This study investigates COVID-19 outcomes and immune response in chronic myeloid leukemia (CML) patients post-SARS-CoV-2 vaccination, comparing effectiveness of various vaccine options. Data from 118 CML patients (85 in Brazil, 33 in the US) showed similar infection rates prior (14% Brazil, 9.1% US) and post-vaccination (24.7% vs. 27.3%, respectively). In Brazil, AstraZeneca and CoronaVac were the most commonly used vaccine brands, while in the US, Moderna and Pfizer-BioNTech vaccines dominated. Despite lower seroconversion in the Brazilian cohort, all five vaccine brands analyzed prevented severe COVID-19. Patients who received mRNA and recombinant viral vector vaccines (HR: 2.20; 95%CI 1.07-4.51; p < .031) and those that had achieved at least major molecular response (HR: 1.51; 95% CI 1.01-3.31; p < .0001) showed higher seroconversion rates. Our findings suggest that CML patients can generate antibody responses regardless of the vaccine brand, thereby mitigating severe COVID-19. This effect is more pronounced in patients with well-controlled disease.

Design and Setting: Retrospective cohort study conducted at the Hospital de Especialidades Centro Médico Nacional de Occidente, including patients diagnosed with CML and treated with TKIs since diagnosis.Molecular monitoring of the BCR-ABL1 fusion gene transcript was performed using quantitative polymerase chain reaction (qPCR) according to the International Scale.Patients who achieved a deep molecular response for at least 2 years were included.Patient Characteristics and Interventions: A total of 17 patients with CML who met the inclusion criteria were studied, including 6 males and 11 females, with a mean age of 36 years.The average time in deep molecular response with TKIs was 4 years.Outcomes: The primary outcome evaluated was treatment-free remission at 24 months after discontinuation of TKIs.Results: The overall treatment-free remission rate at 24 months was 41%.The variable associated with treatment-free remission was female sex, while no other clinical or laboratory variables showed statistically significant differences.Conclusions: With more prolonged and sustained deep molecular responses, it is possible to achieve more durable treatmentfree remissions and eventually achieve the definitive discontinuation of TKIs.

Limb ulcers are one of the most debilitating complications of Sickle Cell Diseases (SCD), greatly limiting the quality of life of these patients. Although Hydroxyurea (HU) is a fundamental element in the management of patients with SCD, its role of in the emergence or worsening of limb ulcers in SCD patients is still a matter of debate.

Topic: 8. Chronic myeloid leukemia - Clinical Background: Patients with hematologic malignancies have displayed lower rates of seroconversion to SARS-CoV-2 vaccines. Hematologic malignancies are a heterogeneous group of disorders and there is limited data concerning the immunogenicity post-vaccination among chronic myeloid leukemia (CML) patients, overall and by vaccine type. Aims: To analyze the seroconversion rates following two and three doses of five different SARS-CoV-2 vaccines among CML patients. We also explore the association between antibody responses, tyrosine kinase inhibitors (TKIs) and treatment response. Methods: We evaluated a prospective cohort of CML patients from two CML reference centers from Brazil and USA who received at least two doses of the SARS-Cov-2 vaccine. Blood samples were collected to measure SARS-CoV-2 IgG antibodies and neutralizing antibodies (nAB). All patients completed a health-related questionnaire. Results: Between August 2021 and December 2022, 135 CML patients were enrolled (102 in Brazil and 33 in USA). Demographics and clinical features of patients from both centers were similar, with 59% and 57% male patients, respectively (p= 0.90). Median age was 58 (33-85) and 64 (26-80) years (p= 0.82). All patients from the Brazil cohort were Hispanic, while only 7.5% of the USA group self-reported this ethnicity (p=0.001). Most patients were in the chronic phase at diagnosis (96% vs. 97%, p=0.85) and at enrollment (98% vs. 100%, p=0.42). The majority were taking TKIs, while 11% were in treatment-free remission (87% vs. 94%; 13% vs. 6%, respectively; p=0.28). In the Brazilian cohort 60% were receiving imatinib, 37% second-generation TKI (Dasatinib, Nilotinib, Bosutinib), and 2% third generation (Ponatinib) in the US cohort 30% received imatinib, 61% second-generation and 9% third-generation (Ponatinib, Asciminb) (p<0.0001). Response status was similar between cohorts, with most patients with at least major molecular response (MMR) (82% vs. 73%, p=0.24). Regarding SARS-CoV-2 vaccines, AstraZeneca and CoronaVac were the most common brands of initial vaccination schedule applied in the Brazilian patients (66.5% and 29%, respectively), while Moderna and Pfizer-BioNTech were most common in the US (64% and 33%). The median interval of days between the second dose and the first sample collection was 96 days (22-697). Prior to SARS-CoV-2 vaccination, 13% had experienced COVID-19 whilst 26% acquired it after vaccination. There were no severe cases of COVID-19 after vaccination. Independent predictors for higher seroconversion rates were the type of the applied vaccines and the current CML response to the treatment. Higher seroconversion rates were observed in mRNA (Moderna and Pfizer-BioNTech) and recombinant viral vector (AstraZeneca and Janssen) compared to inactivated virus (CoronaVac) (HR: 2.20; 95%CI 1.07-4.51; p<0.031). Patients with MMR or better had significantly higher seroconversion rate compared to no MMR (HR: 1.51; 95% CI 1.01-3.31; p<0.0001). Summary/Conclusion: Patients receiving mRNA and recombinant viral vector vaccines and those with at least MMR have the highest seroconversion rate after vaccination. Still, our results suggest that all vaccine types may prevent severe COVID-19 in CML patients. Keywords: COVID-19, Chronic myeloid leukemia, Cancer vaccine, Vaccination