Several plant growth regulators applied to established sod driveways in an apple orchard suppressed growth of the ground cover sufficiently to eliminate one to three mowings. MH at 4.5 or 6.7 kg ai/ha applied spring and fall reduced the growth of a single species sod cover crop, ‘Kentucky 31’ tall fescue, the year after treatment. MH at both rates also reduced the dandelion population growing in the mixed species orchard sod. Paclobutrazol or EPTC applied in the spring before or during initial grass growth reduced dry matter production in the fescue sod cover crop and the number of mowings compared to the mowed and non-mowed control plots.
Foliar sprays of Promalin (gibberellins A4 + 7 + 6-benzylamino purine) applied to 1-year-old apple (Malus domestica Borkh.) trees at the 3–5-cm growth stage significantly increased lateral branching in 5 of 9 cultivars tested. Branching response ranged from 0% (‘Winter Banana’) to 131% (‘Starkrimson Delicious’) of the untreated controls. Total shoot growth was not consistently increased by 6-benzylamino purine or Promalin in tests over 3 years on spur and non-spur 1- and 2-year-old apple trees. Sprays of 50–300 mg l−1 Promalin were ineffective for increased branch development. Sprays of 300–500 mg l−1 increased total shoot numbers and reduced average shoot length. Sprays applied prior to new terminal growth in spring were ineffective. Treatment during periods of active shoot growth were generally effective, but periods of stress may have reduced response. Sprays were more effective for inducing lateral shoot formation than dormant heading or delayed dormant heading (pruning) (10 days after full bloom) in ‘Criterion Golden Delicious’. No difference in branching response was observed between BA and Promalin. Addition of a spray adjuvant (Buffer-X) did not affect branching response. Repeat annual single sprays of Promalin applied to dormant pruned trees were generally less effective for stimulating lateral branching than a single application in a given year. Phytotoxicity was associated with Promalin sprays at 300–500 mg l−1 on ‘Delicious’.
The degree of Cytospora infection, gumming, and internal discoloration was significantly influenced by the type of pruning cuts used on 10-year-old ‘Loring’ peach [ Prunus persica (L.) Batsch] trees. Time of pruning did not affect gumming or Cytospora infection. Pruning cuts which leave a raised collar of tissue at the branch junction resulted in the least infection, external dieback, and internal discoloration.
A developmental electron microscopic study of the parasitism of Rolylenchulus reniforrnis in resistant 'Peking' and susceptible 'Lee' soybeans was made during a 21-day period under controlled conditions. Within 2 days of inoculation, the nematode had penetrated the cortical cells to the endodermis where it inserted its stylet, secreted and initiated syncytial formation and cell hypertrophy. Syncytia primarily involved pericycle tissues and, to a lesser extent, xylem parenchyma and endodermis. When identifiable, the cell into which the nematode stylet was inserted to initiate syncytial development was endodermal. Susceptible tissues exhibited two basic phases of development during this infection period: (i) an initial phase represented by partial cell wail lysis and separation; and (ii) an anabolic phase, characterized by organelle proliferation and development accompanied by secondary wall deposits, which provided nutrition for sessile female development. The resistant or hypersensitive reaction (HR) lacked the anabolic phase found in the susceptible reaction, and was characterized by an extension and usually accelerated type of Iysis found in the first phase of the syncytial development. The HR was usually very evident 4 days after inoculation, and could be identified by an almost complete lysis of the cell walls and cytoplasm. The possibility that the initial cell of the developing syncytium or "prosyncyte" may influence a susceptible or resistant reaction is discussed. Successive stages of cell wall dissolution and the deposition of secondary cell walls are described.