ABSTRACT Due to ageing of the population, bone frailty is dramatically increasing worldwide. Although some therapeutic options exist, they do not fully protect or prevent against the occurrence of new fractures. All current drugs approved for the treatment of bone fragility target bone mass. However, bone resistance to fracture is not solely due to bone mass but relies also on bone ECM material properties, i.e. the quality of the bone matrix component. Here, we introduce the first-in-class unimolecular dual GIP/GLP-2 analogues, GL-0001, that activate simultaneously the glucose-dependent insulinotropic polypeptide receptor (GIPr) and the glucagon-like peptide-2 receptor (GLP-2r). GL-0001 acts synergistically through a cAMP-LOX pathway to enhance collagen maturity. Furthermore, in mice with ovariectomy-induced bone fragility, GL-0001 prevented excess trabecular bone degradation at the appendicular skeleton and also enhanced bone ECM material properties through reduction of the degree of mineralization and augmentation in enzymatic collagen crosslinking. These results demonstrate that targeting bone ECM material properties is a viable option to enhance bone strength and opens an innovative pathway for the treatment of patients suffering of bone fragility.

L’association de deux cancers chez un même patient est rare. Le plus souvent, le deuxième cancer est provoqué par l’immunodépression induite par les traitements (chimiothérapie, radiothérapie) de la première néoplasie. Le myélome multiple et le cancer du rein partagent des facteurs de risque (obésité, tabagisme, hypertension artérielle), et plusieurs cas d’associations de ces néoplasies ont été décrits dans la littérature. Nous rapportons pour la première fois deux cas cliniques d’association entre un myélome multiple et un carcinome rénal à cellules claires de découverte synchrone avec une rechute osseuse concomitante à distance du diagnostic initial. Des mécanismes physiopathologiques communs ont été décrits entre le carcinome rénal et le myélome multiple, notamment le rôle de l’interleukine 6 produite par les cellules rénales et qui stimule la prolifération des cellules myélomateuses. Les cliniciens doivent connaître la possibilité de cette association et en cas de rechute évidente de l’une des deux maladies rechercher systématiquement la rechute de l’autre maladie.

The involvement of a gut-bone axis in controlling bone physiology has been long suspected, although the exact mechanisms are unclear. We explored whether glucose-dependent insulinotropic polypeptide (GIP)-producing enteroendocrine K cells were involved in this process. The bone phenotype of transgenic mouse models lacking GIP secretion (GIP-GFP-KI) or enteroendocrine K cells (GIP-DT) was investigated. Mice deficient in GIP secretion exhibited lower bone strength, trabecular bone mass, trabecular number, and cortical thickness, notably due to higher bone resorption. Alterations of microstructure, modifications of bone compositional parameters, represented by lower collagen cross-linking, were also apparent. None of these alterations were observed in GIP-DT mice lacking enteroendocrine K cells, suggesting that another K-cell secretory product acts to counteract GIP action. To assess this, stable analogues of the known K-cell peptide hormones, xenin and GIP, were administered to mature NIH Swiss male mice. Both were capable of modulating bone strength mostly by altering bone microstructure, bone gene expression, and bone compositional parameters. However, the two molecules exhibited opposite actions on bone physiology, with evidence that xenin effects are mediated indirectly, possibly via neural networks. Our data highlight a previously unknown interaction between GIP and xenin, which both moderate gut-bone connectivity. © 2020 American Society for Bone and Mineral Research.

The combined presence of two cancers in a single patient is rare. Usually, the second cancer is caused by immunosuppression resulting from treatment (chemotherapy, radiotherapy) of the first neoplasia. Multiple myeloma and kidney cancer share similar risk factors (obesity, smoking, hypertension), and several cases involving the combination of these two neoplasias have been described in the literature. We are reporting, for the first time, two clinical cases involving the combined presence of multiple myeloma and clear cell renal cell carcinoma discovered synchronously, with concomitant bone recurrence some time after the initial diagnosis. Pathophysiological mechanisms have been described that are common to renal carcinoma and multiple myeloma; in particular, the role of interleukin-6, which is produced by the renal cells and stimulates the proliferation of myeloma cells. Clinicians must be aware of the possibility of this disease combination and, in the event of an obvious recurrence of one of these two diseases, should search systematically for recurrence of the other disease.

Bone fractures are common comorbidities of type 2 diabetes mellitus (T2DM). Bone fracture incidence seems to develop due to increased risk of falls, poor bone quality and/or anti-diabetic medications. Previously, a relation between gut hormones and bone has been suspected. Most recent evidences suggest indeed that two gut hormones, namely glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1), may control bone remodeling and quality. The GIP receptor is expressed in bone cells and knockout of either GIP or its receptor induces severe bone quality alterations. Similar alterations are also encountered in GLP-1 receptor knock-out animals associated with abnormal osteoclast resorption. Some GLP-1 receptor agonist (GLP-1RA) have been approved for the treatment of type 2 diabetes mellitus and although clinical trials may not have been designed to investigate bone fracture, first results suggest that GLP-1RA may not exacerbate abnormal bone quality observed in T2DM. The recent design of double and triple gut hormone agonists may also represent a suitable alternative for restoring compromised bone quality observed in T2DM. However, although most of these new molecules demonstrated weight loss action, little is known on their bone safety. The present review summarizes the most recent findings on peptide-based incretin therapy and bone physiology.

Suite à un repas, l’entrée de nutriments dans l’intestin grêle stimule les cellules entéroendocrines à secréter des hormones intestinales. Parmi, les hormones produites dans le duodénum, le polypeptide insulinotropique dépendant du glucose (GIP) est une hormone essentielle dans le métabolisme osseux. Le but de cette étude était d’élucider plus en détail le rôle du GIP dans l’histophysiologie osseuse en utilisant un modèle murin de délétion du GIP (GIP-GFP-KI). Huit souris males GIP-GFP-KI de 16 semaines ont été appariées avec 8 souris WT de même âge et de même sexe. Les microarchitectures trabéculaire et corticale ont été évaluées par microtomographie-X au tibia. La résistance osseuse a été quantifiée au fémur par flexion 3 points et sur coupes tissulaires par nanoindentation. La composition de la matrice a été déterminée par qBEI et par microspectroscopie infra-rouge à transformée de Fourier. Les souris GIP-GFP-KI présentent une diminution de la masse osseuse trabéculaire ainsi que des altérations de microarchitecture. De plus, la résistance osseuse du fémur est diminuée chez les souris GIP-GFP-KI. Les souris GIP-GFP-KI montrent une altération de la phase minérale de la matrice avec une diminution de l’intégrité du collagène (−30 %) et une augmentation de la glycation du collagène (24 %). Ces résultats supportent un rôle de la voie GIP/GIPR dans le contrôle de la qualité et de la résistance osseuse. Ceci est particulièrement important au vu des antagonistes du récepteur au GIP qui sont en cours de développement pour le traitement de l’obésité.

Glucose-dependent insulinotropic polypeptide (GIP) has been recognized in the last decade as an important contributor of bone remodelling and is necessary for optimal bone quality. However, GIP receptors are expressed in several tissues in the body and little is known about the direct vs indirect effects of GIP on bone remodelling and quality. The aims of the present study were to validate two new GIP analogues, called [D-Ala(2)]-GIP-Tag and [D-Ala(2)]-GIP(1-30), which specifically target either bone or whole-body GIP receptors, respectively; and to ascertain the beneficial effects of GIP therapy on bone in a mouse model of ovariectomy-induced bone loss. Both GIP analogues exhibited similar binding capacities at the GIP receptor and intracellular responses as full-length GIP(1-42). Furthermore, only [D-Ala(2)]-GIP-Tag, but not [D-Ala(2)]-GIP(1-30), was undoubtedly found exclusively in the bone matrix and released at acidic pH. In ovariectomized animals, [D-Ala(2)]-GIP(1-30) but not [D-Ala(2)]-GIP-Tag ameliorated bone stiffness at the same magnitude than alendronate treatment. Only [D-Ala(2)]-GIP(1-30) treatment led to significant ameliorations in cortical microarchitecture. Although alendronate treatment increased the hardness of the bone matrix and the type B carbonate substitution in the hydroxyapatite crystals, none of the GIP analogues modified bone matrix composition. Interestingly, in ovariectomy-induced bone loss, [D-Ala(2)]-GIP-Tag failed to alter bone strength, microarchitecture and bone matrix composition. Overall, this study shows that the use of a GIP analogue that target whole-body GIP receptors might be useful to improve bone strength in ovariectomized animals.