Conventionally, there are two basic configurations for microbial fuel cell (MFC), one with anode and cathode chambers, as known as dual-chamber MFC, one with only anode chamber and a air-cathode, called single-chamber MFC. However, electrode materials' complex configuration and high resistance limit their practical applications in power generation and wastewater purification. To this end, we constructed three MFCs using carbon felt, Ni/Ti, and Ag-Ni/Ti as anodes based on our previously constructed integrated chamber-free MFC (iMFC). After 21 days of operation, the Ag-Ni/Ti-MFC performed best in bioelectricity output and removal of target pollutants. The bioelectricity output parameters were an open-circuit voltage of 0.773 V, a maximum current density of 552.1429 mA/m2, and a power density of 426.8064 mW/m2. Furthermore, the MFC achieved removals of 67.90% for the chemical oxygen demand (COD), 55.20% for total nitrogen (TN), and 61.83% for ammonia nitrogen (NH3-N). Introducing silver nanoparticles to the transmembrane and outer membrane boosted the charge extraction efficiency in the MFCs. Moreover, 16S rDNA analysis indicated that typical electricity-producing bacteria, including Comamonas, Paraclostridium, and Asaccharospora, had a higher relative abun-dance in the Ag-Ni/Ti-MFC anode than in other anodes. Overall, the surface structure of different anode ma-terials plays a critical role in bioelectricity generation and wastewater purification using iMFC by affecting the mass transfer of substrates and metabolites.

Although microbial-induced carbonate precipitation (MICP) technology is both environmentally friendly and cost-effective, its efficiency is constrained by challenges such as low bacterial activity and heavy metal stress. This study explored the enhancement of mineralization efficiency by incorporating zinc (Zn) into the cultivation system of carbonate-mineralized bacteria. All Zn salts at a concentration of 30 μmol/L significantly enhanced the density and heavy metal resistance of bacterial cells, while also promoting CO2 hydration efficiency. The activities of urease and carbonic anhydrase (CA) were significantly elevated after treatment with 30 μmol/L ZnCl2 and Zn(C3H5O3)2 (ZnL) compared to the control. The results from qRT-PCR and ELISA confirmed that ZnL exhibited a stable biological effect on CA gene expression. Through the analysis of surface chemistry of cells and the subcellular distribution pattern of cadmium (Cd), it was observed that Zn supplementation maintained the cell surface stability and strengthened the cellular barrier against Cd uptake. SEM, FTIR and XRD results further confirmed that Zn supplementation significantly increased the complexity of the mineral morphology, resulting in a more stable crystal structure of CdCO3. This study offers additional theoretical and technical backing, opening a new avenue for the practical application of MICP technology in heavy metal remediation.

Long-term vanadium titanomagnetite (VTM) mining in Panzhihua, China, leads to severe metal pollution in the adjacent agricultural soil, which causes great environmental and health concerns. In this study, the farmland soils with different plants (Grape, Mango, Tobacco, Corn) were collected near titanium-magnetite tailing in the Panzhihua area to investigate the metals (V, Fe, Mn, Ti) contamination and the related microbial response. The study area had a lateritic red soil type and was irrigated with water from a nearby river. The soils with cultivation showed an increase in the pH and a decrease in electrical conductivity (EC) compared with no-till land soil (control). This study's soils presented an average total V at 233.57 mg/kg, with no significant difference (p > 0.005) in different farmland soils. The planted soil presented more proportion of available Fe and Mn than unplanted soil, but there was no significant difference in the proportion of available V and Ti. Soils with different plants showed varied available metals. Especially the percentage of available V of the total (A-V/V) was highest in the grape planting area, while tobacco planting significantly decreased the availability of V and Ti. Furthermore, the planted soils showed 2.33-3.11 times, 9.50-37.89 times, 0.11-1.20 times, and 5.43-26.46 times higher urease, dehydrogenase, and sucrase activities, and acid phosphatase than the unplanted ones, respectively. The metal resistance genes (van) and integration genes (intl1) were enriched in planted soil. The dominant genera, including Nocardioides and Sphingomonas, were positively correlated with available V, Ti, Mn, and acid phosphatase activity (p < 0.05), which may participate in the enrichment van and intl1. Such knowledge reveals the impact of VTM mining on the agricultural ecological environment and potentially contributes to the risk evaluation of such influenced lands.

Fungal endophytes not only tolerate and activate Cd in soil but also promote host growth, yet its Cd activation capacity and mechanism remain unrevealed. Our previous study isolated a robust endophyte Bacillus thuringiensis L1 from Coprinus comatus fruiting body with splendid Cd resistance and activation abilities under laboratory conditions. In this study, those peculiarities were investigated in the actual soil environment. L1 could significantly increase the soil bioavailable Cd content and effectively compensate for alkali-hydro nitrogen losses and microbial inhibition caused by Cd. Furthermore, L1 inoculation improved the soil's bacterial community structure and increased the relative abundance of Cd-resistant bacteria, such as Actinobacteria, Chloroflexi, Acidobacter, and Firmicutes, closely associated with the soil enzyme activity shift. The genome sequencing analysis revealed the presence of genes related to growth promotion, resistance to Cd stress, and Cd activation, which were significantly up-regulated under Cd stress. Notably, L1 mainly activates Cd in soil by secreting citric acid, succinic acid, siderophore, and soluble phosphorus substances to chelate with Cd or dissolve bounded Cd. Meanwhile, the metal-responsive transcription repressor (CadC) and the Cd-translocating protein P-type ATPase (CadA) can help the L1 to suppress the toxicity of Cd. Those results help to unveil the possible mechanism of L1 in Cd-contaminated soil remediation, providing a clear strategy for Cd bio-extraction from soil.

Heavy metals (HMs) in husbandry waste have become a serious concern. To understand the impact of bioaugmentation on HMs-influenced composting, HMs-influenced swine manure was composted with nitrogen-retaining microbial agents (NRMA). Compared with control (CG), compost with NRMA (EG) presented a significantly lower HOAc extractable Cd (23.10%), Cu (48.15%), Cr (82.79%), Pb (4.49%), and Zn (29.15%) (P < 0.05). EG also showed 5 days longer high-temperature period during composting. After composting, EG had a 4.14% higher TN (27.93 g/kg) than CG (26.82 g/kg) but a 32.26% lower NH4+-N. The change in pH and NH4+-N driven by microbial activity was found the main reason for HMs bioavailability decrease. NRMA greatly strengthened the enrichment of HMs resistant bacteria such as Actinobacteriota and Chloroflexi in EG, whose abundance increased by 21.23% and 2473.75% compared with day 0 after composting, respectively. HMs resistance genes, such as copA (increased by 936.84%), czcA (62.95%), cadC (63.06%), and pbrT (684.08%), and chrB (16.89%), also elevated in EG than CG. Eventually, NRMA was able to regulate the microbial composition of manure composting under HMs impact, reduce HMs toxicity, and enhance composting efficiency, which should be considered for the safe disposal of such polluted waste.

Commercially available dissolved oxygen (DO) sensors are hardly suitable for stereoscopic and precise DO monitoring due to their design, high cost and susceptibility to matrix effects. Here, we have developed a DO biosensor based on an integrated chamber-free microbial fuel cell (DOMFC) as the core and a Raspberry Pi microcomputer as the data acquisition system. This biosensor is low cost, readily available and compact in configuration. To this end, stable microbial biofilms with oxygen gradients were established on bioaffinity aluminium foam as anode. The DOMFC sensor has a low internal resistance (9.62 Ω) that can respond to DO changes in less than one minute and produce a reliable voltage signal to record DO (0.15-9.5 mg/L) under challenging conditions. After training the GA-BPNN model with multidimensional data by automatically applying a data fusion strategy from multiple sources, accurate DO predictions (R2 = 0.997, RMSE = 0.0447, MAE = 0.0401) were obtained. The DOMFC sensor and the prediction model showed excellent agreement (R2 = 0.954) in complex natural applications (different pH values, conductivities, water temperatures, etc.), covering a wide range of applications. Since the sensor mini-monitoring system is inexpensive and easy to make and use on a large scale, it is a promising alternative for oxygen measurements in both natural and artificial waters.

Electrochemical bacteria Shewanella oneidensis MR-4 (MR-4) was used to biologically generate cadmium sulfide (bio-CdS) nanocrystals and construct a self-assembled intimately coupled photocatalysis-biodegradation system (SA-ICPB) to remove cadmium (Cd) and tetracycline hydrochloride (TCH) from wastewater. The characterization using EDS, TEM, XRD, XPS, and UV-vis confirmed the successful CdS bio-synthesis and its visible-light response capacity (520 nm). 98.4% of Cd2+ (2 mM) was removed during bio-CdS generation within 30 min. The elec-trochemical analysis confirmed the photoelectric response capability of the bio-CdS as well as its photocatalytic efficiency. Under visible light, SA-ICPB entirely eliminated TCH (30 mg/L). In 2 h, 87.2% and 43.0% of TCH were removed separately with and without oxygen. 55.7% more chemical oxygen demand (COD) was removed with oxygen participation, indicating the degradation intermediates elimination by SA-ICPB required oxygen participation. Biodegradation dominated the process under aerobic circumstances. Electron paramagnetic resonance analysis indicated that h+ and .O2- played a decisive role in photocatalytic degradation. Mass spec-trometry analysis proved that TCH was dehydrated, dealkylated, and ring-opened before mineralizing. In conclusion, MR-4 can spontaneously generate SA-ICPB and rapidly-deeply eliminate antibiotics by coupling photocatalytic and microbial degradation. Such an approach was efficient for the deep degradation of persistent organic pollutants with antimicrobial properties.

Antibiotic residue in husbandry waste has become a serious concern. In this study, contaminated chicken manure composting was conducted to reveal the bioaugmentation effect on tetracyclines residue and antibiotics resistance genes (ARGs). The bioaugmented composting removed most of the antibiotics in 7 days. Under bioaugmentation, 96.88 % of tetracycline and 92.31 % of oxytetracycline were removed, 6.32 % and 20.93 % higher than the control (P < 0.05). The high-temperature period was the most effective phase for eliminating antibiotics. The treatment showed a long high-temperature period (7 days), while no high-temperature period was in control. After composting, the treatment showed 13.87 % higher TN (26.51 g/kg) and 13.42 % higher NO3--N (2.45 g/kg) than control (23.28 and 2.16 g/kg, respectively) but 12.72 % lower C/N, indicating fast decomposition and less nutrient loss. Exogenous microorganisms from bioaugmentation significantly reshaped the microbial community structure and facilitated the enrichment of genera such as Truepera and Fermentimonas, whose abundance increased by 71.10 % and 75.37 % than the control, respectively. Remarkably, ARGs, including tetC, tetG, and tetW, were enhanced by 198.77 %, 846.77 %, and 62.63 % compared with the control, while the integron gene (intl1) was elevated by 700.26 %, indicating horizontal gene transfer of ARGs. Eventually, bioaugmentation was efficient in regulating microbial metabolism, relieving antibiotic stress, and eliminating antibiotics in composting. However, the ability to remove ARGs should be further investigated. Such an approach should be further considered for treating pollutants-influenced organic waste to eliminate environmental concerns.

Early blight caused by Alternaria solani is a serious threat to crop production. In this study, the synergistic antagonism mechanism of a biocontrol consortium (including Bacillus subtilis J3 and Pseudomonas fluorescens J8) isolated from the tomato rhizosphere against A.solani was investigated. The consortium (109 CFU/mL, J3:J8(v/v) = 2:3) efficiently inhibited A.solani growth with an inhibition rate of 94.10

The urgency to combat antibiotic-resistant bacterial infections requires new antibacterial materials and methods. Utilizing Shewanella onesidensis metabolism, biological zinc/copper sulfide (bio-ZnS/CuS) composites with excellent visible-light photocatalysis effects and broad-spectrum antibacterial activity were synthesized. During synthesis, over 95% of heavy metal ions were recovered from wastewater through co-precipitation to form metal sulfides. The biocomposite with Zn/Cu ratio at 1/9 (Zn1S/Cu9S) showed the best photocatalytic performance. Under visible-light catalysis of Zn1S/Cu9S, 98.02 +/- 0.13% of methylene blue and 81.74 +/- 2.12% of rhodamine B were rapidly removed, while sterilization rates exceeded 99.99% against Escherichia coli and 99.98% against Staphylococcus aureus. The biocomposite showed a nanostructure with sizes between 5 and 20 nm. Character-izations including X-ray photoelectron spectroscopy, UV-Vis absorption spectra, and photoluminescence spectra proved its excellent visible light response capacity (>400 nm) and energy utilization efficiency. Scavenging experiments demonstrated photogenerated holes and hydrogen peroxide as the major reactive oxygen species (ROS) that induce bacterial death. Toxicological studies revealed that ROS attacked bacterial cells by damaging membranes, inhibiting energy metabolism, breaking the antioxidant defense system, and compromising DNA integrity. This research presents innovative solutions for tackling bacterial infections and heavy metal contamination through the advancement of microbial synthetic functional nanomaterials.

Early blight of tomato caused by Alternaria solani results in significant crop losses. In this study, Bacillus subtilis J3 and Pseudomonas fluorescens J8 were co-cultured as a synthetic microbial community (BCA) for synergistic biocontrol of A. solani, and the inhibition mechanism was investigated. BCA presented an inhibition ration against A. solani at 94.91 • Bacillus and Pseudomonas present a synergistic biocontrol effect against A. solani. • Biocontrol prevents pathogen damage and improves tomato growth and systemic resistance. • Beneficial bacteria thrive in the rhizosphere is the key to microbial regulation.

Due to high oil content and complex composition, Kitchen waste (KW) is refractory for biotreatment and easily causes environmental burden, especially in Sichuan area with greasy cooking habits. To facilitate KW composting, a nitrogen-retaining & decomposing-promoting microbial agent (NRDPMA) was constituted with functional bacteria to decompose refractory substances, retain nutrients, and reduce gas emission. With NRDPMA, high-temperature period was achieved 2 days earlier and extended by 6 days, shorting the process by 15 days. 36.57% less NH3 and 22.30% less H2S were released. The total nitrogen of the compost product was increased by 17.14%. Functional bacteria in NRDPMA (e. g. Bacillus, Pseudomonas, and Oceanobacillus) displayed obvious advantages throughout composting, which reshaped microbial community composition and drove material transformation. PICRUST2 prediction confirmed a higher gene occurrence related to metabolism of lipid, lignocellulose, and nitrogen. The great decomposing and transformation capacity made NRDPMA an alternative to improve the efficiency of composting on recycling such lipid-containing waste. And functional bacteria targeting refractory components should be included for biotreatment of waste. This study should give a new perspective on industrial KW composting.

Bioreduction is an efficient approach to in-situ remediate Cr(VI)-contaminated soil, but further strengthening methods are still urgently needed. Herein, a novel immobilized biocomposite (B-HA-VE-SA) was successfully synthesized by embedding a efficient strain Bacillus sp. CRB-7 with humic acid (HA) combined vermiculite (VE) and sodium alginate (SA). The performance and enhancement mechanism of the immobilized biocomposite on remediating Cr(VI)-contaminated soil were also investigated by analyzing the whole-genome of CRB-7, Cr(VI) detoxification, soil microecological regulation, and subsequent crop growth response. Genomic annotation demonstrated that CRB-7 contains multiple genes contributed to Cr(VI) tolerance, Cr(VI) reduction and other metals resistance. Results showed that embedded CRB-7 biocomposites exhibited more effective reduction of Cr (VI) in soil compared with control and free CRB-7 treatment, especially B-HA-VE-SA achieved the highest Cr(VI) removal efficiency (96.18%) and the residual Cr proportion (49.04%) via multiple mechanisms including carrier effects, nutrient sustained-release, and electron-shuttle effect enhanced the bioremediation process. Furthermore, the synergies of CRB-7 and immobilizers (HA, VE and SA) significantly improved soil microecology (soil enzyme activities, microbial quantity and diversity), and engendered the evolution of microbial community composition and functional pathways. Consequently, pot experiments (Brassica napus L.) verified the plant-growth-promoting (12.00-18.00% and 43.82-69.00% higher in emergence rate and biomass) and Cr-accumulation-reducing effects (19.47-91.09% and 29.11-89.80% lower in root and aerial parts) of free and immobilized CRB-7. Taken together, these findings highlighted the superiority of B-HA-VE-SA in simultaneous remediation, microecological improvement and safe utilization of Cr(VI)-contaminated soil.

In this study, a lipid degrading microbe consortium (LDMC) was assembled to improve the performance of acti-vated sludge (AS) on cooking wastewater purification. LDMC can rapidly degrade high-level oil (efficiency beyond 93.0% at 5.0 g/L) as sole carbon source under various environmental conditions (10.0-45.0 degrees C, pH 2.0-12.0). With LDMC inoculation, AS' water treatment performance was significantly enhanced, which removed 36.10 and 48.93% more chemical oxygen demand (COD) and ammonia nitrogen from wastewater than control. A better set-tling property and smaller bulking risk were found with LDMC inoculation, indicated by a lower SV30 and SVI index but a higher MLSS. By GC/MS analysis, a gradual degradation on the end of the fatty acid chain was suggested. LDMC inoculation significantly changed AS's microbial community structure, improved its stability, decreased the micro-bial community diversity, facilitated the enrichment of lipid degraders and functional genes related to lipid bio-degradation. Lipid degraders including Nakamurella sp. and Stenotrophomona sp., etc. played a crucial role during oil degradation. Sludge structure maintainers such as Kineosphaera sp. contributed largely to the stability of AS under exogenous stress. This study provided an efficient approach for cooking wastewater treatment along with the underlying mechanism exploration, which should give insights into oil-containing environmental remediation. (C) 2021 Published by Elsevier B.V.

An integrated chamber-free microbial fuel cell (iMFC) was constructed, which can be directly used for wastewater purification and energy recovery. The iMFC demonstrated potential electricity generating ability, producing 123.02 mW m- 2 (688.90 mW m-3) with an open-circuit voltage of 0.75 V and current discharge of 294.32 mAh. Cyclic voltammetry analysis indicated that the electricity storage capacity contributed by pseudocapacitance effect enhanced power generation of iMFC. Electrochemical impedance analysis revealed a low internal resistance (60.20 omega), mainly related to internal diffusion. In addition, iMFC had an excellent chemical oxygen demand (COD) removal capacity, removing 81.20% of wastewater COD in 48 h, with optimum coulombic efficiency of 23.07%. The high-throughput sequencing analysis (16S rRNA/16S rDNA) indicated that Comamonas, Delftia, and Flavobacterium were dominant on both anode and cathode with varying abundance. Additionally, Pseudomonas and Clostridium_sensu_stricto_13 contributed as important exoelectrogens. With concise architecture, the iMFC could be easily integrated and used in existing wastewater treatment plants without further construction, making it feasible for industrial application.

In arable soils co-influenced by mining and farming, soil bacteria significantly affect metal (Cadmium, Cd) bioavailability and accumulation. To reveal the soil microecology response under this co-influence, three intersection areas (cornfield, vegetable field, and paddy field) were investigated. With a similar nutrient condition, the soils showed varied Cd levels (0.31-7.70 mg/kg), which was negatively related to the distance from mining water flow. Different soils showed varied microbial community structures, which were dominated by Chloroflexi (19.64-24.82%), Actinobacteria (15.49-31.96%), Acidobacteriota (9.46-20.31%), and Proteobacteria (11.88-14.57%) phyla. A strong correlation was observed between functional microbial taxon (e. g. Acidobacteriota), soil physicochemical properties, and Cd contents. The relative abundance of tolerant bacteria including Vicinamibacteraceae, Knoellia, Ardenticatenales, Lysobacter, etc. elevated with the increase of Cd, which contributed to the enrichment of heavy metal resistance genes (HRGs) and integration genes (intlI), thus enhancing the resistance to heavy metal pollution. Cd content rather than crop species was identified as the dominant factor that influenced the bacterial community. Nevertheless, the peculiar agrotype of the paddy field contributed to its higher HRGs and intlI abundance. These results provided fundamental information about the crop-specific physiochemical-bacterial interaction, which was helpful to evaluate agricultural environmental risk around the intersection of farmland and pollution sources.

Moisture content (MC) influences substance transformation during composting and the function of exogenous microbial agents. Unsuitable MC could cause leaching, nutrient loss, and secondary contamination. In this study, chicken manure composting with varied MC (45-61%) was conducted under functional microbial agent inoculation to explore the optimum condition for composting and the potential mechanism. Due to the enhanced decomposing, nitrosation, and nitrification effect lead by the functional microorganism, treatment with the optimal MC (53%) exhibited the highest composting temperature (61 degrees C) and longest high-temperature period (15 days), achieving a final carbon-nitrogen ratio (C/N), humic acids and fulvic acids ratio (HA/FA), and NH4-N+/NO3--N at 19.20, 2.00, and 0.93, respectively. After composting, the total nitrogen (TN) increased by 13.01-22.10% in the treatments with microbial agent inoculation compared with original stack, while it decreased by 7.76% in control. The highest nutrient (5.63%, 5.63-14.20% higher than the other composts) and better product safety (11.43 -23.58% higher seed germination than others) were observed in treatment with MC at 53%, exceeding the Chinese national standard for organic fertilizer. Obviously, under optimum MC, microbial agent augmentation lead to high quality and safe compost products after a short composting period (25 days) without any leaching, which suggested an efficient way to promote the recycling and recovery of husbandry waste. (C) 2020 Elsevier Ltd. All rights reserved.

Pleurotus has great potential for heavy metal mycoremediation. Using comparative transcriptome analysis, the response of Pleurotus ostreatus and Pleurotus cornucopiae under Cd contamination was evaluated. P. ostreatus and P. cornucopia accumulated 0.34 and 0.46 mg/g Cd in mycelium, respectively. Cd removal elevated with its concentration elevation, which reached 56.47% and 54.60% for P. ostreatus and P. cornucopia with Cd at 20 mg/L. Low-level Cd (≤ 1 mg/L) had no significant influence on either fungus, while varied response was observed under high-level Cd. 705 differentially expressed genes (DEGs) were identified in P. cornucopia at Cd1 and Cd20, whereas 12,551 DEGs in P. ostreatus. Differentially regulated functional categories and pathways were also identified. ATP-binding cassette transporters were involved in Cd transport in P. cornucopia, whereas the endocytosis and phagosome pathways were more enhanced in P. ostreatus. 26 enzymes including peroxisomal enzymes catalase and superoxide dismutase were upregulated in P. ostreatus, whereas only cytosolic catalase was overexpressed in P. cornucopia, suggesting their different Cd detoxification pathways. Also, the mitogen-activated protein kinase signaling pathway involved in Cd resistance in both species instead of glutathione metabolism, although more active in P. ostreatus. These findings provided new insight into the molecular mechanism of mycoremediation and accumulator screening.

Co-presence of organic pollutants and heavy metals in soil is causing increasing concerns, but the lack of knowledge of relation between soil ecology and pollutant fate is limiting the developing of specific control strategy. This study investigated soil change under pyrene stress and its interaction with cadmium (Cd). Soil physicochemical properties were not seriously influenced. However, pollutants' presence easily varied soil microbial activity, quantity, and diversity. Under high-level pyrene, Cd presence contributed to soil indigenous microorganisms' adaption and soil microbial community structure stability. Soils with both pyrene and Cd presented 7.11-12.0% higher pyrene degradation compared with single pyrene treatment. High-throughput sequencing analysis indicated the proportion of Mycobacterium sp., a commonly known PAHs degrader, increased to 25.2-48.5% in treatments from 0.52% in control. This phenomenon was consistent with the increase of PAHs probable degraders (the ratio increased to 2.86-6.57% from 0.24% in control). Higher Cd bioavailability was also observed in soils with both pollutants than that with Cd alone. And Cd existence caused the elevation of Cd resistant bacterium Limnobacter sp. (increased to 12.2% in CdCK from 2.06% in control). Functional gene prediction also indicated that abundance of genes related to nutrient metabolism decreased dramatically with pollutants, while the abundances of energy metabolism, lipid metabolism, secondary metabolites biosynthesis-related genes increased (especially for aromatic compound degradation related genes). These results indicated the mutual effect and internal-interaction existed between pollutants and soils resulted in pollutants' fate and soil microbial changes, providing further information regarding pollutants dissipation and transformation under soil microbial response.

We developed a magnetic nano-material from spent Lentinula edodes substrate (LES), which was coated with nano-magnet after carbonization to remove Cr(VI). LES was given polyporous and crinkly surface structure after carbonization and the iron modification, which significantly improved its Cr affinity. X-ray diffraction analysis verified nano-Fe3O4 crystal formation on LES biochar (LBC) surface and the possible existence of grimaldiite. X-ray photoelectron spectroscopy analysis manifested the reduction of Cr(VI) to Cr(III). The maximum removal rate of Cr(VI) by LBC-Fe3O4 reached 99.44%. Taguchi experiment indicated the removal rate was mainly determined by Cr(VI) concentration, dosage, and pH. Furthermore, LBC-Fe3O4 maintained 78.56% of its initial Cr(VI) removal efficiency after seven regenerations, indicating its fabulous stability. LBC-Fe3O4 also presented efficient removal of multi-pollutants including Cu, Ni, Pb, COD, NH4-N, etc. in practice. This research proved that LBC-Fe3O4 could be applied for the treatment of Cr(VI) influenced liquid and recycling of agricultural waste.