. lwoffii isolates. Experiments in the presence of CCCP showed a 2 to 16384 fold reduction in imipenem MICs in 14 A. baumannii and 2 A. lwoffii isolates. Conclusion: The results obtained showed high levels of resistance to imipenem and contribution of efflux pumps in conferring resistance in both Acinetobacter species in this study. Moreover, imipenem efflux mediated resistance highlights the importance of this mechanism not only in A. baumannii but also in non-baumannii Acinetobacter Spp. which have been neglected in antibiotic resistance studies.

Using cell-based engineered skin is an emerging strategy for treating difficult-to-heal wounds. To date, much endeavor has been devoted to the fabrication of appropriate scaffolds with suitable biomechanical properties to support cell viability and growth in the microenvironment of a wound. The aim of this research was to assess the impact of adipose tissue-derived mesenchymal stem cells (AD-MSCs) and keratinocytes on gelatin/chitosan/β-glycerol phosphate (GCGP) nanoscaffold in full-thickness excisional skin wound healing of rats. For this purpose, AD-MSCs and keratinocytes were isolated from rats and GCGP nanoscaffolds were electrospun. Through an in vivo study, the percentage of wound closure was assessed on days 7, 14, and 21 after wound induction. Samples were taken from the wound sites in order to evaluate the density of collagen fibers and vessels at 7 and 14 days. Moreover, sampling was done on days 7 and 14 from wound sites to assess the density of collagen fibers and vessels. The wound closure rate was significantly increased in the keratinocytes-AD-MSCs-scaffold (KMS) group compared with other groups. The expressions of vascular endothelial growth factor, collagen type 1, and CD34 were also significantly higher in the KMS group compared with the other groups. These results suggest that the combination of AD-MSCs and keratinocytes seeded onto GCGP nanoscaffold provides a promising treatment for wound healing.

Background: The development of multidrug-resistant Acinetobacter species has created serious problems in nosocomial infections. Understanding the underlying resistance mechanisms and their significance in conferring resistance to different antibiotics is the first step to develop strategies for fighting or reversing the current resistance. Objectives: The aim of this study was to investigate the role of efflux pumps in decreasing susceptibility to amikacin in Acinetobacter clinical isolates. Methods: Forty-six clinical Acinetobacter isolates were collected from 2 teaching hospitals of Mashhad, Iran. Susceptibility testing was conducted by the disc diffusion method. Amikacin minimum inhibitory concentration (MIC) for resistant Acinetobacter isolates was determined according to the Clinical and Laboratory Standards Institute (CLSI) guidelines either with or without the efflux pumps inhibitor, carbonyl cyanide 3-chlorophenylhydrazone (CCCP). Conventional polymerase chain reaction (PCR) was used to analyze the presence of pump genes. Results: Acinetobacter isolates were identified as 2 species; Acinetobacter baumannii and A. lwoffii. Susceptibility testing showed high levels of resistance to amikacin in 27 isolates, including both A. baumannii and A. lwoffii, among which 20 A. baumannii isolates showed a 2- to 524288-fold reduction in amikacin MIC in the presence of CCCP, while no reduction occurred in amikacin MIC in resistant A. lwoffii isolates. The PCR results showed high frequencies of adeB, abeM, and adeI genes in Acinetobacter isolates yet the adeE gene was not found in any of the isolates. Conclusions: The obtained results indicated the importance of efflux pumps in conferring resistance to amikacin in clinical isolates of A. baumannii, yet not in A. lwoffii.

ObjectiveThe aim of the present study was to evaluate the effect of PRP on the repair of spinal cord injury in rat model.Material and methodsRats were randomly divided into three groups with six rats in each group. Then, spinal cord injury was performed under general anesthesia using “weight dropping” method. Control group included rats receiving normal saline, group two received PRP 1 week after injury; group three received PRP 24 h after injury. The motor function was assessed weekly using the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale. Anterograde tracing was performed for evaluation of axon regeneration.ResultMotor recovery was significantly better in the rats treated with PRP 24 h after injury than the control group. In the rats treated with PRP 1 week after injury and rats treated with PRP 24 h after injury, the average numbers of BDA-labeled axons were statistically different from the control group.ConclusionOur experimental study demonstrated positive effects of platelet rich plasma on nerve regeneration after spinal cord injury.

Scaffold-based tissue engineering is considered as a promising approach in the regenerative medicine. Graft instability of collagen, by causing poor mechanical properties and rapid degradation, and their hard handling remains major challenges to be addressed. In this research, a composite structured nano-/microfibrous scaffold, made from a mixture of chitosan-ß-glycerol phosphate-gelatin (chitosan-GP-gelatin) using a standard electrospinning set-up was developed. Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions were prepared at ratios of 30/70, 50/50, 70/30 (w/w) and their mechanical and biological properties were engineered. Furthermore, the pore structure of the fabricated nanofibrous scaffolds was investigated and predicted using a theoretical model. Higher gelatin concentrations in the polymer blend resulted in significant increase in mean pore size and its distribution. Interaction between the scaffold and the contained cells was also monitored and compared in the test and control groups. Scaffolds with higher chitosan concentrations showed higher rate of cell attachment with better proliferation property, compared with gelatin-only scaffolds. The fabricated scaffolds, unlike many other natural polymers, also exhibit non-toxic and biodegradable properties in the grafted tissues. In conclusion, the data clearly showed that the fabricated biomaterial is a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices. These results suggested the potential of using mesoporous composite chitosan-GP-gelatin fibrous scaffolds for engineering three-dimensional tissues with different inherent cell characteristics.