Abstract Green synthesis of metallic nanoparticles using plant extracts has emerged as an environmentally friendly and sustainable alternative to conventional chemical and physical fabrication methods. In the present study, silver nanoparticles (AgNPs) were biosynthesized using aqueous extracts of Achillea arabica leaves and flowers, which served as natural reducing and stabilizing agents. The formation of nanoparticles was initially confirmed by a rapid color change of the reaction mixture and further characterized using UV–visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). UV–Vis spectra revealed characteristic surface plasmon resonance peaks at 450 nm for leaf-derived nanoparticles (AgNPsLe) and 490 nm for flower-derived nanoparticles (AgNPsFl), confirming successful nanoparticle formation. XRD analysis indicated the crystalline nature of the nanoparticles with a face-centered cubic (fcc) silver structure, with crystallite sizes ranging from 13–20 nm. SEM micrographs revealed distinct morphological differences, where AgNPsLe predominantly exhibited cubic structures, while AgNPsFl were mainly spherical, suggesting that phytochemical composition influences nanoparticle nucleation and growth orientation. The biological activities of the extracts and synthesized nanoparticles were assessed through antioxidant, antibacterial, and antibiofilm assays. The flower extract showed the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (81.68%), whereas AgNPsLe demonstrated the strongest Ferric Reducing Antioxidant Power (FRAP) activity with a Half maximal inhibitory concentration (IC50) of 0.139 mg mL− 1. Antibacterial evaluation revealed that AgNPsFl exhibited the highest antimicrobial potency with Minimum Inhibitory Concentration (MIC) values ranging from 37.5–75 μg mL− 1, while AgNPsLe showed MIC values between 75 and 150 μg mL⁻1. Furthermore, the synthesized nanoparticles significantly inhibited bacterial biofilm formation with inhibition rates reaching approximately 64%.These findings highlight the potential of A. arabica-mediated AgNPs as promising bioactive nanomaterials for antimicrobial and biomedical applications, emphasizing the role of plant-derived phytochemicals in controlling nanoparticle morphology and biological activity.

Objectives: This study examined the impact of various abiotic factors, specifically temperature and precipitation, on the yield and composition (volatile profile) of essential oils (EO) from four Eucalyptus species, including Eucalyptus salmonophloia , Eucalyptus torquata, Eucalyptus lesouefii and Eucalyptus astringens . Additionally, the antimicrobial properties of these EO were assessed. Methods: The species were collected from five arboreta in Tunisia belonging to two climatic conditions (arid and semi-arid). EOs were extracted from the leaves using the hydrodistillation technique and analyzed by gas chromatography coupled with mass spectrometry (GC-MS). The antimicrobial activity was evaluated by measuring the diameters of inhibition zones using the agar well diffusion method and by determining the minimum inhibitory concentrations (MICs). Results: The yield of Eucalyptus EOs varied from 0.12% to 4.63% (w/w, dry weight) depending on the species and the plant's growing location. 1,8-cineole (29.71% to 67.16%) was by far the major compounds in EOs of E. salmonophloia, E. lesouefii and E. astringens , however E. torquata was torquatone chemotype (33.41% to 44.78%). In general, the aridity increased the extraction yield of EO. Higher temperature and lower rainfall conditions enhanced the production of key compounds such as, 1,8-cineole and ɑ-pinene, however, it decreases others compounds like spathulenol and viridiflorol. A notable antimicrobial activity was observed against all microbial strains tested, demonstrating both microbicidal and microbiostatic effects, particularly against Escherichia coli , Serratia marcescens , and Candida tropicalis . The EOs derived from the studied Eucalyptus species represent a valuable source of bioactive compounds, including 1,8-cineole, ɑ-pinene, spathulenol, and β-eudesmol. These compounds contribute to the oils’ significant antimicrobial efficacy, offering the additional advantage of being a natural product. Conclusion: Our findings reinforce the notion that environmental factors may serve as a limiting factor in the production and availability of Eucalyptus EO for medicinal and industrial applications.

Herein, we describe the synthesis, structural characterization and functional properties of a new cadmium(II) complex which was investigated by single-crystal X-ray diffraction, UV-Visible spectroscopy, luminescence studies, dielectric property, antimicrobial activities and molecular docking simulations. A mononuclear structure was obtained, with an octahedral geometry of cadmium determined with X-ray diffraction. Spectroscopic characterization of the complex revealed its functional and electronic properties. The luminescence measurements showed interesting features of its photophysics. The dielectric properties including the dielectric constant (epsilon '), dielectric loss (epsilon ''), and electrical conductivity (sigma) were examined as functions of both temperature and frequency. To gain insight into the electrical conduction mechanism, impedance analysis was performed using Cole-Cole plots. The complex exhibited significant antimicrobial activity, outperforming standard reference compounds determined by the agar well diffusion method. In addition, molecular docking approach was used in order to study the binding affinities and molecular interactions formed between the docked cadmium(II) complex and the target receptors. The docking outcomes highlighted a strong affinity for biological targets, suggesting potential applications in bio-imaging and as antimicrobial agents.

Pinus halepensis is an excellent medicinal plant that can remedy many health problems. The present study aimed to investigate antioxidants in two seasons (spring and summer) and the antimicrobial and antifungal activities of ethanolic extracts (EEs) of three provenances: Affra and Oum Jedour (Tunisian provenances), and Guargano (Italian provenance). In August, the ethanolic extract of Guargano provenance was the most important and highest source of phenolic compounds (P < 0.05). Likewise, this EE exhibited the most significant antioxidant activity values in both seasons at P < 0.05. Similarly, the antibacterial potential of these EE was evaluated against human clinical pathogenic bacteria and selected Candida strains. EEs suppressed bacterial proliferation by creating concentration-dependent spheres of inhibition. Thus, the Guargano provenance (March and August) was more potent against all tested bacterial strains and Candida sake. However, the Oum Jedour ethanolic extract was the most effective against Candida albicans. Moreover, Affra extract reduced blue rot disease in Citrus fruit by 60.58

In this work, polymeric copper complex [Cu(NO3)2(C12H8N2)]n, where C12H8N2= phenantroline (phen) has been prepared using slow evaporation method and characterized through single-crystal X-ray diffraction, photoluminescence, IR and UV/DRS diffuse reflectance spectroscopy from which the optical proprieties were determined. X-Ray crystal analysis of the complex confirmed that the copper ion displays a distorted square-pyramidal environment. The cohesion and stabilization of the structure are provided by the π-π interaction among the aromatic rings of the phen. The optical spectra indicate that the compound has a direct band gap (1.14 eV). The intermolecular interactions were examined using Hirshfeld surfaces by investigating the derived 2D fingerprint plots and enrichment ratios. Antimicrobial potential, anti-biofilm and anti-virulence effect were also carried out to evaluate the biological activities of the complex.

Over the past few decades, silver nanoparticles gained much attention regarding their unique properties and have proven various applications in medicine, catalysis, and biotechnology. AgNPs are well-known as antimicrobial agents and have significant inhibitory action against microbial pathogens. Several scientific research interests in the knowledge of the mechanisms of silver nanoparticles and their influence on living pathogens. Due to the massive discussion of the mechanism actions against bacterial cells, here we describe those against fungal cells and viruses which were not well studied. This chapter investigates the various approaches used to synthetize the silver nanoparticles, and the potential use of the green silver nanoproducts. The application and mechanism actions of AgNPs were also described. The nontoxicity, and multiple potential applications of silver bio-nanoparticles make them as a promising alternative as antimicrobial agents, anti-inflammatory molecules, wound healing, antiviral, anticancer, and anti-Alzheimer's disease. Finally, future applications of silver nanoparticles have been presented.

The study aimed to evaluate the biosurfactants (BSs) production by SM-23 strain of Virgibacillus identified by phenotypical and WGS analysis as Virgibacillus massiliensis. We first demonstrated the lipopeptides production by Virgibacillus massiliensis specie and studied their biochemical and molecular analysis as well as their biological potential. The GC–MS analysis indicated that methyl.2-hyroxydodecanoate was the major fatty acid compound with 33.22

The new bis(1-4(chlorophenyl)piperazinium) tetraisothiocyanatecobaltate(II) crystal was prepared by slow evaporation method, and structurally characterized by single crystal X-ray diffraction analysis. The anhydrous compound (C10H14N2Cl)2 [Co(NCS)4] crystallized in the space group (P1, Z = 2), its structure consisted of NH2+ groups and inorganic anions, which are linked via N-H...S hydrogen bonds to form anionic layer's parallel to (a, b) plane. The organic cations are trapped between anionic layers to form a three-dimensional network structure. The inter-contacts in the crystal packing were envisioned quantitatively using Hirshfeld surface method by 2D fingerprint plots. The vibrational properties were discussed experimentally by means of IR spectroscopy. Electronic and computational features are examined by quantum chemical studies. The antimicrobial effects were surveyed in vitro against clinical bacterial strains. Molecular docking investigation divulged significant interactions and binding affinities into active sites. From these results, the Co(II) complex displayed considerable biological activity against Human Immunodeficiency Virus (HIV) II Protease.

We report herein the synthesis and physicochemical characterization of a new 8-hydroxyquinoline cobalt(III) complex ethanol solvate with formula [Co(C9H6NO)3].(C2H6O). This compound was been prepared by slow evaporation at room temperature and characterized by single crystal X-ray diffraction. It crystallizes in the monoclinic system, spaces group P21/n. The central cobalt ion has a slightly distorted square bipyramidal environment, coordinated by three chelating 8-hydroxyquinoline molecules. Structural cohesion is primarily established by 7C-7C interactions between the neighboring rings of quinoline groups and intermolecular hydrogen bonds O-H...O connecting the cobalt complex entities and uncoordinated ethanol molecules. The title compound has been characterized by the infrared (IR) and the UV-Visible (UV-Vis) spectra. Intermolecular interactions in the crystal structure were quantified by Hirshfeld surface analysis. The band gap of the complex was calculated using solid-state reflectance spectra, and the results show that the complex acts as a semiconductor. Moreover, the new compound exhibits high antibacterial and antifungal activities, as detected by the well agar diffusion against various bacterial and fungal clinical strains. The superior inhibitory effect was observed against Klebsiella pneumoniae, Candida albicans and Candida glabrata with zones inhibition of about 17.5, 17.5 and 17 mm, respectively.

A new cobalt (II) complex was synthesized and characterized by physicochemical evidences. According to crystallography study, the molecular structure consisting of anionic hybrid layer, while [C6H17N3]2+ cations, are mainly trapped onto successive between these latter, forming corrugated organic layers. This analysis suggests a distorted tetrahedral geometry around cobalt (II) having a CoN4 coordinating atmosphere. The crystal packing is stabilized by intra-and intermolecular hydrogen bonds. The vibrational properties were discussed by infrared spectroscopy to identify functional groups. Hirshfeld surface analysis and corresponding 2D fingerprint plots have been performed in order to elucidate short contacts resulting from the structural data. The new compound gave strong antimicrobial potential against the most treated microbial pathogens, with lysozyme and anti-biofilm activities. Molecular docking interactions between the complex and Salmonella enterica protein 4K6L forming conventional hydrogen bonds with amino acid residues LYS E:83 G:128, ARG C:90 and ASP E:87 with bond lengths of 2.35, 2.40 and 3.53 angstrom, respectively, and a binding affinity of-4.2 Kcal/mol. The complex [Co(NCS)4]2 (C6H17N3)2. 4H2O bonded with the protein 7EE6 with binding affinity of about-4.3 Kcal/mol forming con-ventional hydrogen bonds with amino acid residues LYS A:90, ASN B:90, ETH E:88, LYS G:242, ASN B:87 and GLU G:23 with bond lengths of 2.68, 2.82, 241, 2.65, 3.59 and 3.78 angstrom, respectively. From these results, despite not matching the docking performance of doxycycline and ciprofloxacin, the compound displayed considerable biological feasibility. Therefore, the study leaves space for experimental studies.

Heavy metal pollution is a major environmental issue that has a negative impact on soil quality and food security. As a result, heavy metal removal or remediation from hazardous sites has become mandatory. Bioremediation based on microorganisms is a promising method to remediate heavy metal-contaminated areas due to its eco-friendly, cost-effective, and highly efficient characteristics. This study aimed to isolate, identify and characterize rhizospheric bacteria able to resist, reduce, and detoxify heavy metals (chromium [Cr], nickel [Ni], and aluminum [Al]) from agricultural soil. Two isolates were chosen due to their high level of heavy metal resistance and could serve as a potential in situ remediation agent at the site of isolation. On the basis of morphological, cultural, biochemical, and molecular characterization, these two isolates were identified as Pseudomonas aeruginosa (S1) and Bacillus cereus (S2). Results revealed a Minimum Inhibitory Concentration (MIC) of the three heavy metals studied ranging from 1000 µg/ml to 1400 µg/ml for the two bacterial isolates. The atomic absorption spectroscopy analysis was used to evaluate the degrading potential. Bacillus cereus was able to reduce Cr and Al more than Pseudomonas aeruginosa (42 and 67.78% vs 38.44 and 58.85, respectively). On the other hand, Pseudomonas aeruginosa showed a higher capacity to degrade Ni than Bacillus cereus (62.33% and 50.76%, respectively). The findings of the analysis deliver information for these heavy metals resistant bacterial isolates to become a potential bioremediation agent in contaminated environment.

This study focuses on the phytochemical analysis of the leaves of three Mediterranean species from natural community native to Tunisia. A separated and a mixed leaves extracts were prepared using three species (Ceratonia siliqua L. (C), Pistacia lentiscus L. (L) and Olea europaea L. var. sylvestris or oleaster (O)). Four leaves hydroethanolic extracts including C, L, O and mixed extract coded MCLO (1/3, 1/3, 1/3) were used. The total polyphenols, flavonoids and condensed tannins were determined by colorimetric method. The identification of phenolic profile was performed HPLC-DAD/MS analysis. The antioxidant activity was evaluated by two tests and the antimicrobial activity was done against some clinical microbial pathogens. Results showed that the mixture MCLO extract gave the highest free radical-scavenging activity (IC50 = 0.057 g/mL) and showed an amount of 35.347 mg GAE /g DM of total polyphenols content. As well, the MCLO exhibited significant antibacterial and antifungal activities against the tested pathogen strains except of Candida albicans. Likewise, the MCLO possesses lysozyme activity against Staphylococcus aureus (282.66 AU/mL/min) and showed the highest percentage of inhibition on biofilm formation (97.28%). A total of 14, 11 and 8 phenolic compounds were identified in C, Land O extracts, respectively. Though, in the mixture MCLO extract, 21 phenolic compounds were identified. The high biological activities found with the mixture extract could be related to the three distinguished phenolics acids; trigalloyl-glc (261.10 mg/mL), trigalloylquinic acid (254.68 mg/mL), and digalloyl-glc (225.21 mg/mL). These finding highlights the improvement of the biological activities of carob, lentisk and oleaster using mixed leaves extracts. (c) 2024 SAAB. Published by Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.

Nanoparticle synthesis for biological devices is one of the most promising research fields invading the world. This work aimed to synthesize ecofriendly silver nanoparticles (AgNPs) with enhanced biological activities. A green method was adopted to synthesize, for the first time, AgNPs from Fraxinus angustifolia extracts. The AgNPs structural and morphological properties were characterized. Their antioxidant, antimicrobial and antidiabetic effects were investigated. Their cytotoxic and genotoxic effects were explored using the novel method of acetocarmine dye. Results confirmed the formation of stable, mono-dispersed and spheric nanoparticles with a Face-Centered Cubic crystalline structure, sized from 30.12 to 49.62 nm. The AgNPs showed high antioxidant activities (DPPH, β-carotene bleaching and total antioxidant activities). They significantly inhibited the growth of the multi-drug resistant pathogens Escherchia coli and Candida tropicalis. However, they showed moderate inhibitory activity against biofilm production of E. coli and C. tropicalis, with 65 and 63% inhibition, respectively. F. angustifolia AgNPs exhibited interesting antidiabetic activity, exceeding that of the acarbose. Interestingly, the acetocarmine stanning was approved as an exploration technique for the screening of cytotoxic and genotoxic effects. Allium cepa epidermis tissues treated by the AgNPs presented a visible morphological change in the cell membrane and the disruption of the nuclear envelope. The obtained results highlighted the versatile biological potential of F. angustifolia AgNPs allowing their use as green nanomaterials in food preservation, pharmaceutical formulation and in food quality prospecting. This work also affords a new method for screening possible cytotoxicity and genotoxicity of nanomaterials in agri-food applications.

We report herein the synthesis and the physicochemical characterization of a new 8-hydroxyquinoline cobalt(III) complex ethanol solvate of formula [Co(C9H6NO)3].(C2H6O). This compound has been prepared by slow evaporation at room temperature and characterized by single crystal X-ray diffraction. This compound crystallizes in the monoclinic system, spaces group P21/n. The central cobalt ion has a slightly distorted square bipyramidal environment, coordinated by three chelating 8-hydroxyquinoline. Structural cohesion is established essentially by π-π interactions between the neighbouring rings of quinoline groups and intermolecular hydrogen bonds O-H…O connecting the cobalt complex entities and uncoordinated ethanol molecules.The title compound has been characterized by IR and UV-VIS spectra. Intermolecular interactions in the crystal structure were quantified by Hirshfeld surface analysis. The band gap of the complex has been calculated using solid state reflectance spectra, and results show that complex act as semiconductor. Moreover, the new compound exhibits high antibacterial and antifungal activities detected by the well agar diffusion against various bacterial and fungal clinical strains. The superior inhibitory effect was given against Klebsiellapneumoniae,Candida albicans and Candida glabratawith zoneinhibition of about 17.5, 17.5 and 17 mm, respectively.

Novel silver nanoparticles were synthesized based on a simple and non-toxic method by applying the green synthesis technique, using, for the first time, the aqueous extract of an extremophile plant belonging to the Achillea maritima subsp. maritima species. AgNP characterization was performed via UV-Visible, front-face fluorescence spectroscopy, and FTIR and XRD analyses. AgNP formation was immediately confirmed by a color change from yellow to brown and by a surface plasmon resonance peak using UV-Vis spectroscopy at 420 nm. The biosynthesized AgNPs were spherical in shape with a size ranging from approximatively 14.13 to 21.26 nm. The presented silver nanoparticles exhibited strong antioxidant activity following a DPPH assay compared to ascorbic acid, with IC50 values of about 0.089 µg/mL and 22.54 µg/mL, respectively. The AgNPs showed higher antidiabetic capacities than acarbose, by inhibiting both alpha amylase and alpha glucosidase. The silver nanoparticles could affect various bacterial mechanisms of virulence, such as EPS production, biofilm formation and DNA damage. The silver nanoparticles showed no lysozyme activity on the cell walls of Gram-positive bacteria. The AgNPs also had a strong inhibitory effect on the Candida albicans virulence factor (extracellular enzymes, biofilm formation). The microscopic observation showed abnormal morphogenesis and agglomeration of Candida albicans exposed to AgNPs. The AgNPs showed no cytotoxic effect on human cells in an MTT assay. The use of novel silver nanoparticles is encouraged in the formulation of natural antimicrobial and antidiabetic agents.

The present paper described the first green synthesis of silver nanoparticles (AgNPs) from the extremophile plant Aeonium haworthii. The characterization of the biosynthesized silver nanoparticles was carried out by using UV-Vis, FTIR and STM analysis. The antioxidant, antidiabetic and antimicrobial properties were also reported. The newly described AgNPs were spherical in shape and had a size of 35–55 nm. The lowest IC50 values measured by the DPPH assay indicate the superior antioxidant behavior of our AgNPs as opposed to ascorbic acid. The silver nanoparticles show high antidiabetic activity determined by the inhibitory effect of α amylase as compared to the standard Acarbose. Moreover, the AgNPs inhibit bacterial growth owing to a bactericidal effect with the MIC values varying from 0.017 to 1.7 µg/mL. The antifungal action was evaluated against Candida albicans, Candida tropicalis, Candida glabrata, Candida sake and non-dermatophytic onychomycosis fungi. A strong inhibitory effect on Candida factors’ virulence was observed as proteinase and phospholipase limitations. In addition, the microscopic observations show that the silver nanoparticles cause the eradication of blastospores and block filamentous morphogenesis. The combination of the antioxidant, antimicrobial and antidiabetic behaviors of the new biosynthesized silver nanoparticles highlights their promising use as natural phytomedicine agents.

Palm (Phoenix dactylifera L.) is a specie cultivated in Mauritania. The present study is focused on the potential of the aqueous extract of the Pergularia tomentosa L., as biological control agent against Fusarium brachygibbosum, the main agent of the date palm stipe rot disease in Mauritania. Fungal pathogen was isolated from symptomatic date palm stipe rot disease in Mauritania. The morphological characterization and the molecular identification by sequencing ITS1, ITS2 5.8 RNAr region showed homology of 98% with Fusarium brachygibbosum strain UOA/ HCPF 16982 s during in vitro tests on leaves performed to verify its phytopathogenicity. The inhibitory effect of aqueous extract of Pergularia tomentosa L. on the phytopathogenic isolate (Fusarium brachygibbosum) of date palm, on mycelial growth and spore germination was observed. In fact, in vitro on PDA, mycelial growth ranged from 39.23 to 67.7%, depending on the concentration of the aqueous extract of P. tomentosa. The aqueous extract showed a reduction of spore germination varying from 5.3 to 23.8%. The minimum inhibitory concentrations varied from 1 to 100 mg/ml. The various extracts give high antioxidant activities exhibited by DPPH assay. Multi -variant analysis by PCA plot and the heatmap were done, to evaluate the correlation between the tested parameters. These results suggest the use of Pergularia tomentosa L., extract as a biological agent to control and reduce dam-age caused by Fusarium brachygibbosum.

The current study was conducted to investigate the chemical composition of Quercus canariensis flour acorns extracts as well as its biological activities in regards to the growing area using spectrophotometric and chromatographic techniques. The phenolic profile was composed of 19 compounds identified through HPLC-DAD analysis. Coumarin was the most abundant compound quantified in BniMtir, Nefza and ElGhorra and gallic (12.58-20.52 %), syringic (4.70-7.64 %) and trans-ferulic (2.28-2.94 %) acids were the abundant phenolic acids while kaempferol was the major flavonoid compounds quantified only in Quercus canariensis growing in BniMtir. On the other hand, Ain Snoussi acorn extract was characterized by its high content in luteolin-7-O-glucoside (58.46 %). The in-vitro antioxidant activities of the studied extracts were investigated and the results showed that Nefza ethanolic extract's has the highest activities. A bactericidal effect against Staphylococcus aureus was observed only by Elghorra population. On the other hand, Ain Snoussi acorn extract was efficient to inhibit growth of pathogenic bacteria, mentioned the highest activity against Escherichia coli. These results is the first study highlighted that zeen oak acorns are an excellent source of natural antioxidants and antibacterial compounds related to their lysozyme activity which could be exploited in the pharmaceutical and food sectors.

The present work aimed to develop rapid approach monitoring using a simple selective method based on a positive hemolysis test, oil spreading activity and emulsification index determinations. It is the first to describe production of biosurfactants (BS) by the endophytic Pantoea alhagi species. Results indicated that the new BS evidenced an E24 emulsification index of 82%. Fourier-transform infrared (FTIR) results mentioned that the described BS belong to the glycolipid family. Fatty acid profiles showed the predominance of methyl 2-hyroxydodecanoate in the cell membrane (67.00%) and methyl 14-methylhexadecanoate (12.05%). The major fatty acid in the BS was oleic acid (76.26%), followed by methyl 12-methyltetradecanoate (10.93%). Markedly, the BS produced by the Pantoea alhagi species exhibited antimicrobial and anti-biofilm activities against tested human pathogens. With superior antibacterial activity against Escherchia coli and Staphylococcus aureus, a high antifungal effect was given against Fusarium sp. with a diameter of zone of inhibition of 29.5 mm, 36 mm and 31 mm, obtained by BS dissolved in methanol extract. The DPPH assay indicated that the BS (2 mg/mL) showed a higher antioxidant activity (78.07 inhibition percentage). The new BS exhibited specific characteristics, encouraging their use in various industrial applications.

Extremophilic microorganisms, with their remarkable adaptability to harsh environments, hold immense potential for biotechnological and industrial applications. This study represents a pioneering investigation into the production and characterization of lipase and biosurfactants (BSs) by Halomonas zhanjiangensis. The GC-MS analysis of fatty acid profiles indicates that methyl 2-hyroxydodecanoate is the major component with 59.130%, followed by methyl 14-methylhexadecanoate (18.98%) and vaccenic acid (10.902%). The carbon sources and the composition of the culture medium were tested to optimize the lipase and biosurfactant productions. The strain Mn8 was able to produce one lipase with a molecular mass of 64 kDa. The optimum enzyme production was obtained at pH 5, pH 8, and 50 °C. The maximum BSs production obtained in the presence of LB auditioned with 1% sunflower oil. The FTIR analysis indicates that the BSs belong to the glucolipid type. The chemical composition of the BSs by GCMS shows that the major fatty acid is 2-Hendecanone C11 with 67.37%, followed by 2-Nonanone with 21.28%. The glycolipid possesses high bioemulsifying activities. In conclusion, based on the activity of the lipase at extreme conditions, associated with the bioemulsifying potential, we conclude that the novel strain Mn8 the development of industrial formulation.