A perfused locust thoracic muscle preparation was used to study the effects of octopamine on the content of fructose 2,6-bisphosphate, a potent activator of the glycolytic key enzyme phosphofructokinase, in the flight muscle of the locust Locusta migratoria. Perfusion with octopamine resulted in a significant increase in fructose 2,6-bisphosphate in flight muscle. The naturally occuring d-isomer was more potent than both d,l-octopamine and l-octopamine and gave a significant effect at 10−7 M, a concentration obeserved in the haemolymph of flying locusts. The adipokinetic hormones AKH I and AKH II of Locusta had no effect on fructose 2,6-bisphosphate in perfused flight muscle. Taurine which can be found in high concentrations in locust flight muscle was also ineffective. Electrical stimulation of perfused flight muscle (at 2 or 4 Hz for 15 min) caused the content of fructose 2,6-bisphosphate in flight muscle to decrease. Electrical stimulation, however, could not reverse the effect of octopamine added to the perfusion medium. It is suggested that octopamine-stimulated elevation of fructose 2,6-bisphosphate contributes to activation of glycolysis during locust flight.

The biogenic amine octopamine was injected into the haemolymph of 20-days old male locusts,Locusta migratoria, and the content of fructose 2,6-bisphosphate, a potent activator of glycolysis, was measured in the flight muscle after various time. Octopamine brought about a transient increase in fructose 2,6-bisphosphate. After the injection of 10 μl of 10 mmol·l-1d, l-octopamine fructose 2,6-bisphosphate was increased by 61% within 2 min. Ten minutes after the injection fructose 2,6-bisphosphate was increased to 6.71±0.89 nmol·g-1 flight muscle, almost 300% over the control value. Flight caused fructose 2,6-bisphosphate in flight muscle to decrease, but this decrease was counteracted by octopamine injected into the haemolymph of flying locusts. Octopamine and fructose 2,6-bisphosphate may act as signals to stimulate the oxidation of carbohydrate and to integrate muscle performance and metabolism. This mechanism appears particularly significant in the initial stage of flight when carbohydrates are the main fuel.

Conference Article| August 01 1993 A model for the catalytic mechanism of prostaglandin endoperoxide synthase Hans H. Ruf; Hans H. Ruf 1Fraunhofer-Institut für Biomedizinische Technik, and Fachrichtung Medizintechnik der Universität des Saarlandes Ensheimer Straşe 48, D-66386 St. Ingbert, Germany Search for other works by this author on: This Site PubMed Google Scholar Ute Raab-Brill; Ute Raab-Brill 1Fraunhofer-Institut für Biomedizinische Technik, and Fachrichtung Medizintechnik der Universität des Saarlandes Ensheimer Straşe 48, D-66386 St. Ingbert, Germany Search for other works by this author on: This Site PubMed Google Scholar Cornelia Blau Cornelia Blau 1Fraunhofer-Institut für Biomedizinische Technik, and Fachrichtung Medizintechnik der Universität des Saarlandes Ensheimer Straşe 48, D-66386 St. Ingbert, Germany Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1993) 21 (3): 739–744. https://doi.org/10.1042/bst0210739 Article history Received: April 01 1993 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation Hans H. Ruf, Ute Raab-Brill, Cornelia Blau; A model for the catalytic mechanism of prostaglandin endoperoxide synthase. Biochem Soc Trans 1 August 1993; 21 (3): 739–744. doi: https://doi.org/10.1042/bst0210739 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: COX, cyclo-oxygenase, PGG prostaglandin G, PGG2, prostaglandin G2, PGH, prostaglandin H, POD, hydroperoxidase, PPIX, protoporphyrin IX © 1993 Biochemical Society1993 Article PDF first page preview Close Modal You do not currently have access to this content.

Conference Article| April 01 1991 Regulation of fructose 2,6-bisphosphate in perfused flight muscle of the locust, Locusta migratoria: The effect of octopamine CORNELIA BLAU; CORNELIA BLAU 1Institut für Zoologie, Johannes Gutenberg-Universität Mainz, Saarstrasse 21, D-6500 Mainz, F.R.G. Search for other works by this author on: This Site PubMed Google Scholar DAVID J. CANDY; DAVID J. CANDY *School of Biochemistry, University of Birmingham, P.O. Box 363, Birmingham B15 2TT, U.K. Search for other works by this author on: This Site PubMed Google Scholar GERHARD WEGENER GERHARD WEGENER 1Institut für Zoologie, Johannes Gutenberg-Universität Mainz, Saarstrasse 21, D-6500 Mainz, F.R.G. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1991) 19 (2): 136S. https://doi.org/10.1042/bst019136s Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation CORNELIA BLAU, DAVID J. CANDY, GERHARD WEGENER; Regulation of fructose 2,6-bisphosphate in perfused flight muscle of the locust, Locusta migratoria: The effect of octopamine. Biochem Soc Trans 1 April 1991; 19 (2): 136S. doi: https://doi.org/10.1042/bst019136s Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1991 Biochemical Society1991 Article PDF first page preview Close Modal You do not currently have access to this content.

Chromatographic modifications have been made in the tetracycline fluorescence test for gastric cancer designed to improve its reliability. In limited studies using these modified procedures, tetracycline fluorescence was identified in the gastric sediments of 10 (91 %) of 11 patients with cancer of the stomach. By contrast, tetracycline fluorescence was not found on paper chromatography in the gastric sediments of 62 subjects with either normal stomachs or various benign lesions of the stomach. These promising but preliminary results are reported to encourage trial of these methods in areas where gastric cancer is prevalent.