Oral lichen planus is a chronic inflammatory disorder of the oral mucosa that represents T cell-mediated autoimmune diseases. The regulation and roles of carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1), a novel immune molecule, in the immunopathogenesis of T cell-mediated autoimmune diseases remain unclear. In the current paper, CEACAM1 was found to be overexpressed in peripheral T cells and epithelial cells in oral lichen planus patients. A fraction of infiltrating inflammatory mononuclear cells in the lamina propria of the oral lichen planus mucosa also expressed CEACAM1. Importantly, for the first time, CEACAM1 expression in T cells and in normal human oral keratinocytes was demonstrated to be regulated differently by osteopontin in vitro. Furthermore, the apoptosis of oral keratinocytes and activated T cells can be markedly suppressed by CEACAM1-specific monoclonal antibodies. In conclusion, OPN-regulated CEACAM1 expression may play a critical role in the immunopathogenesis of oral lichen planus.

目的 观察口腔扁平苔藓(OLP)患者外周血T细胞亚群的变化,并探讨其意义.方法 采用流式细胞仪检测237例口腔扁平苔藓患者(OLP组)、44例健康人(对照组)外周血T淋巴细胞亚群.结果 与对照组相比,OLP组外周血中CD3、CD4、CD4/CD8明显下降(P均<0.05).结论 OLP患者外周血T细胞亚群免疫功能明显降低,可能与OLP的发生有关.

Objective:To observe the correlation of inducible nitric oxide synthase(iNOS)with Bcl-2 in the golden hamster buccal pouch carcinogenesis induced hy dimcthyl-benzanthrance(DMBA).Methods:72 outbred six-week old Syrian gohten hamsters were randomly divided into experimental group(60 animals)and control group(12 animals).The pouches of the experimental hamsters were painted with 0.5% DMBA solution three times a week.Light microscope was used to observe the pathologic changes.Expressions of iNOS and Bel-2 were observed hy immunohistochemistry.Results: In oral carcinoma group iNOS and Bcl-2 protein expression were significantly increased compared with the normal or hyperplasia group(P0.05).There was complicated relationship between nitric oxide and Bel-2.Conclusion:Nitric oxide might suppress or induce cell apoptosis by regulating apoptotic signaling cascades in Bcl-2.

采用流式细胞学技术检测10例盘状红斑狼疮(DLE)和10例天疱疮患者外周血CD4+T细胞表面CD44的表达水平,ELISA法测定其血清骨桥蛋白(OPN)水平。结果天疱疮患者外周血CD4+T细胞表面CD44表达水平显著高于健康成人(P<0·01),DLE者CD4+T细胞表面CD44表达水平与健康成人比较差异无统计学意义;天疱疮与DLE患者血清OPN水平均显著升高,与健康成人比较,P<0·01。认为OPN在口腔黏膜病中的显著升高促进了疾病发生发展;其机制可能与CD4+T细胞表面OPN受体CD44与OPN结合触发的效应有关。

目的:研究Fas蛋白在口腔黏膜癌变过程中的表达及变化规律.方法:用DMBA诱导的金黄地鼠颊囊癌变模型,采用免疫组化SP法检测在黏膜上皮癌变过程(正常黏膜、单纯增生、轻度增生、中度增生、重度增生与鳞癌)中Fas蛋白的表达.结果:Fas在正常地鼠口腔黏膜中广泛表达,在黏膜癌变过程的不同阶段中Fas表达下调,尤其在鳞癌组织中明显降低(P＜0.05),且表达部位由细胞胞膜转向胞浆内.结论:Fas参与口腔黏膜肿瘤癌变过程,对口腔癌的早期诊断及治疗提供研究方向.

Objective: To investigate the clinical biological profiles, pathological diagnosis and treatment of verruciform xanthoma of the oral mucosa. Methods: The clinicopathological data of 6 cases with verruciform xanthoma of the oral mucosa were retrospectively analyzed, with follow-up. Results: Five patients were misdiagnosed before the operation. They were confirmed with intraoperative rapid pathological diagnosis and routine postoperative pathological diagnosis. The effective treatment was local excision. No case with recurrence or canceration was found in the follow-up. Conclusions: Misdiagnosis is common on the verruciform xanthoma. Application of the rapid pathological diagnosis during operation is needed when the suspectable malignancy occurs for fear of overtreatment. The prognosis is favourable after local excision.

Objective:To study the role of Runx2/cbfa1 in human tooth development.Method:The specimen of the human's tooth germ in different stages were made to observe the expressions of cbfa1 during tooth germ development by immunofluorescence method.Result:Cbfa1 had its own specific temporal-spatial pattern during tooth germ development.In bud stage,the factor expressed widely;in cap stage,the inner and outer enamel epithelium showed strong positive expressions,as well as the medium layer.While the dental papilla showed weak expression;in the early bell stage,preodontoblast expressed positively;in the medium and late bell stage,adamantoblast was positive while odontoblast was negetive.Conclusion:The expressions of Runx2/cbfa1 during tooth germ development indicates that this factor possibly plays a role in every stage of tooth germ development,participating the differentiation of kinds of cells and the hard tissue formation.

PURPOSE:To study the expression of Fas and bcl-2 in oral carcinogenesis.METHODS:Using immunohistochemical method,we studied the expression of Fas and bcl-2 in DMBA induced hamster baccal carcinogenesis including negative control (14), hyperkeratosis (7), epithelial dysplasia (mild 12, moderate 7, severe 9), oral squamous cell carcinoma(OSCC) (25). Non-parametric test was used for statistical analysis.RESULTS:Fas was expressed in negative control consistently except stratum basale,and there was down-regulation in oral dysplasia and OSCC (chi(2)=45.576, P<0.05).bcl-2 was rarely expressed in negative control, and there was up-regulation in oral dysplasia and OSCC (chi(2)=19.433, P<0.05).CONCLUSIONS:Fas and bcl-2 may play an important role in inhibiting apoptosis coordinately, resulting in the imbalance of cell proliferation and apoptosis, which may contribute to oral carcinogenesis.

Objective: To assess the role of nitric oxide in the golden hamster pouch carcinogenesis induced by 7,12-dim-ethylbenzanthracene( DMBA). Methods: Seventy-two outbred six-week old Syrian golden hamster were randomly divided into one experimental group (60 animals) and one control group (12 animals). The control group remained untreated. The pouches of the experimental group were painted with 0. 5% DMBA solution three times each week. Light microscope was used to observe the pathologic changes. The serum nitric oxide level was measured by chemical photometry. The expression activity of the inducible nitric oxide synthase was observed by immunohistochemistry. Results: In oral carcinoma group serum NO level and iNOS protein expression were significantly increased compared with the normal or hyperplasia group (P 0. 05) , No iNOS protein expression could be detected in the untreated pouches. There was a significant relationship between iNOS protein expression and serum NO level (rs =0.590, P0.01). Conclusion: Increased NO level could contribute to the mechanism for experimentally induced oral carcinogenesis.

OBJECTIVE:To investigate the expression and alteration (including homozygous deletion and mutation) of MTS1 gene in precancerous lesions and squamous cell carcinomas (SCC) of oral mucosa, and to analyse the function of MTS1 gene alteration in oral mucosal carcinogenesis.METHODS:The expression of p16 protein produced by MTS1 gene was examined with immunohistochemical SP method in 10 normal oral mucosas, 30 precancerous lesions (10 mild, 10 moderate and 10 severe dysplasia respectively) and 45 squamous cell carcinomas (SCCI18, SCCII 19, SCCIII 8). The deletion and mutation of exon1 and exon2 of MTS1 gene were examined with methods of PCR and SSCP in these same samples.RESULTS:All the precancerous lesions had p16 protein expression and no alteration of MTS1 gene. In SCC, the positive rate of p16 protein was 60.0% with 72.2% in SCCI, 57.9% in SCCII, 37.5% in SCC III, and there were no significant difference among the three groups by chi2 test (P>0.05). Gene homozygous deletion of exon1 and/or exon2 was detected in 10 cases, and gene mutation in 4 cases. The whole rate of gene alteration was 31.1% (14/45). The MTS1 gene alteration rate was 27.8% in SCCI, 31.6% in SCCII, 37.5% in SCC III and there was also no significant difference among the three groups by chi2 test (P>0.05). In SCC with local lymph nodes metastasis, MTS1 alteration rate was 57.1%, while in SCC with no lymph nodes metastasis was 8.3%, and there was significant difference by chi2 test (P<0.05).CONCLUSIONS:MTS1 gene alteration is not an early event in the carcinogenesis of oral mucosa and can not be used as a biology mark to examine oral precancerous lesions. MTS1 gene may play a certain role in the progression of oral squamous cell carcinomas.

OBJECTIVE:The purpose of this study was to observe the biological characteristics of cultured Periosteal-derived Osteoblasts (POBs) preserved in liquid nitrogen in vitro, and to preliminarily study the osteogenetic capability of bioactive glass ceramic (BGC) combined with POBs.METHODS:The cryopreserved cells were cultured in DMEM medium and examined by morphological and histological assay. The POBs growing well in vitro were seeded into the porous BGC materials. A week later, the combined materials were implanted into the bone defects of rabbits' mandibular, the control groups were implanted into the single BGC (no cells). After 2, 4, 8, 12 weeks of operation, the specimens were respectively excised and examined by X-ray and histological chemistry.RESULTS:The cryopreserved POBs grew well in vitro and also had the tipical characteristics of mature osteoblasts. Cultured with BGC materials, the cells could attach, grow and proliferate well on the surface of most endoporous. After 4 weeks of operation, the transplanted osteoblasts began to form new osteoid or bone tissue in most pores of implanted BGC, and the bone defects were repaired better and earlier.CONCLUSION:It was practical to use the cryopreserved osteoblasts for further study on bone tissue engineering. It suggested that the "life active" bone would get more application and play a more important role in bone restoration and reconstruction.

目的:寻找从兔面神经中快速分离和纯化许旺氏细胞的方法.方法:以胰蛋白酶和胶原蛋白酶对神经进行消化,找出最佳的消化时间后,采用多聚赖氨酸特异性粘附法纯化许旺氏细胞.结果:神经消化时间以45～60min时所得到的细胞数量最多,经粘附处理后,排除了成纤维细胞,使许旺氏细胞的纯度明显增加.结论:采用酶消化法和L-多聚赖氨酸特异性粘附法,可在较短时间内获得高纯度的许旺氏细胞.